Genetic Diversity of ‘Ubá’ Mango Tree Using ISSR Markers

Abstract

In this study, the genetic diversity of 'Ubá' mango trees cultivated at the Zona da Mata of Minas Gerais State, Brazil, was assessed, to identify whether there is variability in the plants grown in the region, justifying the mass selection as a breeding method. We used 102 accessions. Leaves were collected for extraction of genomic DNA, which was amplified with nine ISSR primers. The data obtained by the analysis of electrophoretic patterns were arranged in a binary matrix, considering 0 for the absence and 1 for the presence of bands. Based on these data, we performed the analysis of genetic dissimilarity and carried out the cluster analysis by the methods of Tocher and graphical dispersion. The most similar accessions are 144 and 150, both coming from Ubá, while the most divergent ones are 29 and 97, from Visconde do Rio Branco. The grouping by the Tocher method separated the accessions into six groups, 94.1% of which were allocated in the first group and showed that there is no separation of accessions depending on the sampling sites. The 3D scatter plot reinforces this conclusion. There is genetic variability among the accessions of 'Ubá' mango tree evaluated. Therefore, it is possible to make mass selection in open-pollinated populations.

Morphological and molecular characterization and evaluation of mango germplasm: An overview

“…The accessions 144 and 150 were most similar, whereas, accessions 29 and 97 were most divergent in nature. All the accessions were separated into six main groups, 94.1% exhibited in the first group and it was revealed that there was no significant separation of the accessions based on sampling locations (Rocha et al, 2012).…”

Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers

“…Previous studies from India also show that mango varieties from different geographical zones though differ genetically, do not fall into distinct geographical region specific groups (Karihaloo et al 2003;Pandit et al 2007). Rocha et al (2012) also have reported that there was no accession grouping according to sample locations in their genetic diversity study of 'Uba 0 ' mango tree using ISSR markers. Observation of non differentiation of genotypes according to geographical pattern and admixtures in the populations in the current study can be attributed to more than one reason.…”

“…Molecular markers on the other hand have proved their potentiality in unraveling the limitations of morphological traits. In recent years, extensive molecular work has been carried out to estimate the genetic variation in mango germplasm using random amplified polymorphic DNA (RAPD) markers (Schnell et al 1995;Lopez et al 1997;Ravishankar et al 2000;Hemant Kumar et al 2001;Karihaloo et al 2003;Rahman et al 2007;Rajwana et al 2008;Díaz-Matallana et al 2009;Souza et al 2011;Ramessur and Ranghoo 2011) and restriction fragment length polymorphism (RFLP) analysis (Eiadthong et al 1999); amplified fragment length polymorphism (AFLP) markers (Eiadthong et al 2000;Kashkush et al 2001;Yamanaka et al 2006;Gálvez-López et al 2010) inter simple sequence repeat (ISSR) markers (He et al 2005;Pandit et al 2007;Srivastava et al 2007;Tomar et al 2011;Rocha et al 2012;Samal et al 2012) and start codon targeted (SCoT) markers (Luo et al 2010). Among the molecular markers simple sequence repeat or (microsatellites) have been found to be the marker of choice for more precisely understanding the genetic diversity, gene mapping, and cultivar discrimination due to their abundance, co-dominant and high reproducibility nature (Gupta and Varshney 2000).…”

Amadurecimento de manga 'Ubá' com etileno e carbureto de cálcio na pós-colheita

“…II especificamente na Zona da Mata Mineira, destaca-se a cultivar Ubá, que é encontrada crescendo espontaneamente em praticamente todos os municípios da região. Os frutos dessa cultivar são utilizados principalmente no processamento industrial, mas têm grande aceitação para o consumo in natura (ROCHA et al, 2011).…”