Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira, Camila Santos; Silva, A.V.C.; Alves, Rodrigo Pereira; Feitosa-Alcantara, Rosana Barroso; Arrigoni-Blank, M.F.; Álvares-Carvalho, Sheila Valéria; Costa, Tatiana Santos; White, Larissa Alves Secundo; Pinto, Vanderson dos Santos; Sampaio, Taís Santos; Blank, A.F.

doi:10.4238/gmr16029602

Cited by 17 publications

(11 citation statements)

References 30 publications

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“…These results reinforce the potential of RGA markers to access genetic polymorphism. The polymorphism results obtained with the use of ISSR primers in C. linearifolius were similar to the results presented for other species of the genus Croton, such as Croton tetradenius (94.8%) (Almeida-Pereira et al, 2017) and Croton heliotropiifolius (94%) (Rocha et al, 2016).…”

Section: Resultssupporting

confidence: 82%

Diversity and genetic structure in natural populations of Croton linearifolius (Euphorbiaceae) based on molecular markers

Silva¹,

Freitas²,

Santos³

et al. 2020

Afr. J. Biotechnol.

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Croton linearifolius Mull. Arg, an endemic species of Brazil, has insecticidal activity proven. To the detriment of its importance as a natural resource, the studies of this species are incipient, as well as the strategies applied to its management and conservation. The diversity and genetic structure in 61 individuals of C. linearifolius collected in the National Forest Contendas do Sincorá (NFCS) were estimated. Estimates were based on analysis of the amplification profile of nine combinations of pairs of resistance gene analogs (RGA) primers and eight inter simple sequence repeat (ISSR) primers. A total of 134 markers (81.3% polymorphic) were generated. Bayesian analysis indicated as most likely a structuring into two groups. Based on the molecular analysis of variance (AMOVA) it was possible to verify that 64% (p rand <0.01) of the variation occurs within the regions, and a significant amount (36%) (p rand <0.01) was attributed to variations between regions, indicating genetic structure between them. The AMOVA results were corroborated with the Principal Coordinate Analysis (PCoA), indicating an association between the distribution of variability and the geographical distribution of the collection regions. The probable mechanisms pollination and dispersion would justify, at least in part, the genetic structuring observed for C. linearifolius.

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Section: Resultssupporting

confidence: 82%

Diversity and genetic structure in natural populations of Croton linearifolius (Euphorbiaceae) based on molecular markers

Silva¹,

Freitas²,

Santos³

et al. 2020

Afr. J. Biotechnol.

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show abstract

“…The Shannon index varies from 0 to 1; the closer to zero, the lower the diversity (da . The values found in this study (I = 0.334) are lower than those obtained for C. tetradenius in the Caatinga vegetation (I = 0.450), northeast region of Brazil (Almeida-Pereira et al 2017).…”

Section: Resultscontrasting

confidence: 82%

“…Overexploitation can erode the genetic diversity of some species, promoting loss of genetic variants and endangering several of them even before they are known (Souza 2015). In this sense, germplasm characterization is important to subsidize the use of natural resources, as it allows new materials of interest to be included in genetic improvement programs and serves as a basis for the design of conservation strategies (Souza 2015;Almeida-Pereira et al 2017). Different methods are used to characterize genetic resources; among them are evaluations of morphological, chemical and molecular characteristics.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

et al. 2020

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Studies on the genetic diversity of species with pharmacological potential are essential for the conservation of genetic resources. The genetic characterization of a species can guide efforts to collect and conserve germplasm and, in the future, genetic improvement. Domestic cultivation of medicinal plants is an important strategy for protecting these species from over harvesting. Genetic studies of medicinal species by molecular markers could allow for assessment and management of these species. Croton urucurana Baill. is widely used in Brazilian traditional medicine, but there are practically no data about its genetic patterns. We used 12 ISSR markers to evaluate the genetic diversity levels of 30 trees distributed in three populations of C. urucurana, two of which were located in Brazilian savanna (Cerrado) and one on semi-deciduous tropical forest areas. From a total of 82 bands recorded, 89% were polymorphic and presented a Shannon's diversity index of 0.26. The populations formed two distinct groups: Two populations were divided each into a different genetic group, while the Aquidauana population, which is in a transition area between the Cerrado and the Pantanal, presented a mixture of the two genetic groups. Croton urucurana has G ST values (0.200) similar to those found in species with a mixed mating system, but the lack of detailed studies on the reproductive system of this species makes it difficult to correctly interpret the origin of the population structure observed. Moreover, even using a simpler marker we were able to identify regions that deserve further studies, especially from a chemical point of view, to see whether there are any differences between the populations that could help in defining future conservation strategies, management and genetic improvement plans of the species.

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“…Genetic analysis using genetic markers in plant have shown the occurrence of very high genetic diversity, especially with in populations, whereas low differentiation has been observed among populations [20]. Genetic variation and genetic relationships were efficiently determined using the ISSR markers.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker

Monfared

Samsampour

Sharifi-Sirchi

et al. 2018

Journal of Genetic Engineering and Biotechnology

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Studies on the genetic variation in marginal populations and differentiation between them are essential for assessment of best gene conservation strategies and sampling schemes. In this study, ISSR markers were used to establish the level of genetic relationships and polymorphism 50 genotypes of Salvadora persica collected from 6 different regions of Hormozgan province. The ISSR analysis with 9 anchored primers also generated 105 scorable loci, of which 85 were polymorphic (80.95%). Parameters of genetic diversity and its partitioning were calculated. The genetic analysis demonstrated that S. persica maintain relatively high genetic diversity (PIC was 0.63, Na was 1.27 and Ho and He were 0.15 and 0.17 respectively). The coefficient of genetic differentiation among populations based on FST equaled 0.20. Genetic identities between population's pairs were high (mean I = 0.88). These values are high as compared with other widespread congener species. Cluster analysis based on the Unweighted Pair Group Method with Arithmetic Averages (UPGMA) revealed 3 main clusters for the ISSR data. The levels of genetic diversity maintained within populations of S. persica indicate that an appropriate sampling design for ex situ safeguarding should capture the majority of genetic diversity found within these taxa to help ensure the long term viability of this species. Furthermore, it could be inferred that ISSR markers are suitable tools for the evaluation of genetic diversity and relationships within the Salvadora persica.

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Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Cited by 17 publications

References 30 publications

Diversity and genetic structure in natural populations of Croton linearifolius (Euphorbiaceae) based on molecular markers

Diversity and genetic structure in natural populations of Croton linearifolius (Euphorbiaceae) based on molecular markers

Genetic diversity and population structure of Croton urucurana Baill. (Euphorbiaceae) in Central Brazil by ISSR markers

Assessment of genetic diversity in Salvadora persica L. based on inter simple sequence repeat (ISSR) genetic marker

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