Determination of genetic relationships among Velezia L. Caryophyllaceae) species using RAPD markers

Poyraz, İlham Eröz; Sözen, Emel; Ataşlar, Ebru; Poyraz, İsmail

doi:10.3906/biy-1012-177

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…After PCR analyses, "1" was given if there were DNA bands on the gels and "0" was given if there were no bands; DNA bands were scored in this way, and RAPD analyses were carried out on polymorphic bands by applying 219 Table 1. Primers used in the RAPD-PCR reactions and their Tm degrees In the past studies using RAPD-PCR technique including Desmodium (Rahman et al, 2017), Calendula (Baciu et al, 2013) Vigna mungo (Arulbalachandran et al, 2010), Velezia (Poyraz et al, 2012), Hordeum vulgare (Olgun et al, 2015), Prunus (Casas et al, 1999), Malus (Botez et al, 2009), Pyrus (Monte-Corvo et al, 2000, Olea europaea ssp. oleaster (Sesli and Yegenoğlu, 2017), and Brassica napus (Özbek and Gıdık, 2013) were reported with respect to genetic diversity and phylogenetic analysis of many plant species and populations.…”

Section: Data and Phylogenetic Analysismentioning

confidence: 99%

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

2019

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In this study, we performed a genetic diversity analysis using RAPD markers for some Vitex agnus-castus populations grown in Aydin, Turkey. Total genomic DNA isolation from the leaves of Vitex agnus-castus was performed using a commercial kit. Seven RAPD primers (OPA-02, OPA-05, OPA-13, OPA-15, OPA-16, OPA-18, OPA-20) were used to determine genetic diversity among populations. Polymerase Chain Reaction (PCR) was performed with all genomic DNA samples and primers. PCR products were run in agarose gel electrophoresis and visualized under UV light. The amplified products were scored as bands (1) and no bands (0) for all gel images and their matrix files were generated. A total of 36 characters were obtained from the primers. Phylogenetic relationships and genetic distances between the cultivars were calculated by using the PAUP* (Phylogenetic Analysis Using Parsimony and other methods) program. According to PAUP analysis, the closest genetic distances were between Çine pink flower and Çakmar purple flower, and Çakmar pink flower and Çakmar purple flower populations with a value of 0.05556; and the greatest genetic distance was between Çakmar pink flower and Köşk purple flower populations with a value of 0.36111. In the phylogenetic analysis obtained using UPGMA algorithms, the phylogenetic tree consisted of four groups. The results suggest that RAPD markers are useful tools for determining genetic relationships among Vitex agnus-castus genotypes.

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Section: Data and Phylogenetic Analysismentioning

confidence: 99%

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

2019

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Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Uslu¹,

Ertuğrul²,

Babaç³

2013

Turk J Biol

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Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard's coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were 0.33 between T. hirtum All. and T. constantinapolitanum Ser. for RAPD and 0.49 between T. medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.

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The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach

Erol¹,

Kaya²,

Şık³

et al. 2014

Turk J Biol

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In this study, a total of 26 Crocus specimens from different locations across Turkey and 2 East Aegean islands (Chios and Samos) were analyzed using 12 amplified fragment length polymorphism (AFLP) primer combinations to obtain information on genetic diversity, population structure, and genetic relationships. A total of 369 polymorphic AFLP bands were generated and scored as binary data. Genetic similarities were determined. Cluster analysis revealed 4 major groups among the 26 genotypes examined in this study. The nuclear DNA contents (2C) of the 26 Crocus specimens were found to range from 5.08 pg in C. asumaniae to 9.75 pg in C. sativus. Polymorphic information content (PIC) values were used to examine the capacity of the various primer pairs to amplify polymorphisms in the Crocus specimens. The PIC values ranged from 0.218 (M-CAA/E-AGC) to 0.512 (M-CAT/E-AAG) and showed an average of 0.34. In sum, we herein used AFLP analysis to identify a high level of polymorphism among Crocus specimens collected from various locations in Turkey and Greece, and our structural analysis yielded 2 reconstructed populations. These findings provide new insight into the relationships among different Crocus genotypes and show that AFLP analysis can be useful for Crocus diversity studies.

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Determination of genetic relationships among Velezia L. Caryophyllaceae) species using RAPD markers

Cited by 6 publications

References 18 publications

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

Molecular Characterization of Vitex agnus-castus L. (Verbenaceae) Populations Grown in Aydin, Turkey

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

The genus Crocus, series Crocus (Iridaceae) in Turkey and 2 East Aegean islands: a genetic approach

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