Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata)

Abstract

Research has been done to investigate the levels of flavanoid, tannin, and vitamin C of methanol extract of Stone banana (Musa brachycarpa), Ketip banana (Musa paradisiaca forma typiaca ), and Kepok banana (Musa acuminata). Jukut Ares is a Balinese traditional cuisine frequently served in any events of celebrations. The use of young stems of such banana varieties as the main raw materials in the process of making jukut ares has not been scientifically elucidated. Antioxidants are compounds with ability to inhibit oxidation-related reactions due to free radicals that cause disturbance of saturated fatty acids of cell membrane, blood vessels, DNA, as well as adipose tissues which lead to occurrence of various diseases. Plants components will have antioxidant activities if they contain compounds (phenol and flavanoid) with ability to scavenge free radicals. Tannin is a water based phenolic compound and vitamin C is also a water based vitamin that play important roles in preventing us from various diseases. Extraction was conducted by applying maceration method and to investigate the levels of flavanoid, spectrophotometric UV vis with aluminum chloride (AlCl3) was applied. The level of vitamin C of the materials was conducted by Iodine titration method, while the level of tannin was determind by applying spectrophotometri. The results showed that methanol extract of Ketip banana stem contained the highest level of flavanoid with a quantity of 53.13 mg/100gr QE, and this was followed by stone banana (36.28) and kepok banana (32.07) The highest vitamin C level was indicated in the extract of ketip banana (418.32 mg/100gr), which is followed by kapok banana and stone banana. In the calculation of tannin content, pisang ketip showed the highest level, while pisang batu contained the lowest level of tannin.

Comparative study of catechin levels from green tea, oolong tea and black tea product with various treatments

“…The results in Table 1 show that the sample gave a positive reaction to form a black-green and red-orange complex with the addition of 1 (%w/v) FeCl3 reagent. This reaction indicates the presence of gallocatechin [13,14].These results are also reinforced by by using a steasny reagent (formaldehyde 20%: HCl p (2: 1)) which showed a red-black colour change. The colour change of the sample shows that it contains catechin tannins [14,15].…”

“…Catechin identification was carried out using FeCl3 and steasny reagents (Formaldehyde 20 (%v/v) and Concentrated HCl 2: 1) as well as the UV spectrophotometer profile of each sample. Catechins are polyphenolic compounds that can form complexes between phenol (-OH) groups from the sample in the presence of Fe 3+ (iron), characterized by the formation of a complex color change to green, blue, or red to black [13]. Table 1 shows the color changes that occurred in each sample using different extraction methods.…”

The extract of Cavendish banana (Musa acuminata) peel as antioxidant and anti-aging agents in model yeast Schizosaccharomyces pombe

“…Kulit pisang Cavendish tipis dan berwarna kuning muda saat matang. Kulit pisang dilaporkan memiliki kandungan senyawa flavonoid yang cukup tinggi (Putu et al, 2017). Bahkan, konsentrasi flavonoid kulit pisang ambon sebesar 818 mg 100 g -1 lebih tinggi dari blueberry yaitu sebesar 30,44 mg 100 g -1 yang dikenal memiliki senyawa antioksidan yang tinggi (Parvez et al, 2023).…”

“…Hasil analisis fitokimia menunjukkan bahwa ekstrak kulit pisang uli tersebut memiliki kandungan flavonoid dan tannin. Flavonoid juga dilaporkan terkandung dalam ekstrak pisang kapok (Musa acuminata) dan pisang ketip (Musa paradisiaca forma Typiaca) (Putu et al, 2017). Aktivitas antioksidan dari kelompok senyawa flavonoid telah banyak dilaporkan diantaranya berasal dari ekstrak tumbuhan Guazuma ulmifolia (Syaefudin et al, 2014), Euphorbia splendida (Kefayati et al, 2017), Clinacanthus nutans (Ismail et al, 2017), lotus (Tungmunnithum et al, 2022), dan Adenostemma lavenia (Batubara et al, 2020).…”