Comparative study of catechin levels from green tea, oolong tea and black tea product with various treatments

Abstract

Background: Tea is a refreshing drink that contains polyphenol compounds, namely catechins that are used for medicine and cosmetics. This study was to assess the content of catechin compounds in green tea, oolong and black tea products from Indonesia, China and Taiwan. Methods: Some tea products are brewed at varying temperatures (75±2; 85±2 and 95±20 C) and times (5; 10 and 15 minutes). Identification of catechin compounds was carried out using chemical reagents and UV spectrophotometry. The level of cathecin in tea products were analyzed by spectrophotometer at 280 nm wavelength. Results: The results obtained indicate that green tea, oolong tea and black tea contain epigallocatechin-3-gallate (EGCG) compounds according to the color change based on chemical reagents and for UV spectrum analysis which has λmax in the range 268-274 nm. The results of quantitative tests using UV-Vis spectrophotometry showed that the green tea samples gave the highest levels of catechins followed by oolong tea and black tea with brewing temperature at 95±20 °C. Conclusion: The catechin content of tea obtained from various products varies according to the type of processing method and the brewing temperature. Therefore, this study is expected to provide information related to catechin content to the public and researchers.

UV-Vis Spektrofotometrisi ile Geleneksel Bitkisel Ürünlerde (Jamu) Tıbbi Kimyasalların Doğrulanması

“…The comparison of standards M and PC can be identified by looking at the maximum wavelength in the UV region. M and PC are chemical compounds containing aromatic molecules that are good chromophores to be identified using a UV spectrophotometer [12]. The search results can be seen in Figure 2 and Figure 3.…”

“…The analysis carried out to determine the presence of medicinal chemicals in traditional medicine (Jamu) can be done using the thin layer chromatography method to see the stain profile at UV 254 and UV 366 and UV-Vis spectrophotometry [1,11,12]. These two methods often analyze medicinal chemicals in traditional medicinal products qualitatively and quantitatively based on the absorbance read in spectrophotometry.…”