Derivation of Cinnamon Blocks Leukocyte Attachment by Interacting with Sialosides

Abstract

Molecules derived from cinnamon have demonstrated diverse pharmacological activities against infectious pathogens, diabetes and inflammatory diseases. This study aims to evaluate the effect of the cinnamon-derived molecule IND02 on the adhesion of leukocytes to host cells. The anti-inflammatory ability of IND02, a pentameric procyanidin type A polyphenol polymer isolated from cinnamon alcohol extract, was examined. Pretreatment with IND02 significantly reduced the attachment of THP-1 cells or neutrophils to TNF-α-activated HUVECs or E-selectin/ICAM-1, respectively. IND02 also reduced the binding of E-, L- and P-selectins with sialosides. Furthermore, IND02 could agglutinate human red blood cells (RBC), and the agglutination could be disrupted by sialylated glycoprotein. Our findings demonstrate that IND02, a cinnamon-derived compound, can interact with sialosides and block the binding of selectins and leukocytes with sialic acids.

The Structure of Leukocyte Sialic Acid-Containing Membrane Glycoconjugates is a Differential Indicator of the Development of Diabetic Complications

“…Selectins are carbohydrate-binding proteins that recognize the sLe X structure (Neu5Acα2,3Galβ1,4(Fucα1,3)GlcNAcβ-R), capping N-and O-glycans as specific ligands [51][52][53]. These sialic acid-containing moieties are required for leukocyte binding to selectins on endothelial cells and their rolling [54,55]. Combinatorial knockout of ST3Gal-IV and ST3Gal-VI that are the involved in sLe X synthesis leds to a decrease in neutrophil binding to E-and P-selectins, selectin-dependent rolling, and lymphocyte homing [46].…”

Cinnamon and its possible impact on COVID-19: The viewpoint of traditional and conventional medicine

“… Interacting with sialosides and blocking the binding of selectins and leukocytes with sialic acids were observed. [58] 10 Type-A procyanidin polyphenols Bark In vitro isolated rat peritoneal mast cells - The number of degranulated cells and levels of markers (histamine, β-HEX, and IL-4) was decreased in a dose-dependent manner. Mast cell was stabilized, and the allergic markers such as histamine, IL-4, and β-HEX in an IgE-mediated manner were inhibited.…”

Effect of Agmatine on Actin Polymerization Dynamics in Wga-Stimulated Leukocytes Under Diabetes Mellitus in Rats

“…actin-binding proteins), що сприяє полі меризації актину [1,14,17]. Отже, перерозподіл на поверхні лейкоцитів сіало-глікокон'югатів і встановлення взаємо-зв'язку із внутрішньоклітинними сигналь-ними молекулами і, особливо, ABPs, впли-ває на функціональну активність лейкоцитів [18,19].…”