Demineralized Freeze-Dried Bovine Cortical Bone: Its Potential for Guided Bone Regeneration Membrane

Abstract

Background Bovine pericardium collagen membrane (BPCM) had been widely used in guided bone regeneration (GBR) whose manufacturing process usually required chemical cross-linking to prolong its biodegradation. However, cross-linking of collagen fibrils was associated with poorer tissue integration and delayed vascular invasion. Objective This study evaluated the potential of bovine cortical bone collagen membrane for GBR by evaluating its antigenicity potential, cytotoxicity, immune and tissue response, and biodegradation behaviors. Material and Methods Antigenicity potential of demineralized freeze-dried bovine cortical bone membrane (DFDBCBM) was done with histology-based anticellularity evaluation, while cytotoxicity was analyzed using MTT Assay. Evaluation of immune response, tissue response, and biodegradation was done by randomly implanting DFDBCBM and BPCM in rat's subcutaneous dorsum. Samples were collected at 2, 5, and 7 days and 7, 14, 21, and 28 days for biocompatibility and tissue response-biodegradation study, respectively. Result DFDBCBM, histologically, showed no retained cells; however, it showed some level of in vitro cytotoxicity. In vivo study exhibited increased immune response to DFDBCBM in early healing phase; however, normal tissue response and degradation rate were observed up to 4 weeks after DFDBCBM implantation. Conclusion Demineralized freeze-dried bovine cortical bone membrane showed potential for clinical application; however, it needs to be optimized in its biocompatibility to fulfill all requirements for GBR membrane.

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction

“…This downward pattern of PMN cells confirmed that there was no prolonged inflammatory response. 19 This clearance process was also marked by the release of the tissuerepairing cytokines, transforming growth factor-β (TGFβ) and interleukin-10 (IL-10), which initiates tissue repair. Thus, substances that promote PMN cells' apoptosis have a therapeutic potential to accelerate wound healing and tissue repair.…”

Effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressed by osteoblasts in the tooth extraction sockets of Cavia cobaya

“…The removal of minerals contained in the bone was effected by soaking it in an acidic solution. 5 More recently, however, treatments have been developed and improved by the use of drugs derived from plants or herbs whose medicinal properties have been known for many years. One such medicinal herb is Moringa oliefera which can be used to accelerate the process of bone formation and prevent that of bone resorption.…”

In Vivo Analyses of Osteogenic Activity and Bone Regeneration Capacity of Demineralized Freeze-Dried Bovine Bone Xenograft: A Potential Candidate for Alveolar Bone Fillers

“…e major advantage of DFDBBX, compared to DFDBA, is its high availability, while its main drawback is its possibility of xenogenic reaction. However, few animal studies demonstrated no immune reactions of bovine bone-derived 4 International Journal of Dentistry material during implantation [9,15]. Taking into consideration the advantages and disadvantages of DBBM and DFDBBX particles, this study compared their osteogenic capacities.…”

“…e DFDBBX, similar to DFDBA, is bovine bone processed with acidic decalcification eliminating the mineral content and retaining the organic component containing some osteoinductive growth factors [8]. Many had concern over the potential xenogeneic response to the bovine bone; however, an in vivo study found that the organic part of the bovine bone was neither antigenic nor immunogenic [9]. Nevertheless, the bone regenerative capacity of DFDBBX granule as bone filler has to be proven.…”