Effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressed by osteoblasts in the tooth extraction sockets of Cavia cobaya

Abstract

Background: Tooth extraction is a common procedure in dentistry after which the residual ridge will no longer receive stimulus leading to volume, height and width loss. These anatomical changes can then result in difficulties with future denture fabrication and implant placement. Preservation of the alveolar ridge, therefore, assumes considerable importance after tooth extraction. Moringa oleifera, on the other hand, can enhance bone formation. Type-1 collagen is a marker of osteoblast formation. Purpose: This research aimed to analyze the effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressions in tooth extraction sockets. Methods: 56 Cavia cobaya subjects were divided into eight groups. Their lower left incisors were then extracted prior to the sockets of the first and fifth groups being filled with PEG, those of the second and sixth groups with DFBBX, those of the third and seventh groups with Moringa oleifera leaf extract and a combination of DFBBX and Moringa oleifera leaf extract in those of the fourth and eighth groups. The sockets were then examined on days 7 and 30 by means of an immunohistochemical technique. The data collected was subsequently subjected to analysis by One Way Anova and Tukey HSD tests. Results: There were significant differences between the control group and the treatment group administrated with Moringa oleifera leaf extract combined with DFBBX. On days 7 and 30, the groups treated with the combination of DFBBX and Moringa oleifera leaf extract had the highest number of type-1 collagen expressions. Conclusion: A combination of DFBBX and Moringa oleifera leaf extract is effective in increasing type-1 collagen expressions in tooth extraction sockets.

Improvements of alveolar bone healing using Moringa oleifera leaf powder and extract biomimetic composite: an experimental study in dogs

“…This is on account of phytochemical contents. 29 This was concurrent with an invivo study conducted on the effect of Moringa oleifera Lam. leaf ethanolic extract on osteogenic differentiation of porcine bone marrow derived mesenchymal stem cells showed positive results.…”

“…16 Previous studies confirmed the tissue preservation of M. oleifera leaf ethanolic extracts, for example it protected rats against the hepatotoxicity of various drugs, 9 and increased cell proliferation and cell migration of human dermal fibroblasts. 29 A study 2010 was the first described using MO leaf ethanol extract in feeding ovariectomized rat and concluded osteoprotection, 23 this was confirmed by a study conducted on osteoporotic women which concluded increased bone density after consumption of MO leaf. 30 Further researches demonstrated MO leaf local effects on bone healing and osteoblast cells, started by an invivo study using different part of MO extracts (leaf-fruit-flower) on osteoblast cells, this results demonstrated negative effect of leaf extract on osteoblast and supports the fruit and flower positive effect.…”

“…Going into some details, the MO leaf extract increased alkaline phosphatase activity, collagen type I expression, osteocalcin and transforming growth factor beta (TGF B ) expressions (responsible for deposition of bone matrix ), besides it decreased number of Rankle expression . 1,24,29,31,32 Moreover, a study conducted on upper central extraction socket in a rat model 2018 using mate tea intra-gastric lavage concluded positive effects on alveolar healing at 28 days postoperatively. 4 Our study uniquely used high concentration of water extract (11.7%) suggested to be comparable to ethanol osteoprotective property.…”

“…Polyphenols decrease inflammatory mediators, such as histamine, and cytokines. Consequently decrease bone resorption 29,[34][35][36] Chlorogenic acid, rutin and ferulic acid had osteoprotective function by promoting bone mineral density. [37][38][39][40][41] Gallic acid in an in-vivo study showed positive effects around implants .…”

Induction effect combination of Nigella sativa extract and bovine bone graft to the area of woven bone on the preservation socket post-tooth extraction

“…The preservation socket aims to compensate biological resorption of the buccal bone wall, maintain bone volume and bone structure so that it can function optimally and obtain good esthetics. [1,2]. Bone graft is a material that can stimulate bone healing because it has osteogenic, osteoinductive and osteoconductive properties (Nguyen Ngoc Hung, 2012).…”