Cytotoxicity evaluation of three resin-based sealers on an L929 cell line

Abstract

Background:Endodontic sealers usually come in contact with adjacent tissues and their biocompatibility is key in a successful treatment. The purpose of this study was to assess the cytotoxicity of three resin-based sealers, namely AH Plus, EndoREZ, and Epiphany in set and fresh states on an L929 cell line.Materials and Methods:In this in vitro experimental study, the materials were mixed according to the manufacturers’ instructions, and were divided into two groups, fresh and set. The elutes of materials were prepared separately and were incubated with L929 fibroblasts for 1 hour, 24 hours, and 72 hours. Pulp Canal Sealer and Dulbecco's Modified Eagle Medium (DMEM) served as positive and negative controls respectively. Cell viability was evaluated by MTT assay ([3-4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide succinate), after 1 hour, 24 hours, and 72 hours. The data were analyzed by analysis of variance (ANOVA), and Tukey multiple comparison test.Results:After 1 hour, fresh Epiphany and fresh AH Plus were significantly more cytotoxic than their set samples. No significant difference was perceived between cytotoxicity of fresh state of sealers and positive control, or between set state and negative control. After 24 hours, both fresh and set samples of all materials were significantly more cytotoxic than the negative control group, and were less cytotoxic than the positive control group. After 72 hours, the fresh and set samples of all materials were as cytotoxic as the positive control group. At each time point, no significant difference was perceived among different materials in terms of cell viability.Conclusion:The observed differences among the cytotoxicity of AH Plus, EndoREZ, and Epiphany did not reach a significant level at comparable time points after exposure.

Positively charged imidazolium‐based ionic liquid‐protected silver nanoparticles: a promising disinfectant in root canal treatment

“…, Ashraf et al . ). As demonstrated in Table , the medicaments inhibited the viability of fibroblasts in a time‐dependent manner.…”

Comparison of cytotoxicity, genotoxicity and immunological inflammatory biomarker activity of several endodontic sealers against immortalized human pulp cells

“…After isolation and validation of immortalized pulp cells, the biocompatibility of contemporary sealers was tested for cytotoxicity, genotoxicity and evaluated under inflammatory aspectscytokine stimulation. Cytotoxicity was evaluated by MTT assay (3-[4,5dimethyltiazol-2-yl]-2,5-diphenyltetrazolium bromide), which has been used to test the biocompatibility of endodontic sealers (Huang et al 2002a,b, Brackett et al 2008, Ashraf et al 2012, Bin et al 2012, Scelza et al 2012, Silva et al 2013, Accardo et al 2014, Camargo et al 2014, Cotti et al 2014. Reported findings indicated that Real Seal had the highest cytotoxicity against immortalized pulp cells, which indicated a reduction by 53.8% in cell viability.…”

Antimicrobial and cytotoxic activity ofFerula gummosaplant essential oil compared to NaOCl and CHX: a preliminaryin vitrostudy

“…The reason to choose this cell line is because it is a well-characterized cell model and has been previously used to assess the cytotoxic effects of dental materials. 33 , 34 To the best of our knowledge, limited data is available in literature regarding the cytotoxicity of FGEO against specific cell lines. In an only found report by Gharaei et al, the FG leaf and flower extracts (at concentrations of 50 and 60 µL/mL, respectively) could resulted in 50% cell death on the human gastric cancer cell line (AGS).…”