Antimicrobial and cytotoxic activity ofFerula gummosaplant essential oil compared to NaOCl and CHX: a preliminaryin vitrostudy

Abstract

ObjectivesThe usage of medicinal plants as natural antimicrobial agents has grown in many fields including dental medicine. The aim of this in vitro study was three-fold: (i) to determine the chemical compositions of the Ferula gummosa essential oil (FGEO), (ii) to compare the antimicrobial efficacy of the oil with sodium hypochlorite (NaOCl) and chlorhexidine (CHX), (iii) to assess the toxic behavior of FGEO in different concentrations compared to 5% NaOCl and 0.2% CHX.Materials and MethodsGas chromatography/mass spectrometry (GC/MS) was used to determine the chemical compositions of the oil. The disk diffusion method and a broth micro-dilution susceptibility assay were exploited to assess the antimicrobial efficacy against Enterococcus faecalis, Staphylococcus aureus, Streptococcus mitis, and Candida albicans. The cytocompatibility of the FGEO was assessed on L929 fibroblasts, and compared to that of NaOCl and CHX.ResultsTwenty-seven constituents were recognized in FGEO. The major component of the oil was β-pinene (51.83%). All three irrigants significantly inhibited the growth of all examined microorganisms compared to the negative control group. FGEO at 50 µg/mL was effective in lower concentration against Enterococcus faecalis than 5% NaOCl and 0.2% CHX, and was also more potent than 0.2% CHX against Candida albicans and Staphylococcus aureus. FGEO was a cytocompatible solution, and had significantly lower toxicity compared to 5% NaOCl and 0.2% CHX.ConclusionsFGEO showed a promising biological potency as a root canal disinfectant. More investigations are required on the effectiveness of this oil on intracanal bacterial biofilms.

Natural Therapeutic Options in Endodontics - A Review

“…They proved that Ferula gummosa essential oil was effective against E. faecalis than NaOCl and CHX and it was better than CHX against C. albicans and S. aureus . The antimicrobial activity might be attributed due to the presence of β-pinene and α-pinene [ 41 ]…”

Tributyl tetradecyl phosphonium chloride for biofouling control in reverse osmosis processes

“…The results are summarized in Table 1 [44,45]. In general, TTPC exhibited lower MIC and MBC values than DBNPA for both bacterial species, implying that lower concentrations of TTPC are needed to inhibit or kill the bacteria than DBNPA.…”

Antiseptic solutions modulate the paracrine‐like activity of bone chips: differential impact of chlorhexidine and sodium hypochlorite

“…, Abbaszadegan et al. ). Furthermore, high concentrations of hydrogen peroxide may decrease survival rate of human gingiva fibroblast (Furukawa et al.…”

“…Antiseptic solution not only reduces bacterial load but they also have cytotoxic effects on eukaryotic cells. For example povidone-iodine is toxic to epithelial cells (Sato et al 2014), and chlorhexidine and sodium hypochlorite are toxic to mesenchymal cells (Proksch et al 2014, Abbaszadegan et al 2015. Furthermore, high concentrations of hydrogen peroxide may decrease survival rate of human gingiva fibroblast (Furukawa et al 2015).…”