Comparison of cytotoxicity, genotoxicity and immunological inflammatory biomarker activity of several endodontic sealers against immortalized human pulp cells

Martinho, Frederico Canato; Camargo, Samira Esteves Afonso; Fernandes, Antônio Carlos; Campos, Melina; Prado, Renata Falchete do; Camargo, Carlos Henrique; Valera, Márcia Carneiro

doi:10.1111/iej.12785

Cited by 21 publications

(25 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Apexit Plus shows low or no cytotoxicity effect on L929 mouse fibroblasts [10] and human periodontal ligament fibroblasts [9]. Moreover, no genotoxicity was induced by Apexit Plus on an immortalized cell line derived from human pulp [38]. Our morphological analysis showed that inflammatory cells were gradually replaced by fibroblasts distributed among bundles of collagen fibers in the capsules of all groups over time.…”

Section: Biological Propertiesmentioning

confidence: 72%

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

et al. 2020

View full text Add to dashboard Cite

We investigated the physical properties, antimicrobial activity, and tissue reaction to Apexit Plus in comparison to Sealapex. Flow, radiopacity, setting time, and solubility were evaluated in each material. The antimicrobial activity against Enterococcus faecalis was performed. Polyethylene tubes containing Apexit Plus or Sealapex, and without material (control group) were implanted into the subcutaneous tissue of rats. At 7, 15, 30, and 60 days of implantation, the specimens were paraffin-embedded and the number of inflammatory cells (ICs) and the amount of birefringent collagen (BC) were quantified. The von Kossa reaction followed by immunohistochemistry for detection of alkaline phosphatase (ALP) was also performed. Statistical analysis was performed with ANOVA and Tukey test (p ≤ 0.05). The flow value of Apexit Plus was greater than Sealapex, whereas the radiopacity (3.44 mm Al) was lower than Sealapex (6.82 mm Al). Apexit Plus showed lower solubility and shorter initial and final setting (p < 0.0001), whereas the antimicrobial activity was significantly greater than Sealapex. Although the number of ICs was higher in Apexit Plus (p = 0.0009) at 7 days, no significant difference was detected between Apexit Plus and Sealapex at 15, 30, and 60 days. All groups showed higher values for BC in the capsules over time. ALP-immunolabelled cells were observed, mainly around von Kossa-positive structures, either in the capsules of Apexit Plus or Sealapex. Therefore, our results revealed that Apexit Plus exhibited a greater effectiveness against Enterococcus faecalis and better physical properties than Sealapex, except for the radiopacity. In vivo findings indicate that Apexit Plus is biocompatible and presents potential bioactivity in the subcutaneous tissue.

show abstract

Section: Biological Propertiesmentioning

confidence: 72%

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

et al. 2020

View full text Add to dashboard Cite

show abstract

“…On the other hand, Martinho et al . (), using pulp cells in contact with extracts of different sealers, among them AHP, found higher levels of IL‐6 and an absence of difference for IL‐12 when compared to the control group. Those differences could be related to the cell model used, experimental design regarding sealer contact with cells and sealer composition.…”

Section: Discussionmentioning

confidence: 95%

“…Controversy exists regarding the choice of permanent cell lines or primary cell cultures as study models (Honegger , Martinho et al . ). Despite the greater use of permanent lineage cultures, these have unnatural behaviour, altering the biological responses against materials (Honegger ).…”

Section: Discussionmentioning

confidence: 97%

Evaluation of apoptosis/necrosis and cytokine release provoked by three root canal sealers in human polymorphonuclears and monocytes

Souza¹,

Rosatto²,

Silva³

et al. 2018

Int Endodontic J

View full text Add to dashboard Cite

Aim To evaluate the in vitro cytotoxicity and cytokine release of three fresh root canal sealers and to determine the type of cell death they induce. Methodology The sealers tested were Sealer 26 (S26), AH Plus (AHP), and Endosequence BC Sealer (END). Fresh sealers were cultivated in contact with monocytes and polymorphonuclears (PMNs) obtained from the peripheral blood of humans. Cell viability, apoptosis and necrosis were analysed at 4 h (PMNs) or 24 h (monocytes) using Annexin‐V and propidium iodide in a cytometer. The supernatants were used to quantify Interleukin (IL)‐4, IL‐6, IL‐10, IL‐12 and tumour necrosis factor‐α (TNF‐α) in monocytes and IL‐8 in PMNs by ELISA. One‐way ANOVA and the Tukey post‐test were used to compare data for cytotoxicity, and the multiple T‐test was used to determine the differences between sealers in the release of cytokines that were statistically significant. Results After 4 h of treatment, S26 was associated with greater cell viability than the other sealers (P < 0.05) in the PMN culture and had similar values of necrosis as END (P > 0.05). After 24 h of treatment, AHP and END had greater monocyte cell viability than S26 (P < 0.05), which had more necrosis (P < 0.05). END had the lowest levels of IL‐12 compared to the other sealers (P < 0.05) and higher levels of IL‐6 compared to S26 (P < 0.05). The tested sealers did not differ in the release of IL‐8, IL‐10, TNF‐α and IL‐4 (P > 0.05). Conclusions The effect of toxic agents released varied depending on the cell type studied. The composition of the sealers appeared to alter the form of self‐regulation in the production of these cytokines by cells.

show abstract

“…After setting, the toxicity decreases and may be null after 2 weeks. 12,13,20 In this study, the samples to obtain the extracts were used after setting, even so, the AH Plus commercial sealer (0%) showed a reduction in HGF cell viability due to a higher concentration of substances released for the medium in 7 days. In addition, the incorporation of the AgVO 3 into AH Plus in higher concentrations (5 and 10%) decreased the cell viability.…”

Section: Discussionmentioning

confidence: 99%

“…The components of the sealers may present cytotoxicity to human and animal cells, such as zinc oxide, eugenol, formaldehyde, resinous components, and calcium hydroxide, 12 causing inflammation and affecting DNA, thereby leading to genome instability and promoting carcinogenesis. 13 Within the nanoparticles, these can interact with cellular components, including DNA, proteins, and lipids, which may damage the integrity of the membrane depending on the chemistry of its surface. 14 The cytotoxicity of AgVO 3 to Daphnia similis, an aquatic organism, was evaluated by Artal et al 15 who attributed the cytotoxic effect observed to silver.…”

mentioning

confidence: 99%

Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate

et al. 2021

View full text Add to dashboard Cite

The cytotoxic and genotoxic effects of commercial endodontic sealers (AH Plus, Sealer 26 and Endomethasone N) incorporated with nanostructured silver vanadate decorated with silver nanoparticles (AgVO 3at concentrations 2.5, 5, and 10%) on human gingival fibroblast (HGF), and the silver (Ag + ) and vanadium (V 4+ /V 5+ ) ions release were evaluated. Cytotoxicity, cell death, and genotoxicity tests were carried out with extract samples of 24-hr and 7-days. The release of Ag + and V 4+ /V 5+ was evaluated. Cytotoxicity in HGF was caused by AH Plus (AP) with 5 and 10% of AgVO 3 (83.84 and 67.49% cell viability, respectively) with 24-hr extract (p < 0.05), as well as all concentrations of AP with 7-days extract (p < 0.05 -AP 0% = 73.17%; AP 2.5% = 75.07%; AP 5% = 70.62%; AP 10% = 68.46% cell viability). The commercial sealers Sealer 26 (S26) and Endomethasone N (EN) were cytotoxic (p < 0.05 -S26 0% = 34.81%; EN 0% = 20.99% cell viability with 7-days extract). AP 10% with 7-days extract induced 32% apoptotic cells in HGF (p < 0.05). Genotoxic effect was not observed. The AP groups released more Ag + , while S26 and EN released more V 4+ /V 5+ in 24 hr. The Ag + can be cytotoxic. In conclusion, the cytotoxicity caused to HGF can be attributed by the commercial sealers and enhanced by incorporation of AgVO 3 , was not observed genotoxic effect, and apoptosis was induced only by AH Plus 10% 7-days extract. Ag + can influence cell viability.

show abstract

Comparison of cytotoxicity, genotoxicity and immunological inflammatory biomarker activity of several endodontic sealers against immortalized human pulp cells

Cited by 21 publications

References 48 publications

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Physical Properties, Antimicrobial Activity and In Vivo Tissue Response to Apexit Plus

Evaluation of apoptosis/necrosis and cytokine release provoked by three root canal sealers in human polymorphonuclears and monocytes

Cytotoxic and genotoxic effects in human gingival fibroblast and ions release of endodontic sealers incorporated with nanostructured silver vanadate

Contact Info

Product

Resources

About