Cytotoxic Effect of Polyisoprenoids from Rhizophora mucronata and Ceriops tagal Leaves against WiDr Colon Cancer Cell Lines

Sari, Dini Permata; Basyuni, Mohammad; Hasibuan, Poppy Anjelisa Zaitun; Wati, Ridha; Sumardi, -

doi:10.17576/jsm-2018-4709-02

Cited by 11 publications

(3 citation statements)

References 35 publications

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“…Mangroves plants are known to produce a varied range of secondary metabolites such as sterols, ubiquinones, isoprenoids, and polyisoprenoids. Polyisoprenoid comprised polyprenol and dolichol, which is efficacious to have anticancer and antibacterial activities [1], [2], [3]. The composition and diversity of polyisoprenoids compounds in North Sumatran mangrove forests, Indonesia, have been described [4], [5].…”

Section: Introductionmentioning

confidence: 99%

Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

Istiqomah¹,

Basyuni²,

Hasibuan³

2020

Open Access Maced J Med Sci

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BACKGROUND: Mangroves secondary metabolites are mostly consisting of sterols, ubiquinones, isoprenoids, and polyisoprenoids. Polyisoprenoid is divided into two types, namely, polyprenol and dolichol, which has been reported to have biological and pharmacological activities. AIM: This research was aimed to analyze apoptosis 48 h with double staining and immunocytochemistry (ICC) 48 h of P53 and cyclooxygenase-2 (COX-2) gene expression from chemical constituents of dolichol in three mangrove leaves of Ceriops tagal, Nypa fruticans, and Rhizophora mucronata. METHODS: Apoptosis with the double-staining method was employed to analyze the genes expression in growth and development of cancer cells, P53, and COX-2 with ICC and flow cytometry method. The data were statistically analyzed using one-way ANOVA parametric statistical analysis followed by Duncan’s test. RESULTS: The result revealed that the increased apoptosis of samples C. tagal was 70% fluorescence orange, while N. fruticans and R. mucronata were 35% and 30% fluorescence orange, respectively. However, it was compared with the positive control; it produced orange fluorescent as much as 75%, suggesting that C. tagal have a position similar to 5-FU. Predominance dolichol in N. fruticans and C. tagal leaves led the expression gene of p53 to have 1.57% M1 phase, indicating the domination in G0-G1 phase (70–80%). Inhibit the expression for 48 h in p53 and COX-2 showing that n-hexane extract of C. tagal had the most percentage (80.733 ± 0.11%) to upregulate the p53 and less percentage (20.16 ± 1.19%) to downregulate the COX-2, indicating positive extract belong to N. fruticans and R. mucronata leaves. CONCLUSION: The present study confirmed the pharmacological properties of dolichol from three mangrove leaves as an anticancer of tumor suppressor genes and significantly proliferated of cancer cell growth from mangrove leaves.

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Section: Introductionmentioning

confidence: 99%

Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

Istiqomah¹,

Basyuni²,

Hasibuan³

2020

Open Access Maced J Med Sci

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“…MTT assay ; MTS assay [48][49][50]; WST assay [26]; SRB assay [51]; apoptosis with double staining method [34,45,47,52]; cell cycle analysis [53] gene expression analysis [36,37]; mitochondrial membrane potential Ψm (MMP), cytochrome c release analysis and NFkB translocation [49]; caspase activity and inhibitor assays [49]; protein extraction, protein array and western blotting analyses [49]; computational molecular docking and statistical analyses [45,50] HT-29 [24,49,50,54]; HCT-15 [26,27,43]; Colo205 [48]; WiDr [28,[30][31][32]34,[36][37][38][39][40][41][42]44,45,53]; HCT-116 [29,33,35,46,47,51]; CaCo-2 [49] Catechin 7-O-apiofuranoside and didesmethyl tocotrienol…”

Section: In Vitromentioning

confidence: 99%

Current Status, Distribution, and Future Directions of Natural Products against Colorectal Cancer in Indonesia: A Systematic Review

et al. 2021

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In 2020, an estimated 19.3 million new cancer cases and nearly 10 million cancer deaths have occurred worldwide, with colorectal cancer ranking as the third most frequently diagnosed (10.0%). Several attempts have been conducted against cancer, including surgery, radiation, monoclonal antibodies, and chemotherapy. Many people choose natural products as alternatives against cancer. These products will not only help in human life preservation but also work as a source of up-to-date information, leading people away from incorrect information. We discuss the current status, distribution, and future implications of protecting populations with natural products as an alternative against colorectal cancer in Indonesia. Thirty-eight studies were included in this review for data extraction. The distribution of natural products in Indonesia that have potential activity against colorectal cancer cells was predominated by terpenoids, followed by phytosterols, phenolics, alkaloids, and polyisoprenoids. The type of cell line utilized in the cytotoxic activity analysis of natural products was the WiDr cell line, followed by HT-29 cells and HCT-116 cells. This review showed that MTT in vitro assay is a general method used to analyze the cytotoxic activity of a natural product against colorectal cancer cells, followed by other in vitro and in vivo methods. The systematic review provided predictions for several secondary metabolites to be utilized as an alternative treatment against colorectal cancer in Indonesia. It also might be a candidate for a future co-chemotherapy agent in safety, quality, and standardization. In addition, computational methods are being developed to predict the drug-likeness of compounds, thus, drug discovery is already on the road towards electronic research and development.

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“…The reaction was stopped with 10% SDS reagent (Sigma Aldrich) in 0.01 N HCl (Merck). The plate was wrapped to protect it from the light so that the wells were opaque, and it was left overnight at room temperature 14 . Absorption was measured by the ELISA reader (Benchmark 10431, BioRad) at a wavelength of 595 nm.…”

Section: Cytotoxicity Analysismentioning

confidence: 99%

Effects of polyisoprenoids from Avicennia lanata and Avicennia alba leaves on the gene expression of PI3K, Akt1, mTOR, P53, and EGFR in human colorectal adenocarcinoma WiDr cells

et al. 2020

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Background: Mangrove plants produce polyisoprenoid compounds. Polyisoprenoids have been proven to have anticancer properties. This study investigated the inhibitory activity of polyisoprenoids derived from the leaves of mangrove plants Avicennia alba and Avicennia lanata regarding the expression of PI3K, Akt1, mTOR, P53, and EGFR genes against human colorectal adenocarcinoma WiDr cells. Methods: Anticancer activity was tested through the MTT assay method performed on WiDr cells. The cell cycle and apoptosis were analysed by flow cytometry and double staining. Gene expression of PI3K, Akt1, mTOR, P53, and EGFR was observed using the RT-PCR method. Results: Cytotoxic activity against WiDr cells showed that the IC50 for A. alba and A. lanata was 258.14 ug/mL and 243.32 ug/mL, respectively. This observation indicated the possibility to develop moderate anticancer agents. The cell cycle showed that inhibition of A. alba and A. lanata occurred in the late phase of apoptosis S (10.60 and 10.51%) and G2-M1 (22.05 and 23.84%), which was higher than negative and positive control cells. Furthermore, the polyisoprenoids derived from A. alba and A. lanata leaves exhibited anticancer activity in WiDr cells through the downregulated gene expression of PI3K, Akt1, mTOR, and EGFR as well as the upregulated gene expression of P53. Conclusion: This study demonstrated that polyisoprenoids obtained from A. alba and A. lanata leaves are promising chemopreventive agents for colon cancer.

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Cytotoxic Effect of Polyisoprenoids from Rhizophora mucronata and Ceriops tagal Leaves against WiDr Colon Cancer Cell Lines

Cited by 11 publications

References 35 publications

Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

Apoptotic with Double-Staining Test, P53, and Cyclooxygenase-2 to Proliferation Colon Cancer Cell (WiDr) of Dolichol in Three Mangrove Leaves

Current Status, Distribution, and Future Directions of Natural Products against Colorectal Cancer in Indonesia: A Systematic Review

Effects of polyisoprenoids from Avicennia lanata and Avicennia alba leaves on the gene expression of PI3K, Akt1, mTOR, P53, and EGFR in human colorectal adenocarcinoma WiDr cells

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