Cryopreservation of collared peccaries (Tayassu tajacu) semen using a powdered coconut water (ACP-116c) based extender plus various concentrations of egg yolk and glycerol

Abstract

The objective was to determine the effectiveness of a powdered coconut water-based extender (ACP-116c), plus various concentrations of egg-yolk and glycerol, as an alternative for cryopreservation of collared peccary semen. Twelve ejaculates were obtained from captive adult males by electroejaculation, and evaluated for sperm motility, kinetic rating, viability, morphology, and functional membrane integrity. The ejaculates were apportioned into aliquots that were diluted in Tris plus 10% egg yolk and 3% glycerol, or in ACP-116c plus 10 or 20% egg yolk and 1.5 or 3% glycerol. Samples were frozen in liquid nitrogen and, after 1 mo, thawed at 37 °C for 1 min. After thawing, samples were evaluated as reported for fresh semen, and also for sperm membrane integrity (fluorescent probes) and kinematic parameters (computerized analysis). Results were presented as means ± SEM. Freezing and thawing decreased sperm characteristics relative to fresh semen. Overall, ACP-116c plus 20% egg yolk and 3% glycerol provided better (P < 0.05) sperm motility and kinetic rating (48 ± 6.1% and 2.8 ± 0.2, respectively) after thawing than Tris extender (30.4 ± 5.7% and 2.4 ± 0.2). However, there were no differences (P > 0.05) among treatments with regard to the other sperm characteristics. Based on computerized motion analysis, total (26.5 ± 5.9%) and progressive (8.1 ± 2.2%) motility were best preserved (P < 0.05) with the above-mentioned treatment. In conclusion, a coconut water-based extender, ACP-116c, plus 20% egg yolk and 3% glycerol, was effective for cryopreservation of semen from collared peccaries.

Seminal coagulation and sperm quality in different social contexts in captive tufted capuchin monkeys (Sapajus apella)

“…Spermatic motility, vigor, sperm concentration and plasma membrane integrity were immediately evaluated after partial mechanical fragmentation. For this, the extender ACP‐118™ (powdered coconut water; ACP Biotecnologia, Fortaleza, Ceará, Brazil), which is commonly used as a seminal extender for Neotropical primates (Leão et al, ; Lima et al, ; Oliveira et al, , ,) and other mammals (Cardoso et al, ; Silva et al, , ; Uchoa et al, ) was added to each seminal coagulum (1:1) and maintained in a water bath (36°C) to allow coagulum liquefaction. Each coagulum was periodically (every 10 min) gently mixed with the help of a pipette tip in order to improve sample homogeneity (Oliveira et al, ).…”

Effect of Some Extenders on Chilled Rabbit Semen Stored at 5°c for 48 Hours

“…Coconut water-based extender, plus 20% egg yolk and 3% glycerol, was found to be effective for cryopreservation of semen from collared peccaries (Silva et al, 2012). Moreover, the inclusion of 80% citrate buffer in CME might be a suitable as buck semen extender for about 3 hours after extension of semen in room temperature (Sule et al, 2007).…”

<b>Induced reproduction of dourado (<i>Salminus brasiliensis</i>): fertilization with sperm cryopreserved in ACP^®-104

“…This product consists of a stable and standardized powder coconut water, which has suitable characteristics for use as an extracellular cryoprotectant (Silva et al, 2012).…”