<b>Induced reproduction of dourado (<i>Salminus brasiliensis</i>): fertilization with sperm cryopreserved in ACP^®-104

Abstract

ABSTRACT. Experiments were conducted to improve the fertilization rate of cryopreserved semen of dourado, Salminus brasiliensis. The experiment tested two cryoprotectant solutions at different semen: cryoprotectant ratios (1:5, 1:15, 1:25 and 1:50). The standard solution for the species (mixture of dimethyl sulfoxide, glucose, egg yolk and distilled water) was compared to a 350 mOsm ACP ® -104 solution, which is composed of powdered coconut water diluted in distilled water and methylglycol. Differences between the dilutions tested were significant only for ACP ® . The fertilization potential by using the standard solution at the lowest dilution (1:5) is equated when the sperm is diluted in ACP ® at 1:25 or 1:50. These results show that the standard solution is the most suitable for the cryopreservation of dourado sperm, since the dilution did not alter the fertilization rate, requiring smaller storage space. Os resultados apontam que a solução padrão é mais vantajosa para a criopreservação de sêmen do dourado, uma vez que a variação da diluição do sêmen não altera a taxa de fertilização, requerendo, assim, menor espaço para armazenamento das amostras.Palavras-chave: criopreservação, esperma, fertilização; crioprotetor, água de coco.

Spawning induction and embryonic development of Salminus hilarii (Characiformes: Characidae)

“…Water quality parameters observed in this study were similar to those observed in previous trials carried out with S. brasiliensis. In the same way, reproductive protocols used for this congeneric species were successfully applied for S. hilarii (Weingartner and Zaniboni-Filho, 2005;Zanandrea et al, 2016). In contrast, animals from the G2 group released a low amount of eggs and we failed to obtain fertilized eggs by manual striping.…”

Reproducción en cautiverio de vizcaína Curimata mivartii con extracto pituitario de carpa

“…Ante la ausencia de estímulos ambientales finales en cautiverio para la ovulación y el desove en los peces, uno de los métodos para superar esta disfunción reproductiva es la hipofización (Atencio, 2001), siendo el extracto pituitario de carpa (EPC) uno de los inductores más utilizados (Yaron et al, 2009). Existen registros de reproducción de otros Characiformes con importancia comercial con EPC como bocachico (Atencio-García et al, 2013), cachama blanca (Díaz-Olarte et al, 2010;Castillo-Losada et al, 2016), cachama negra (Arias Acuña y Hernández Rangel, 2009), dorada (Atencio-García et al, 2017), charúa (Atencio-García et al, 2006;Montes y Salgado 2014), comelón (Atencio-García et al, 2001), picuda Salminus affinis (Weingartner y Zaniboni-Filho, 2010;Zanandrea et al, 2016), sábalo Prochilodus lineatus (Botta et al, 2010); lo que señala la efectividad del EPC al permitir la reproducción inducida de especies nativas migratorias que no desovan en cautiverio. Son escasos los reportes en la literatura evaluando la reproducción inducida en especies de la familia Curimatidae; por lo que el objetivo del presente estudio fue evaluar la reproducción inducida de vizcaína Curimata mivartii con EPC.…”