Criopreservação do sêmen de curimba (Prochilodus lineatus) mediante adição de diferentes diluidores, ativadores e crioprotetores

Abstract

Amostras de sêmen de cinco espécimes de Prochilodus lineatus (Valencienes, 1847) foram utilizadas para avaliação dos efeitos tóxicos e crioprotetores de seis soluções à base de BTS (Beltsville Thawing Solution®) 4,5% enriquecidas com metanol e DMSO nas concentrações de 10% (A = BTS 4,5% + Metanol 10%; B = BTS 4,5% + DMSO 10%; C = BTS 4,5% + KCl 0,072% + metanol 10%; D = BTS 4,5% + KCl 0,072% + DMSO 10%; E = BTS 4,5% + KI 0,036% + metanol 10%; e F = BTS 4,5% + KI 0,036% + DMSO 10%). Foram avaliadas ainda três soluções ativadoras (água destilada, NaHCO3 60 mM e NaHCO3 119 mM), antes e após o congelamento. Estudaram-se a taxa e a duração da motilidade espermática. Não houve diferença significativa entre as soluções crioprotetoras utilizadas. O sêmen ativado por NaHCO3 60 e 119 mM apresentou as maiores taxas de motilidade espermática. A ativação por NaHCO3 119 mM possibilitou as maiores durações de motilidade no sêmen de P. lineatus.

Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis

“…The sperm motility values observed in this study after one month of freezing were higher than those obtained by SANCHEZ et al Activation of cryopreserved semen from dourado using an activator solution of 1% NaHCO 3 exhibited a motility time value similar to that activated with 0.45% NaCl, and both of these values were higher than water. This fact can be associated with a lower osmotic difference between activator to cytoplasm, preserving the integrity of the cytoplasmic membrane of spermatozoa (MURGAS et al, 2007). A similar result was observed for the activation of cryopreserved semen of Leporinus elongatus (MURGAS et al, 2007) and for Brycon insignis (VIVEIROS et al, 2011).…”

“…This fact can be associated with a lower osmotic difference between activator to cytoplasm, preserving the integrity of the cytoplasmic membrane of spermatozoa (MURGAS et al, 2007). A similar result was observed for the activation of cryopreserved semen of Leporinus elongatus (MURGAS et al, 2007) and for Brycon insignis (VIVEIROS et al, 2011). Nevertheless, CAROLSFELD et al (2003) have not found a difference between dourado semen activated with water or 1% NaHCO 3 .…”

Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae)

“…The initial and final doses of hormone given were 0.5 and 5 mg/kg of fish and 0.4 and 4 mg/kg of fish, for females and males respectively. The application of the hormone was done intramuscularly, with a syringe of 1 mL and near the base of the dorsal fin, at intervals of 12 h (Murgas et al, 1997;Ninhaus-Silveira et al, 2006).…”

“…They would not only benefit from programs to recover rivers, but also programmes for the reproduction of these species in captivity by artificial propagation and reintroduction (Murgas et al, 1997;Viveiros et al, 2010a). Cryopreservation of embryos is one alternative for the conservation of fish species as this technique allows maintenance of cells in liquid nitrogen for long periods and preserves genetic material.…”

Vitamin E and reduced glutathione in Prochilodus lineatus (curimba) semen cryopreservation (Characiformes: Prochilodontidae)

“…Cryopreservation of fish semen is a method that has been used to promote the preservation of endangered species (Murgas et al, 2007). The cryopreserved semen can be used in captive breeding programmes and for semen banks to ensure genetic diversity and reproductive success (Viveiros et al, 2009).…”