Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis

Weingartner, Marcos; Zanandrea, Ana Carolina Volpato; Zaniboni‐Filho, Evoy

doi:10.1590/0103-8478cr20140665

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The NaCl solution has been used to trigger sperm motility of fish from different orders, such as characiforms B. insignis (present study; Viveiros et al, ), P. lineatus (Viveiros, Orfão, Maria, & Allaman, ) and Colossoma macropomum (Oliveira et al, ), cypriniforms Barbus barbus (Alavi, Rodina, Policar, & Linhart, ), Puntius conchonius (Hu, Zhang, Zhou, & Zhang, ), Vimba vimba (Alavi, Kozak, Hatef, Hamackova, & Linhart, ), Clinostomus elongatus (Butts, Alavi, Mokdad, & Pitcher, ) and Cyprinus carpio (Khara et al, ), and acipenseriforms Acipenser ruthenus (Alavi, Gela, Rodina, & Linhart, ), just to name a few. The NaHCO 3 solution has been used to trigger sperm motility of characiforms species, such as B. insignis (Viveiros et al, ), Leporinus obtusidens (Taitson, Chami, & Godinho, ) and Salminus brasiliensis (Weingartner, Zanandrea, & Zaniboni, ). The use of activating solutions containing K + ion, such as KCl solution, to trigger fish sperm motility has been reported in cypriniforms B. barbus (Alavi, Rodina, Policar, et al, ), C. elongatus (Butts et al, ) and C. carpio (Khara et al, ) in characiforms, this is the first report.…”

Section: Discussionmentioning

confidence: 99%

Ions and osmolality on post‐thaw sperm motility activation of the endangered Brycon insignis (Characiformes)

et al. 2019

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Summary The aim of this study was to evaluate the effects of osmolality and the presence of ions on the activation of post‐thaw sperm motility of Brycon insignis. Sperm was frozen under a standardized methodology for this species. In experiment 1, 11 solutions were prepared with reverse osmosis (RO) water (∼0 mOsm/kg) and glucose or NaCl adjusted to an osmolality of 50, 100, 150, 200 and 250 mOsm/kg. In experiment 2, six solutions were prepared with RO and adjusted to ~100 mOsm/kg with one of the following chemicals: NaHCO3, sodium citrate (Na3C6H5O7), NaCl, KCl, CaCl2 or glucose (as ion‐free control). Post‐thaw sperm of both experiments was evaluated for motility rate, velocities (curvilinear = VCL, among others) and beat‐cross frequency (BCF). In experiment 1, sperm motility rate and velocities were higher (p < 0.05) when triggered in solutions at osmolalities from 0 to 200 mOsm/kg (62–80% motility; 139–167 µm/s) than that at 250 mOsm/kg (36–44% motility; 94–99 µm/s VCL). BCF was not affected by osmolality and varied from 19 to 24 Hz in all samples. In experiment 2, samples activated in NaHCO3, citrate, NaCl and KCl solutions yielded higher motility rates (76–85%) and BCF (24–25 Hz) compared to those activated in CaCl2 (50%; 14 Hz). Samples activated in ion‐free control solution yielded higher motility rate (87%) than those activated in NaHCO3 and in CaCl2. Curvilinear velocity was higher in samples activated in NaHCO3, citrate, KCl and control solutions (144–160 µm/s) than in those activated in CaCl2 (104 µm/s); samples activated in NaCl yielded intermediate VCL values (127 µm/s). Post‐thaw sperm achieves maximum motility rate and velocities when activated in solutions composed of sodium citrate, NaCl, KCl or glucose. Thus, post‐thaw sperm motility of B. insignis can be triggered in ionic and non‐ionic solutions at osmolality between 0 and 200 mOsm/kg. The use of solutions containing calcium, however, should be avoided.

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Section: Discussionmentioning

confidence: 99%

Ions and osmolality on post‐thaw sperm motility activation of the endangered Brycon insignis (Characiformes)

et al. 2019

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“…Cuando la seminación se realiza con semen crioconservado, la relación semen:ovocitos deben ser aún mayor, dado que la congelación provoca una alta mortalidad de espermatozoides y disminución de la viabilidad celular . Para semen crioconservado se reporta una relación espermatozoides/ovocitos de 3.5x10 4 para Salminus brasiliensis (Weingartner et al, 2015); 1x10 5 para Salvelinus fontinalis, Oncorhynchus mykiss y Cyprinus carpius (Lahnsteiner et al, 2011;Öğretmen et al, 2014;Kutluyer et al, 2015); 1.6x10 5 para Sorubim cuspicaudus (Pardo-Carrasco et al, 2015); 2.5x10 5 para Colossoma macropomum (Maria et al, 2015); 2.7x10 5 para Salminus brasiliensis ; 3.2x10 5 para Prochilodus magdalenae (Atencio-García et al, 2015); 2.7x10 7 para Perca flavescens (Miller et al, 2018); 3x10 5 para Salmo trutta y Salvelinus fontinalis (Nynca et al, 2014(Nynca et al, , 2015b; 5x10 4 para Thymallus thymallus (Horváth et al, 2015); 5x10 4 -4x10 5 para Esox lucius (Irena et al, 2020); 5x10 4 -5x10 5 para Perca fluviatilis (Judycka et al, 2019); 5x10 5 para Hucho hucho (Nynca, 2015c); 5,15×10 5 para Piaractus mesopotamicus (Galo et al, 2019); 6x10 6 para trucha arco iris (Ciereszko et al, 2014); 7×10 4 para Prochilodus lineatus ; 7.5 ×10 4 para Brycon amazonicus (Velasco-Santamaría et al, 2006).…”

Section: Fertilidadunclassified

Crioconservación seminal en peces de agua dulce: aspectos biotecnológicos, celulares y bioquímicos

2020

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La crioconservación es una herramienta biotecnológica que en peces está orientada principalmente a la conservación criogénica de semen como estrategia de preservación del recurso genético y a su uso para la producción de alevinos con fines diferentes. Actualmente, los protocolos de crioconservación seminal en peces de agua dulce establecen una amplia variedad de procedimientos cuya efectividad se basa en aspectos ligados a la calidad seminal post-descongelación y la fertilidad, así como su relación con el desarrollo de la progenie. El efecto de la conservación del semen en nitrógeno líquido por periodos amplios de tiempo también toma importancia en ésta biotecnología. Por lo anterior, el objetivo de la presente revisión es describir aspectos biotecnológicos, celulares y bioquímicos asociados al proceso de crioconservación seminal en peces dulceacuícolas, resaltando los avances, las limitaciones y sus perspectivas.

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“…Cryopreservation is a technique that can provide good quality spermatozoa for artificial insemination (4). Cryopreservation is used in fish biotechnology in order to solve the cost, risk in fish transport, limited broodstock, gamete storage and also spermatozoa banking (5,6). Thus, cryopreservation can be used in Sutchi Catfish to provide spermatozoa from male broodstock for breeding activity continuously.…”

Section: Introductionmentioning

confidence: 99%

Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

Fanni¹,

Santanumurti²,

Suprayogi³

et al. 2019

IJVS

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Sutchi Catfish is one of the important fish commodities in Indonesia. Unfortunately, its seasonal spawning pattern causes limited supply. Cryopreservation is a solution to solve limited supply since it can store the spermatozoa in low temperature so that physiological, biological and morphological functions still remain. Improving the quality of cryopreservation is important to increase the success of Sutchi Catfish aquaculture. Adding honey in cryopreservation process is expected to increase the quality of spermatozoa since it contains with sugars as a source of spermatozoa's energy. This study tried to compare the effectivity of honey in cryopreservation process with no addition. The treatments used in this study were T1 (0% honey), T2 (0.2% of honey), T3 (0.4% of honey), T4 (0.6% of honey) and T5 (0.8% of honey). 30 days after stored, the spermatozoa were checked their motility, viability, abnormality, fertility and hatching rate. This study showed that honey addition could increase the motility significantly (P<0.01) to 23.14% better than control. The viability increased significantly (P<0.01) to 23.17% better than control. The abnormality test did not show significant difference between honey addition and control although the abnormality value in control was the highest (10.75%). The fertilization rate increased significantly (P<0.01) to 28.85% better than control. The hatching rate increased significantly (P<0.01) to 29.78% better than control. The success of all test indicated that the addition of honey in cryopreservation process of spermatozoa could be performed on Sutchi Catfish to increase its production even though the limited spawning pattern.

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Cryopreserved sperm for oocyte fertilization of dourado Salminus brasiliensis

Abstract: Salminus brasiliensis is a migratory fish that has

Cited by 3 publications

References 17 publications

Ions and osmolality on post‐thaw sperm motility activation of the endangered Brycon insignis (Characiformes)

Ions and osmolality on post‐thaw sperm motility activation of the endangered Brycon insignis (Characiformes)

Crioconservación seminal en peces de agua dulce: aspectos biotecnológicos, celulares y bioquímicos

Quality enhancement of cryopreserved spermatozoa of sutchi catfish (Pangasianodon hypophthalmus) with honey addition

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