Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

Stegani, Vanessa; Alves, Guilherme Augusto Cito; Bertoncelli, Douglas Junior; Faria, Ricardo Tadeu de

doi:10.14295/oh.v23i1.921

Cited by 4 publications

(1 citation statement)

References 9 publications

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“…O teor inicial de água é fundamental para a criopreservação e garantir a sobrevivência das sementes após serem retiradas do nitrogênio líquido (Stegani et al 2017). Recomenda-se que o mesmo esteja abaixo de 10 % antes de congelar o material (Silva et al 2011), valor este atendido neste estudo.…”

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Criopreservação de sementes de espécies brasileiras do gênero Dyckia (Bromeliaceae)

et al. 2022

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RESUMO -Objetivou-se avaliar diferentes crioprotetores na criopreservação em nitrogênio líquido (NL) de sementes das bromélias Dyckia dusenii, Dyckia kranziana e Dyckia walteriana e quantificar o teor de lipídios das sementes. Os tratamentos foram: controle, imersão em NL, glicerol 1 mol.L -1 , sacarose 0,4 mol.L -1 , PVS1, PVS2, PVS2 + 1 % floroglucinol e PVS3. Das sementes foi avaliado o teor total de lipídios. Avaliaram-se o percentual de germinação e plântulas anormais, índice de velocidade de germinação, comprimento e massa seca das plântulas. Após 150 dias, o comprimento e massa seca da parte aérea e radicular. Os crioprotetores não foram eficazes nas sementes de D. dusenii e D. kranziana, no entanto, para as de D. walteriana é recomendado o uso dos crioprotetores glicerol 1 mol.L -1 , sacarose 0,4 mol.L -1 , PVS1, PVS2, PVS2 + 1 % floroglucinol ou PVS3. Estudos da composição dos ácidos graxos saturados e insaturados são necessários para estabelecer uma relação com a criopreservação.Palavras-chave: banco de germoplasma, Dyckia dusenii, Dyckia kranziana, Dyckia walteriana, nitrogênio líquido. ABSTRACT -Cryopreservation of seeds of Brazilian species of the genus Dyckia (Bromeliaceae).The objective of this study was to evaluate different cryoprotectants in cryopreservation in liquid nitrogen (NL) of seeds of Dyckia dusenii, Dyckia kranziana and Dyckia walteriana and to quantify the lipid content of the seeds. The treatments were: control, immersion in NL, glycerol 1 mol.L -1 , sucrose 0.4 mol.L -1 , PVS1, PVS2, PVS2 + 1% floroglucinol and PVS3. From the seeds, the total lipid content was evaluated. The percentage of germination and abnormal seedlings, germination speed index, length and dry mass of the seedlings were evaluated. After 150 days, the length and dry mass of the aerial and root parts. The cryoprotectants were not effective in the seeds of D. dusenii and D. kranziana, however, for those of D. walteriana it is recommended to use the cryoprotectants glycerol 1 mol.L -1 , sucrose 0.4 mol.L -1 , PVS1, PVS2 , PVS2 + 1 % floroglucinol or PVS3. Studies of the composition of saturated and unsaturated fatty acids are necessary to establish a relationship with cryopreservation.

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Section: Resultsunclassified

Criopreservação de sementes de espécies brasileiras do gênero Dyckia (Bromeliaceae)

et al. 2022

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Efficacy of Passion Fruit Cryopreservation Using Cryopotectant Agents

Faria

Luz

Sobrino

et al. 2020

International Journal of Fruit Science

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CRYOPRESERVATION OF SEEDS OF THE BRAZILIAN NATIVE SPECIES AROEIRA-DO-SERTÃO (Astronium urundeuva M. Allemão Engl.)1

et al. 2022

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Aroeira-do-sertão is a Brazilian native species that has been widely explored. Thus, the population of this species has been reduced and techniques for its preservation are essential, such as the conservation of seeds in liquid nitrogen (LN). The objective of this work was to evaluate different cryoprotectant solutions for cryopreservation of aroeira-do-sertão seeds in LN (-196 °C). The treatments used were: control (7.5±1.5 °C); LN without cryoprotectant; sucrose 0.4 mol L-1; sucrose 0.8 mol L-1; glycerol 1 mol L-1; glycerol 2 mol L-1; PVS1 (plant vitrification solution); PVS2; PVS2 + 1% phloroglucinol; and PVS3. The seeds remained frozen for 120 days. The seeds were evaluated for germination and water content before cryopreservation. Several germination parameters were evaluated on the seventh day and plant growth variables were evaluated after 150 days. The seeds presented 9.2% water content and 74% germination before cryopreservation. The germination in the control treatment was 55%, whereas it varied from 61% to 69% under cryopreservation, denoting the positive effect of cryopreservation, even without cryoprotectants (69%). The seeds presented a triphasic water absorption model; the LN accelerated the germination, which started within 56 hours, whereas the germination in the control treatment started after 66 hours. The plant parts presented satisfactory development after 150 days, as shown by the Dickson quality index. The use of cryoprotectants did not affect seed germination and initial growth of seedlings. Aroeira-do-sertão seeds with 9.2% water content can be cryopreserved in LN without cryoprotectants.

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Criopreservação de sementes de rainha do abismo (Sinningia leucotricha)

Cited by 4 publications

References 9 publications

Criopreservação de sementes de espécies brasileiras do gênero Dyckia (Bromeliaceae)

Criopreservação de sementes de espécies brasileiras do gênero Dyckia (Bromeliaceae)

Efficacy of Passion Fruit Cryopreservation Using Cryopotectant Agents

CRYOPRESERVATION OF SEEDS OF THE BRAZILIAN NATIVE SPECIES AROEIRA-DO-SERTÃO (Astronium urundeuva M. Allemão Engl.)1

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