Efficacy of Passion Fruit Cryopreservation Using Cryopotectant Agents

Faria, Ariane de; Luz, Petterson Baptista da; Sobrino, Severino de Paiva; Medeiros, Carolina Moreira de; Tavares, Armando Reis; Soares, Fabiano Silva

doi:10.1080/15538362.2020.1753139

Cited by 5 publications

(1 citation statement)

References 22 publications

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“…This generally involves using liquid nitrogen at −196°C, because at that temperature, cell division, and other metabolic processes are virtually halted, so the material can remain stored indefinitely without undergoing structural alterations (Goldfarb et al, 2010). For Passiflora , several studies have been conducted regarding cryopreservation (Araújo et al, 2016; Faria et al, 2020; Garcia et al, 2011; Generoso et al, 2019; González‐Benito et al, 2009; Merhy et al, 2014; Veiga‐Barbosa et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Seed morphoanatomy of the genus PassifloraL. (Passifloraceae) by scanning electron microscopy and light microscopy

Silva

Souza

Junghans

et al. 2022

Microscopy Res & Technique

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Morphoanatomical analysis of seeds contributes to knowledge of the development of seedlings and identification of species, as well as supporting conservation studies. The conservation of the species belonging to the Passiflora genus is crucial due to of the threats to the genetic resources of these species. Thus, the objective of this study was to morphoanatomically characterize Passiflora seeds, verify possible injuries to the tissues after cryopreservation and thus contribute to the conservation strategies of the species of this genus. Initially, seeds of Passiflora coccinea, P. edulis, P. gibertii, P. maliformis, P. morifolia, P. setacea, P. suberosa, and P. tenuifila collected from the Passion Fruit Active Germplasm Bank of the Embrapa Cassava and Fruits research unit (Embrapa Mandioca e Fruticultura) were analyzed. Then, their length, width and thickness, shape of the base and tip, and ornamentations present on the body and edge of the seeds were evaluated. The seeds of the species were placed in cryotubes and immersed in liquid nitrogen to assess possible cryoinjuries. The tegument and tissues of the seeds were examined by scanning electron microscopy. The seeds had varied biometric data, with average values of 4.63 mm for length, 3.28 mm for width, and 1.51 mm for thickness. Six ornamentation types were observed: reticulate for the species P. coccinea; finely reticulate for P. edulis; foveolate reticulate for P. gibertii and P. setacea; alveolate reticulate for P. maliformis and P. tenuifila; coarsely reticulate for P. morifolia; and falsifoveolate reticulate for P. suberosa. Some seeds suffered tegument cracks due to the freezing in liquid nitrogen, but without physiological damages to the embryo and endosperm. The cryopreservation of the seeds in the presence of the tegument significantly reduced the cryoinjuries caused to the embryo. Cryopreservation can be promising for long-term conservation of passion fruit seeds.

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