Conversion of nitrite to nitric oxide at zinc via S-nitrosothiols

Abstract

Nitrite is an important reservoir of nitric oxide activity in the plasma and cells. Using a biomimetic model, we demonstrate the conversion of zinc-bound nitrite in the tris(pyrazolyl)borate complex (iPr2)TpZn(NO2) to the corresponding S-nitrosothiol RSNO and zinc thiolate (iPr2)TpZn-SR via reaction with thiols H-SR. Decomposition of the S-nitrosothiol formed releases nitric oxide gas.

Effect of Cimetidine on Nitro-Oxidative Stress in a Rat Model of Periodontitis

“…Nitrite can be reduced to NO by hypoxia, tissue acidosis, or by enzymes. These phenomena make serum levels of nitrates indicators of NO production in vivo and important complementary reservoirs of NO in physiological conditions [ 29 ].…”

Crystal-facet-dependent denitrosylation: modulation of NO release fromS-nitrosothiols by Cu2O polymorphs

“…The title compound was synthesized following a reported procedure with minor modifications 13. Briefly, FeSO 4 ·7H 2 O (217 mg, 0.78 mmol) and diethylammonium diethyldithiocarbamate (347 mg, 1.56 mmol) were added in dichloromethane (10 mL) and the reaction mixture was stirred for 8 h. The resultant brown solution was filtered, and the brown filtrate was dried under reduced pressure to obtain (dtc) 2 Fe II as a brown solid, which was then recrystallized in diethyl ether to get pure (dtc) 2 Fe II .…”

Effect on health from consumption of meat and meat products

“…Many studies have shown that nitrite ions are physiologically recirculated in blood and tissues through the process of reduction or oxidation under the influence of hemoglobin and myoglobin, producing various nitrogen oxides [46,[88][89][90][91]. Nitrite ions can be reduced in the body to produce NO or maintain nitrosothiols to maintain the constancy of NO [92] and, nitrite ions are the main factors that maintain NO constancy in the body [17].…”