Contamination of Escherichia coli O157:H7 in dairy cow farms

Abstract

<em>E. coli</em> serotype O157:H7 is a foodborne pathogen that endangers human health. Cattle are the main reservoir of this bacterium. E. coli O157:H7 that come out of the livestock can contaminate the surrounding environment, dairy products, meat, vegetable or fruit crops, so it can act as a source of infection and disease in humans. This research was conducted to identify the contamination of <em>E. coli</em> O157:H7 on dairy cow farms so the current situation is known. Sampling area for this research was dairy cow farm in Depok, Cianjur, Sukabumi, and Bandung. Types of samples taken are cow anal swab or fresh feces and livestock drinking water. Isolation of <em>E. coli</em> bacteria was done on mTSB media, agar MacConkey, and Eosin Methylene Agar. Identification of bacteria as E. coli O157:H7 was done by biochemical test and for serotype determination was used agglutination test using monospesifik antiserum for O157 and H7. The results of research showed that of the total 178 samples had 126 (70.8%) samples containing <em>E. coli</em> bacteria. Of the 126 samples containing <em>E. coli</em> bacteria, 94 (74.6%) were determined as E. coli serotype O157:H7. The presence of <em>E. coli</em> O157:H7 contaminants on dairy cow farms, their potential impacts on human health, prevention and control of the disease is discussed in this paper.

Escherichia coli O157:H7 Resistan Antibiotik Pada Daging Kebab yang Dijual di Sekitar Kampus IPB Dramaga Bogor

“…Uji konfirmasi E. coli O157 dilakukan dengan uji aglutinasi latex (latex agglutination). Menurut Rachmawati & Ariyanti (2017) hasil positif O157 ditandai dengan terbentuknya aglutinasi seperti endapan pasir dalam waktu 1-3 menit.…”