This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.
Kebab merupakan salah satu makanan siap saji atau ready to eat (RTE) yang populer di seluruh dunia. Escherichia coli (E. coli) O157:H7 banyak dihubungkan dengan kejadian outbreak foodborne disease pada kebab. Kontaminasi E. coli O157:H7 resistan antibiotik pada kebab dapat menimbulkan masalah kesehatan serius. Penelitian ini bertujuan mengidentifikasi E. coli O157:H7 resistan antibiotik yang diisolasi dari daging kebab yang dijual di sekitar Kampus IPB Dramaga Bogor. Total 43 sampel daging kebab diambil dari seluruh pedagang kebab di sekitar Kampus IPB Dramaga dalam radius 2 km dari batas terluar Kampus. Isolasi dan identifikasi E. coli mengacu pada Standar Nasional Indonesia (SNI) 2897:2008 dari Badan Standardisasi Nasional tentang Metode Pengujian Cemaran Mikroba dalam Daging, Telur, dan Susu, serta Hasil Olahannya. Uji serotyping E. coli O157:H7 menggunakan uji Serologis. Uji resistansi E. coli O157:H7 mengacu pada standar Clinical Laboratory Standards Institute (CLSI) dan dilakukan terhadap 10 jenis antibiotik menggunakan metode Kirby-Bauer disk diffusion. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan enam isolat positif E. coli O157:H7 (31.6%; 6/19) yang resistan terhadap ampisilin, amoksisilin-asam klavulanat, sefotaksim, gentamisin, siprofloksasin, enrofloksasin, kolistin sulfat dengan satu isolat termasuk multidrug resistant (MDR). Semua isolat E. coli O157:H7 masih sensitif terhadap trimethoprim-sulfametoksasol, oksitetrasiklin, dan kloramfenikol.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.