Antibiotic Resistance in Escherichia coli Isolated from Döner Kebab Sold in Dramaga Bogor, Indonesia

Abstract

This study was aimed to determine the occurrence of antibiotic resistance in Escherichia coli (E. coli) isolated from Döner kebab sold in Dramaga Bogor, West Java Province, Indonesia. A total of 43 samples of Dὂner kebab meat were taken from all of the vendors in the radius of 2 km around the border of IPB University campus, Dramaga Bogor. Isolation and identification of E. coli were referred to the Guideline for Laboratory Analysis on Examination of Microbial Contamination in Meat, Egg, and Milk in SNI 2897:2008 issued by the National Standardization Agency of Indonesia. The resistance against nine antibiotics were tested using Kirby-Bauer disk diffusion method based on the standard of Clinical Laboratory Standards Institute (CLSI) in 2018. The result showed that nine samples were positive containing E. coli (20.9%) and 84.2% of E. coli isolates were resistant to eight antibiotics: gentamicin (57.9%), ampicillin (26.3%), cefotaxime, trimethoprim-sulfamethoxazole, and ciprofloxacin (21.1%), amoxicillin-clavulanate (10.5%), enrofloxacin, and oxytetracycline (5.3%). All of E. coli isolates were susceptible to chloramphenicol (100%). There were 21.1% of isolates also resistant to three or more classes of antibiotics that were known as multi-drug resistant (MDR) with different resistant patterns.The presence of E. coli resistant antibiotic in kebab meat could results serious problems in human health.

Resistansi Penisilin terhadap Escherichia coli pada Susu Segar yang berasal dari Koperasi Ternak Sapi Cianjur Utara (KPSCU), Jawa Barat

“…Konsumsi susu yang mengandung residu antibiotik dapat menimbulkan gejala diare, keracunan maupun gejala alergi (Detha, 2014). Mikroba dapat masuk kedalam susu segar secara langsung dari sapi perah, dari lingkungan peternakan, sumber air dan peralatan yang digunakan untuk penyimpanan susu di peternakan atau selama transportasi (Ravi, 2020).…”

“…al. lembaga ini melaporkan bahwa prevalensi antibiotika di wilayah Jawa Barat cukup tinggi, terutama Bogor yaitu 3.06% untuk penisilin, 28.57% untuk makrolida, 2.55% untuk aminoglikosida, dan 47.45% untuk tetrasiklin. Bakteri E. coli resistan terhadap antibiotik penisilin (95.2%), streptomisin (84,3%), kolistin (76.9%), amoksilin (74.2%) dan tetrasiklin (62.5%) (Ravi 2020). Antimikroba yang sering digunakan dalam pengobatan penyakit pada sapi perah menurut Page dan Gautier (2012) adalah penisilin, streptomisin dan sefalosporin.…”

Antimicrobial resistance in <i>Escherichia coli</i> isolated from brown rats and house shrews in markets, Bogor, Indonesia

“…Moreover, ESBL-producing E. coli was found in 8.6% of cattle feces from slaughterhouses [ 26 ], and multi-drug resistant (MDR) E. coli was reported in 57.3% of swine fecal samples [ 14 ]. MDR among AMR E. coli was also found in 25% from Döner Kebab meat in Bogor, Indonesia [ 23 ]. These results indicated that a variety of AMR phenotypes in E. coli were existed and might be a widespread in the environment, Indonesia.…”