Confocal Raman microscopy of pathologic cells in cerebrospinal fluid

Abstract

In this work, the spatial localization of leucocytes, bacteria, and erythrocytes in the crystal pattern of a dried droplet of cerebrospinal fluid (CSF) is established. Characteristic lines are detected and identified in the Raman spectrum of the CSF that point to the presence of pathologic cells therein and can be used in a timely way to diagnose meningitis, the spectroscopic sample preparation procedure being simple enough. A dry CSF sample retains its characteristic spectral features for no less than three days, which is important for its safe keeping and transportation, and also for the computer processing of its spectra.

The application of Raman spectroscopy for the detection and identification of microorganisms

“…The primary advantages of the proposed methodology are a high degree of intrinsic specificity without necessitating substantive sample preparation and the ability to perform direct measurements on the clinical samples without prolonged microbiologic culture . In the work of Gonchukov et al ., the spatial localization of leukocytes, bacteria, and erythrocytes in the crystal pattern of a dried droplet of CSF was established . A simple sample preparation was performed, and characteristic patterns in the Raman spectra of the CSF were detected and identified, which point to the presence of pathologic cells.…”

“…[150] In the work of Gonchukov et al, the spatial localization of leukocytes, bacteria, and erythrocytes in the crystal pattern of a dried droplet of CSF was established. [151] A simple sample preparation was performed, and characteristic patterns in the Raman spectra of the CSF were detected and identified, which point to the presence of pathologic cells.…”

Principles and applications of Raman spectroscopy in pharmaceutical drug discovery and development

“…Diem et al discussed the use of Raman spectroscopy for detection of metastases in lymph nodes, squamous cell carcinoma, adenocarcinomas, brain tumors and brain metastases by studying the histopathology of tissues [112]. Gonchukov et al developed a method using confocal Raman microscopy for diagnosis of meningitis by detecting spatial localization of leucocytes, bacteria and erythrocytes in cerebrospinal fluid [113].…”

Diagnosis of chronic kidney diseases based on surface-enhanced Raman spectroscopy and multivariate analysis

“…The fingerprint spectral characteristic makes Raman spectroscopy a promising label-free and non-invasive diagnosis method as it can assesse the chemical constituents such as proteins, nucleic acids, carbohydrates in biological samples. Recently, Raman spectroscopy has been applied to detection of virus, identification of bacteria, analysis of cells, and pathological diagnoses [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. In particular, blood plasma or serum is a metabolite pool derived from nearly all the organs and tissues of the human body.…”