In this work, the spatial localization of leucocytes, bacteria, and erythrocytes in the crystal pattern of a dried droplet of cerebrospinal fluid (CSF) is established. Characteristic lines are detected and identified in the Raman spectrum of the CSF that point to the presence of pathologic cells therein and can be used in a timely way to diagnose meningitis, the spectroscopic sample preparation procedure being simple enough. A dry CSF sample retains its characteristic spectral features for no less than three days, which is important for its safe keeping and transportation, and also for the computer processing of its spectra.

In this work it is shown, that laser with injection of reflected radiation can possess the high longitudinal spatial selectivity. Laser figures here both the emitter and the detector of radiation. This attribute, having volumetric character for laser fields, can be considered as a new modality in optical microscopy. Using the experimental possibilities we achieved the spatial resolution at a level of several microns. The optimization of laser sources is to lead to longitudinal spatial resolution at a level of nanometers. (© 2012 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA) (© 2011 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA)