Principles and applications of Raman spectroscopy in pharmaceutical drug discovery and development

Abstract

Raman spectroscopy has a number of potential applications within drug discovery and development. It can be used to estimate the molecular activity of drugs and to establish a drug's physicochemical properties such as its partition coefficient. It can also be used in compatibility studies during the drug formulation process. Raman spectroscopy's immense potential should be further investigated in future.

Monitoring doxorubicin cellular uptake and trafficking using in vitro Raman microspectroscopy: short and long time exposure effects on lung cancer cell lines

“…To this end, Raman micro spectroscopy, a non-invasive powerful label-free in vitro screening tool, is presented as the ideal candidate due to its ability to detect chemical, biological and physical changes of biomolecules at subcellular level and provide a molecular fingerprint of the sample of interest, in this case cancer cell physiology, at a subcellular level, during chemotherapeutic treatment. (21)(22)(23)(24) Raman micro spectroscopy has attracted considerable attention over the last few years in the pharmaceutical field to aid in areas such as the drug discovery process, quality control during industrial manufacturing and detection of counterfeit products, (23,25,26) and well as its possible clinical applications. (27,28) Its potential as a screening technique either for normal and cancer cell discrimination or in chemotherapeutic and cellular process screening has been demonstrated.…”

Micro and nano liposome vesicles containing curcumin for a drug delivery system

“…They were observed at 1601 cm −1 (aromatic C=C) and 1626 cm −1 (carbonyl C=O). The peak at 1430 cm −1 was the characteristic peak of phenol C-O [24][25][26]. While two forms of curcumin structures are defined by the peak at 1249-1250 cm −1 , the keto-enol form of curcumin was obtained at 1250 cm −1 in the spectrum.…”

“…The strong peak between 1600 and 1630 cm −1 was attributed to the mixed ν(C=C) and ν(C=O) vibration mode [24][25][26]. They were observed at 1601 cm −1 (aromatic C=C) and 1626 cm −1 (carbonyl C=O).…”

Physicochemical properties of some tetrahydropyrimidine derivatives

“…As HCl is present in gastric juice in stomach, 0.1 N HCl was taken for the reaction. Blood [45] has 7.4 pH, so the study was done at pH 7.4. Further, the middle and upper range of body pH is 6.0 and 8.0 respectively, so study was also done at all of these pH values.…”

“…Partition coefficient is the most crucial parameter of QSAR and QSPR study [38][39]. The knowledge of solubility and the octanol-water partition coefficient of drug is important in drug discovery, development, and production [40][41][42][43][44][45]. Implementation of latest technology makes this research area easy and versatile.…”