Concentração dos sais e da sacarose do meio MS na multiplicação in vitro e na aclimatização de batata

Bandinelli, Maurício Guerra; Bisognin, Dílson Antônio; Gnocato, Francisco Saccol; Mambrin, Ritieli Baptista; Sausen, Darlene; Nicoloso, Fernando Teixeira

doi:10.1590/s0102-05362013000200011

Cited by 17 publications

(16 citation statements)

References 20 publications

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“…The clonal mini-hedge was established in polyethylene trays, placed in a greenhouse and fertirrigated as described in BANDINELLI et al (2013) For clonal mini-hedge formation, oneyear-old seedlings (seminal origin) and plantlets (rooted cuttings) were transplanted to the growing beds of the polyethylene trays. After 10 days of cultivation, they were pruned at 6.0cm height to get the ministumps, which provided the sprouts to prepare the mini-cuttings.…”

Section: O Objetivo Deste Trabalho Foi Avaliar a Produtividade De Minmentioning

confidence: 99%

Production and rooting of cordia - Cordia trichotoma (Vell.) Arrab. ex Steud. mini-cuttings collected from ministumps of asexual and seminal origin

et al. 2015

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This study aimed to evaluate the production of cordia (Cordia trichotoma) ministumps of seminal and asexual origin, as well as the effect of the naphthaleneacetic acid (NAA) on the rooting of mini-cutitings. Seedlings and plantlets from rootedIn clonal mini-hedge, the production of ministumps should vary among clones, management techniques, ministump age and vigor, among others factors (DIAS et al., 2012). The nutritional management has also been a determinant factor for the competency of mini-cutting rooting, because well-nourished propagules have high availability of endogenous auxin, amino acids, carbohydrates, and other substances that facilitate differentiation and root formation.Studies have shown that cordia (Cordia trichotoma), a native tree species of the Boraginaceae family, presents propagules with low-rooting competence, because unsatisfying rhizogenesis responses have been obtained in both mini-cuttings of adult shoots and cuttings (FICK, 2007;HEBERLE et al., 2010). As far as it is know, there are no references on cordia mini-cutting technology, which is the -NOTE -I

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Section: O Objetivo Deste Trabalho Foi Avaliar a Produtividade De Minmentioning

confidence: 99%

Production and rooting of cordia - Cordia trichotoma (Vell.) Arrab. ex Steud. mini-cuttings collected from ministumps of asexual and seminal origin

et al. 2015

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“…It is important to emphasize that these results corroborate the present study for the same evaluated characteristics, showing that plants from the Zingiberaceae family tend to respond positively to an increase in sucrose concentration in the culture medium. Different results were obtained for two species of S. tuberosum, which increase in sucrose concentration limited the number of leaves and the length of the aerial part of in vitro seedlings (Bandinelli et al, 2013). In Orbignya oleifera embryos, the interaction between sucrose and activated charcoal was not significant.…”

Section: Effect Of Activated Charcoal and Sucrosementioning

confidence: 78%

“…Moreover, some species such as Ocimum basilicum are frequently intolerant to high salt concentrations in the range of (70 to 100%) (Tarchoune et al, 2012). Species with concentration lower than 70% have been more favorable to the development and with regard to Solanum tuberosum where 50% of the MS medium salts caused vigorous and health seedlings (Bandinelli et al, 2013). As reported by Islam et al (2004), a 75% salt concentration was more important for induction of microrhizomes in turmeric than was treatment with 100% salt concentration.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Ferrari¹,

Antoniazzi²,

Nascimento³

et al. 2016

J. Med. Plants Res.

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Turmeric (Curcuma longa) is an Asian native species used for ornamental, medicinal and food purposes. However, its conventional propagation method is costly and inefficient. Therefore, this study aimed to verify appropriate asepsis methods for shoots from rhizomes, evaluate concentrations of activated charcoal and sucrose, and determine appropriate salt concentrations in Murashige and Skoog (MS) medium for turmeric growth. Three independent assays were performed. First, asepsis methods were verified for explants; second was for concentrations of sucrose and activated charcoal and in the final assay salt concentrations was evaluated. In all assays, MS medium supplemented with 30 g/L of sucrose, 6.5 g/L of agar and pH adjusted to 5.8 was used. Two growth regulators were added to the culture medium: 4.44 µM of 6-benzyl-amino-purine (BAP) and 1.08 µM of α-naphthalene acetic acid (NAA). Data obtained were subjected to analysis of variance (ANOVA) at p ≤ 0.05 probability. The averages were compared by Tukey's test and polynomial regression at p ≤ 0.05. All the asepsis methods evaluated showed similar effects. The highest concentration was obtained by utilizing 60 mg/L sucrose for the most part of the evaluated characteristics; however, it is recommended that activated charcoal be used at a concentration of 4.5 g/L. For salt concentrations, averages were compared by polynomial regression at (p ≤ 0.05) probability in 50 to 60% MS medium, and resulted in longer root systems, greater numbers of shoots and more leaves. The usual dose of 3% sucrose resulted in lower development of in vitro seedlings. Salt concentrations higher than 60% were toxic to turmeric tissues and consequently compromised the root system and the aerial part of developing seedlings.

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“…In potato plants cultured in vitro, Bandinelli et al (2013) observed that the increase of sucrose concentration in the MS medium was accompanied by a decrease in the biomass production. The dynamics of solute translocation in the phloem helps to explain the reduction in the height of seedlings cultured in medium with higher concentrations of sucrose.…”

Section: Resultsmentioning

confidence: 99%

Effect of seed desiccation and sucrose concentration on the in vitro establishment of mangabeira (Hancornia speciosa Gomes var. gardneri) seedlings

Santos¹,

Aguiar²,

Brandão³

et al. 2017

Afr. J. Agric. Res.

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Mangabeira (Hancornia speciosaGomes) and four natural drying periods (24, 48, 96 and 192 h after the seed pulping) were used in a factorial experiment. 60 days after in vitro culturing of the naked embryos, the following variables were evaluated: germination %, germination speed index (GSI), mean germination time (MGT), oxidation index, culture medium contamination index, length of the largest root, stem diameter, seedling height and number of live and dead leaves. The interaction between desiccation times and sucrose concentrations was not significant for any of the variables analyzed. Germination was influenced only by the desiccation time, being reduced after 106 hours of natural drying. Increasing concentrations of sucrose up to 60 g L -1 reduced GSI and seedling height. In contrast, leaf mortality decreased, which contributed to the production of seedlings with greater ability of acclimatization to field conditions.

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Concentração dos sais e da sacarose do meio MS na multiplicação in vitro e na aclimatização de batata

Abstract: Palavras-chave: Solanum tuberosum, sobrevivência ex vitro, produção de mudas, propagação vegetativa. ABSTRACTMS salt and sucrose concentrations in the in vitro multiplication and acclimatization of potato

Cited by 17 publications

References 20 publications

Production and rooting of cordia - Cordia trichotoma (Vell.) Arrab. ex Steud. mini-cuttings collected from ministumps of asexual and seminal origin

Production and rooting of cordia - Cordia trichotoma (Vell.) Arrab. ex Steud. mini-cuttings collected from ministumps of asexual and seminal origin

Evaluation of new protocols to Curcuma longa micropropagation: A medicinal and ornamental specie

Effect of seed desiccation and sucrose concentration on the in vitro establishment of mangabeira (Hancornia speciosa Gomes var. gardneri) seedlings

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