Complete Genome Sequence of Multidrug-Resistant Citrobacter freundii Strain P10159, Isolated from Urine Samples from a Patient with Esophageal Carcinoma

Liu, Xiaodong; Huang, Yong; Xu, Xiaomeng; Zhao, Yachao; Sun, Qiang; Zhang, Zhiyi; Zhang, Xianglilan; Wu, Yi; Wang, Jie; Zhou, Dongsheng; An, Xiaoping; Pei, Guangqian; Wang, Yunfei; Mi, Zhiqiang; Yin, Zhe; Tong, Yigang

doi:10.1128/genomea.01754-15

Cited by 4 publications

(3 citation statements)

References 7 publications

(7 reference statements)

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“…Citrobacter spp. are opportunistic pathogens that can cause diarrhea, septicemia, meningitis, and urinary tract infections, especially in immunocompromised patients [27]. Together with our previous studies [10,16], we found 25 highly cytotoxic Citrobacter isolates out of 271 (9.2%), 15 were isolated from diarrheal patients (11.4% of diarrheal isolates), seven from foods (6.7% food isolates), two from healthy individuals (8.7%) and 1 from the environment (9.1%).…”

Section: Discussionmentioning

confidence: 99%

Lineage, Antimicrobial Resistance and Virulence of Citrobacter spp

et al. 2020

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Citrobacter spp. are opportunistic human pathogens which can cause nosocomial infections, sporadic infections and outbreaks. In order to determine the genetic diversity, in vitro virulence properties and antimicrobial resistance profiles of Citrobacter spp., 128 Citrobacter isolates obtained from human diarrheal patients, foods and environment were assessed by multilocus sequence typing (MLST), antimicrobial susceptibility testing and adhesion and cytotoxicity testing to HEp-2 cells. The 128 Citrobacter isolates were typed into 123 sequence types (STs) of which 101 were novel STs, and these STs were divided into five lineages. Lineages I and II contained C. freundii isolates; Lineage III contained all C. braakii isolates, while Lineage IV and V contained C. youngae isolates. Lineages II and V contained more adhesive and cytotoxic isolates than Lineages I, III, and IV. Fifty-one of the 128 isolates were found to be multidrug-resistant (MDR, ≥3) and mainly distributed in Lineages I, II, and III. The prevalence of quinolone resistance varied with Lineage III (C. braakii) having the highest proportion of resistant isolates (52.6%), followed by Lineage I (C. freundii) with 23.7%. Seven qnrB variants, including two new alleles (qnrB93 and qnrB94) were found with Lineage I being the main reservoir. In summary, highly cytotoxic MDR isolates from diarrheal patients may increase the risk of severe disease.

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Section: Discussionmentioning

confidence: 99%

Lineage, Antimicrobial Resistance and Virulence of Citrobacter spp

et al. 2020

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“…However, to our knowledge the information on genome sequences of C. freundii isolated from diseased fish was not available. Further, C. freundii has shown a strong ability to developing resistance to antibiotics used in human therapy and aquaculture ( 5 , 6 ). It is therefore imperative to advance our understanding of pathogenic, multidrug-resistant C. freundii strains associated with fish in order to identify antimicrobial resistance and virulence determinants.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequences of Citrobacter freundii Strains CF04 and A41 Isolated from Moribund, Septicemic Giant Gourami ( Osphronemus goramy) in Sri Lanka

et al. 2016

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“…Citrobacter freundii is a Gram negative, cocobacilli, lactose fermenter, facultative anaerobic member of the enterobacteriaceae family, which may be found in water, soil and in the gastrointestinal tracts of humans and animals. C. freundii causes urinary tract infections, wound infections, severe diarrhea, pneumonia, neonatal meningitis and brain abscesses in humans, especially in immunocompromised hosts [1]. C. freundii is considered as a major cause of nosocomial infections, characterized by inducible resistance mediated by the chromosomal AmpC β -lactamase [2].…”

Section: Introductionmentioning

confidence: 99%

Determination of some virulence factors of Citrobacter freundii isolated from Iraqi patients

Mahmood

Atyah

2021

eijs

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This study included the isolation and identification of Citrobacter freundii from 220 samples collected from inpatients and outpatients suffering from urinary tract infection (UTI) and identified at the laboratory of the General Samarra Hospital in Samarra City, Iraq. The study was conducted to investigate some of the virulence factors produced by C. freundii. The results showed that 67 isolates were belonging to the C. freundii, with a rate of 30.45%. Twenty eight samples were from inpatients (41.8%) and 39 samples were from outpatients. The bacterial identification was based on cultural and biochemical tests and confirmed by using VITEK2 compact system. Virulence factor results showed that all isolates were not blood hydrolyzing whereas they produced protease. Seven isolates (10.4%) produced biofilm, five from inpatients and two from outpatients, at rates of 17.8% and 5.1%, respectively. The results showed that 17 (25.4%) of the pathogenic isolates were β-lactamase producers, as determined by the iodometric method, twelve of them (17.9%) were from inpatients and 5 (7.5%) from outpatients. Four isolates of C. freundii produced Extended Spectrum Beta-lactamase (ESβL) enzymes, three from inpatients and one from outpatients, with ratios of 4.5% and 1.4%, respectively. Also, the via B gene, which is responsible for virulence factors, was investigated using PCR. The results showed that 12 isolates from inpatients and 4 isolates from outpatients were harboring this gene. The antimicrobial susceptibility testing by Kirby-Bauer’s method showed that all isolates that produced β-lactamase were resistant to antibiotics.

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Complete Genome Sequence of Multidrug-Resistant Citrobacter freundii Strain P10159, Isolated from Urine Samples from a Patient with Esophageal Carcinoma

Cited by 4 publications

References 7 publications

Lineage, Antimicrobial Resistance and Virulence of Citrobacter spp

Lineage, Antimicrobial Resistance and Virulence of Citrobacter spp

Draft Genome Sequences of Citrobacter freundii Strains CF04 and A41 Isolated from Moribund, Septicemic Giant Gourami ( Osphronemus goramy) in Sri Lanka

Determination of some virulence factors of Citrobacter freundii isolated from Iraqi patients

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