Draft Genome Sequences of
            <i>Citrobacter freundii</i>
            Strains CF04 and A41 Isolated from Moribund, Septicemic Giant Gourami (
            <i>Osphronemus goramy)</i>
            in Sri Lanka

Honein, Karim; Jagoda, S. S. S. De S.; Arulkanthan, A.; Ushio, Hideki; Asakawa, Shuichi

doi:10.1128/genomea.00820-16

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Strain A41 was found in GenBank as the closest relative genome of strain BR102 T and was included for further analysis. Strain A41 was isolated from moribund giant gourami (Osphronemus goramy) in Sri Lanka and it was previously identified as C. freundii although no information about the method used for identification at species level has been provided [10]. The average nucleotide identity (ANI) was calculated by JSpeciesWS [11] and digital DNA-DNA hybridization (dDDH) was performed using the Genome-to-Genome Distance Calculator under the recommended formula 2 [12].…”

Section: Genome Featuresmentioning

confidence: 99%

“…Gram staining was performed using a Gram-staining kit (bioMérieux). Growth at different NaCl concentrations (0, 3.0, 6.0, 9.0, 12.0 and 15.0 %, w/v) and temperatures (5,10,15,20,25,30,37, 42 and 50 °C) were examined by using TSB as the basal medium. Strain BR102 T was inoculated into TSB tubes with paraffin on top to confirm the anaerobic growth ability.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

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Citrobacter meridianamericanus sp. nov., isolated from a soil sample

Furlan

Ribeiro

Novais

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative strain, designated BR102T, isolated from a soil sample in Brazil was characterized by a polyphasic approach. Comparative 16S rRNA gene sequences indicated that strain BR102T belonged to the genus Citrobacter . The recN- and whole-genome-based phylogeny, and multilocus sequence analysis based on concatenated partial fusA, leuS, pyrG and rpoB sequences strongly supported a clade encompassing strain BR102T and a strain from public database that was distinct from currently recognized species of the genus Citrobacter . Average nucleotide identity and digital DNA–DNA hybridization values between strain BR102T and the closest relative Citrobacter freundii ATCC 8090T were 91.8 and 48.8 %, respectively. The ability to metabolize different compounds further discriminated strain BR102T from other closely related species of the genus Citrobacter . The novel variants bla CMY-179 and qnrB97, which encoded a CMY-2-like β-lactamase and a QnrB-type protein, respectively, were identified in strain BR102T. BR102T was resistant to ampicillin, amoxicillin/clavulanate and cefoxitin. The DNA G+C content of strain BR102T is 51.3 mol%. Based on these results, strain BR102T represents a novel species of the genus Citrobacter , for which the name Citrobacter meridianamericanus sp. nov. is proposed. The type strain is BR102T (=MUM 22.55T=IMI 507229T).

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Section: Genome Featuresmentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Citrobacter meridianamericanus sp. nov., isolated from a soil sample

Furlan

Ribeiro

Novais

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

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Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches

Liu

Han

et al. 2019

Ecotoxicology and Environmental Safety

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First report of Citrobacter freundii infection in Exotic Tropheus spp.

Dincturk,

Kumas,

Tanrikul

2024

Bulletin of the European Association of Fish Pathologists

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The ornamental fish industry has spread globally and it involves many different species of freshwater and marine fish. It is important to detect infectious diseases in aquarium fish due to their economic value and for the welfare of these animals. A case in which there was mass mortality of valuable exotic aquarium fish, Tropheus spp. was determined in July 2021, and the causative agent of infection was studied. The clinical and pathological symptoms of the disease were determined and the mortality rate recorded. After bacteriological examination, pure colonies were found to be present in tryptic soy agar (TSA) from bacterial isolations of the kidney, liver and spleen of the diseased fish. Gram stain, motility, oxidase and catalase activity of the isolated bacteria were determined and API 20E tests were used to provide the biochemical profile of the strain. Both juvenile and adult Tropheus spp. individuals were affected and 100% mortality was observed. Erratic swimming, inappetence, darkened skin, exophthalmus, abdominal distention, petechial hemorrhages on the skin, and hemorrhages on the fin bases and around the anus and mouth were found. Enteritis, gronulomas in the kidney, spleen, and pale liver were observed at necropsy. Gram-negative motile rods were identified as Citrobacter freundii by biochemical and 16s rRNA sequence analyses. To our knowledge, this is the first report of C. freundii infection in Tropheus spp., which is one of the valuable aquarium cichlid species.

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Draft Genome Sequences of Citrobacter freundii Strains CF04 and A41 Isolated from Moribund, Septicemic Giant Gourami ( Osphronemus goramy) in Sri Lanka

Cited by 3 publications

References 11 publications

Citrobacter meridianamericanus sp. nov., isolated from a soil sample

Citrobacter meridianamericanus sp. nov., isolated from a soil sample

Insight into the diversity of antibiotic resistance genes in the intestinal bacteria of shrimp Penaeus vannamei by culture-dependent and independent approaches

First report of Citrobacter freundii infection in Exotic Tropheus spp.

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