Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium

Lee, Kyung‐Hee; Bae, Dong Won; Kim, Sun Ho; Han, Hay Ju; Liu, Xiaomin; Park, Hyeong Cheol; Lim, Chae Oh; Lee, Sang Yeol; Chung, Woo Sik

doi:10.1016/j.jplph.2009.09.006

Cited by 157 publications

(101 citation statements)

References 46 publications

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“…Studies have found that beta-tubulin and alpha-1,4-glucan-protein synthase are involved in plant cell wall metabolism (SPOKEVICIUS et al, 2007;JIN et al, 2011). Lee et al (2010) also found that expression of alpha-1,4-glucan-protein synthase (A6) was significantly elevated. Membrane-bound ATPases provide energy and an H + gradient for the co-transport of copper, cobalt, lead, and Cd, with protons used for the detoxification of these metal ions in plants (CAO et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of saffron (Crocus sativus L.) grown under conditions of cadmium toxicity

Rao¹,

Lv²,

Yang³

2017

Biosci. J.

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Cd is a highly detrimental global environmental pollutant. Plants have evolved complex defense mechanisms as an adaptation to against Cd toxicity. In this study, a pot experiment was performed to evaluate the protein profile of saffron in response to Cd stress. Fifteen proteins were found to be up-regulated in the leaves of plants under Cd stress and were primarily related to metabolism, signal transduction, stress and defense response and energy. Eleven proteins were found to be down-regulated following Cd treatment, including ribulose bisphosphate carboxylase/oxygenase (Rubisco), ferredoxin-NADP reductase, a 70 kDa heat shock-related protein and three protein synthesis-associated proteins. The results provide valuable insights regarding the molecular mechanism of saffron in response to Cd toxicity and the possible utilization of genetic resources in developing Cd tolerant/low-accumulation saffron.

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of saffron (Crocus sativus L.) grown under conditions of cadmium toxicity

Rao¹,

Lv²,

Yang³

2017

Biosci. J.

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“…Both of two Grx genes have been reported in increased more than fivefold in rice roots subjected to juglone [9]. The up-regulated of GSTs has been shown in response to various heavy metals [20]. GSTs are known to catalyze the conjugation of heavy metals with reduced glutathione (GSH) and target them for ATPdependent transport into the vacuole.…”

Section: B Identify Asv-induced Genes Expression In Rice Rootsmentioning

confidence: 99%

Identification of Genes Related to Arsenic Detoxification in Rice Roots Using Microarray Analysis

Nguyen¹,

Huang²,

Huang³

2014

IJBBB

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Abstract-Arsenic(As) contamination in natural environment is a problem in many countries. Plants exposed to As show cytotoxic effects as inhibit cellular function, disrupt metabolism and reduce the yield. However, little is known about the molecular mechanisms of rice early expose to arsenate (AsV) at low concentration (10μM). Therefore, to better understand the molecular basis of rice cells, we performed a large-scale during analysis of the rice transcriptome within 24 hours challenged with AsV, one kind of common As inorganic species. In our microarray data, 614 transcripts were identified in response to AsV treatment. These transcripts related in plant defense mechanisms and the regulation of genes encoding to glutathione-S-transferase, oxidative stress, heat shock protein and enzymes in the ubiquitination pathway of protein degradation. Several important genes related to sulfate assimilation and GSH metabolism were induced for detoxification of As. Activities of peroxidase (POD) in rice roots increased after 12h and 24h treated to AsV, while the glutathione content at 12 h displayed decreasing trend but increasing trend after 24 h. Taken together, these data provide an overview of novel insights of AsV detoxification in rice and elicited for further investigation mechanism of arsenic detoxification in plants.

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“…Coomassie brilliant blue ve gümüş nitrat gibi boyama ajanları bitki proteomundaki ağır metal teşvikli değişimlerin analizinde sıklıkla kullanılmaktadır (Ahsan et al 2010;Lee et al 2010;Zhao et al 2011;Sharmin et al 2012 (Tonge et al 2001;Kieffer et al 2008;Evlard et al 2013;Printz et al 2013). 2-D DIGE tekniği, jeller arasında gözlenen varyasyonları azaltmakta ve üç farklı boyanın (Cy1, Cy2 ve Cy3) kullanımıyla tek bir jel üzerinde üç protein örneğinin karşılaştırılmasına olanak sağlamaktadır.…”

Section: Proteomik Teknikler Ve Stratejilerunclassified

“…Uzama faktörleri (EF) translasyonel uzamayı sağlayan bir protein setidir. Kloroplast translasyonel EF-Tu ve EF-P proteinlerinin miktarının Cd stresi altındaki Arabidopsis thaliana ve çeltik bitkilerinde arttığı belirtilmiştir (Ge et al 2009;Lee et al 2010;Semane et al 2010;Cai et al 2011). Kloroplastik EF-Tu'nun bitkilerde sıcaklık toleransına katkıda bulunduğu ve sıcaklığa hassas kloroplast proteinlerini termal çökelmeden ve inaktivasyondan koruyan bir moleküler şaperon olarak işlev gördüğü bildirilmiştir (Ristic et al 2007).…”

Section: Protein Metabolizması Ile İlişkili Proteinlerunclassified

“…(2007), çeltik bitkilerinde 27 glukanaz geninin ekspresyon seviyesini farklı gelişim evrelerinin yanı sıra biyotik ve abiyotik faktörlere cevap olarak karakterize etmiştir. Bununla birlikte, glukanaz ekspresyonunun ağır metaller tarafında da düzenlendiği proteomik yaklaşımla gösterilmiştir (Führs et al 2008;Kieffer et al 2008;Ahsan et al 2010;Lee et al 2010). Glukanazların ağır metal cevaplarındaki rolleri tam olarak açık olmamasına karşın, genel stres cevabı olarak düzenlendiği ileri sürülmüştür ).…”

Section: Patojenle İlişkili Proteinlerunclassified

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Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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ÖzetAğır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır. Heavy Metal Toxicity in Plants: Proteomics Approach Key wordsPlants; Heavy Metal; Toxicity; Proteomics; Mass Spectrometry AbstractHeavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed.

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Comparative proteomic analysis of the short-term responses of rice roots and leaves to cadmium

Cited by 157 publications

References 46 publications

Proteomic analysis of saffron (Crocus sativus L.) grown under conditions of cadmium toxicity

Proteomic analysis of saffron (Crocus sativus L.) grown under conditions of cadmium toxicity

Identification of Genes Related to Arsenic Detoxification in Rice Roots Using Microarray Analysis

Heavy Metal Toxicity in Plants: Proteomics Approach

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