Collection of Gingival Fluid for Quantitative Analysis

Sueda, Takeshi; Bang, J.; Cimasoni, G.

doi:10.1177/00220345690480011501

Cited by 64 publications

(31 citation statements)

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“…Using a capillary tube (5 μl), GCF samples were collected 28 by a single examiner (EMF) who was masked to the attribution of the sites to MPP or OFD. After the isolation and drying of the selected site with cotton rolls, disposable micropipettes ‖ were placed intrasulculary at the mesiofacial line angle of the selected site to a maximum depth of 2 mm below the gingival margin.…”

Section: Methodsmentioning

confidence: 99%

Platelet‐Derived Growth Factor‐BB Release Profile in Gingival Crevicular Fluid After Use of Marginal Periosteal Pedicle Graft as an Autogenous Guided Tissue Membrane to Treat Localized Intrabony Defects

Gamal¹,

El-Shal²,

El-Aasara³

et al. 2011

Journal of Periodontology

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Section: Methodsmentioning

confidence: 99%

Platelet‐Derived Growth Factor‐BB Release Profile in Gingival Crevicular Fluid After Use of Marginal Periosteal Pedicle Graft as an Autogenous Guided Tissue Membrane to Treat Localized Intrabony Defects

Gamal¹,

El-Shal²,

El-Aasara³

et al. 2011

Journal of Periodontology

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“…[10] A standard volume of 1.2 μl of GCF was collected in a glass capillary tube with an internal diameter of 1.0 mm, as described by Sueda et al . [11] The collected fluid was immediately transferred into a microcentrifugation plastic tube containing 60 μl of calibration diluent RD6X.…”

Section: Methodsmentioning

confidence: 99%

A comparative evaluation of hepatocyte growth factor levels in gingival crevicular fluid and saliva and its correlation with clinical parameters in patients with and without chronic periodontitis: A clinico-biochemical study

Rudrakshi

Srinivas

Mehta

2011

J Indian Soc Periodontol

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Objectives:The purpose of the present study was to evaluate and compare the relationship between the levels of hepatocyte growth factor (HGF) in gingival crevicular fluid (GCF) and saliva, both clinically and biochemically, in patients with and without chronic periodontitis.Materials and Methods:For this study, 36 patients (12 male, 24 female) were selected in the age range of 18-60 years (mean, 32.7±11.1 years). The subjects were assigned to three groups, which included Group I (control), Group II (chronic periodontitis with probing depth PD <5 mm), and Group III (chronic periodontitis with PD ≥5 mm). Clinical parameters included plaque index, gingival index, sulcus bleeding index, PD, and clinical attachment level. The GCF samples were taken by using the capillary tubes whereas saliva was collected by the suction method. The levels of HGF in GCF and in saliva were estimated using an enzyme linked immunosorbant assay reader.Results:There was a significant correlation in the levels of HGF in GCF and in saliva of patients with and without chronic periodontitis. The results also indicated that the HGF levels in GCF and saliva correlated well with the clinical parameters and with the severity of the periodontal disease.Conclusion:Both GCF and saliva can be used to estimate the levels of HGF and thus may be regarded as a novel marker for periodontal disease activity.

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“…Thus, the use of microcapillary pipettes for GCF collection is not a problem for these patients. Also, it has the advantage as it is not plagued by the nonspecific attachment of the analyte to the filter paper (20). …”

Section: Discussionmentioning

confidence: 99%

Salivary and gingival crevicular fluid histatin in periodontal health and disease

Bhadbhade¹,

Acharya

Thakur

2013

J Clin Exp Dent

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Objectives: Histatin, with its anti bacterial, anti protease, and wound closure stimulating property might influence the pathogenesis of periodontal disease. This study assessed the presence of histatin in gingival crevicular fluid (GCF); the levels of salivary and GCF histatin in periodontal disease. Material and methods: It was a cross sectional study that included systemically healthy forty five subjects (22 males and 23 females) between the age group of 20 to 45 years. Based on Gingival Index (Loe and Silness ,1963) and Russell’s Periodontal Index they were grouped as 15 healthy (Group 1), 15 gingivitis (Group 2), and 15 periodontitis (Group 3) subjects. Whole pooled unstimulated saliva was collected by asking the patient to spit in a sterile container and GCF samples were collected using a micropipette from all the subjects. Histatin levels were assessed using Enzyme Linked Immunosorbent Assay (ELISA). The intergroup comparison was done by ANOVA and Mann Whitney U Test was done for pair wise comparison. Results: The results of this study show that histatin is present in saliva and gingival crevicular fluid. When the salivary histatin levels were compared it was found that the levels of histatin increase from health to periodontitis but the levels of histatin in the gingival crevicular fluid and saliva had no correlation with severity of periodontal disease as there was no statistically significant difference between the three groups. Conclusions: It can be concluded that histatin cannot be used as a potential marker of periodontal disease. Key words:Periodontal disease, histatin, gingival crevicular fluid, saliva, gingival index, periodontal index, enzyme linked immunosorbent assay.

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Collection of Gingival Fluid for Quantitative Analysis

Cited by 64 publications

References 0 publications

Platelet‐Derived Growth Factor‐BB Release Profile in Gingival Crevicular Fluid After Use of Marginal Periosteal Pedicle Graft as an Autogenous Guided Tissue Membrane to Treat Localized Intrabony Defects

Platelet‐Derived Growth Factor‐BB Release Profile in Gingival Crevicular Fluid After Use of Marginal Periosteal Pedicle Graft as an Autogenous Guided Tissue Membrane to Treat Localized Intrabony Defects

A comparative evaluation of hepatocyte growth factor levels in gingival crevicular fluid and saliva and its correlation with clinical parameters in patients with and without chronic periodontitis: A clinico-biochemical study

Salivary and gingival crevicular fluid histatin in periodontal health and disease

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