A comparative evaluation of hepatocyte growth factor levels in gingival crevicular fluid and saliva and its correlation with clinical parameters in patients with and without chronic periodontitis: A clinico-biochemical study

Abstract

Objectives:The purpose of the present study was to evaluate and compare the relationship between the levels of hepatocyte growth factor (HGF) in gingival crevicular fluid (GCF) and saliva, both clinically and biochemically, in patients with and without chronic periodontitis.Materials and Methods:For this study, 36 patients (12 male, 24 female) were selected in the age range of 18-60 years (mean, 32.7±11.1 years). The subjects were assigned to three groups, which included Group I (control), Group II (chronic periodontitis with probing depth PD <5 mm), and Group III (chronic periodontitis with PD ≥5 mm). Clinical parameters included plaque index, gingival index, sulcus bleeding index, PD, and clinical attachment level. The GCF samples were taken by using the capillary tubes whereas saliva was collected by the suction method. The levels of HGF in GCF and in saliva were estimated using an enzyme linked immunosorbant assay reader.Results:There was a significant correlation in the levels of HGF in GCF and in saliva of patients with and without chronic periodontitis. The results also indicated that the HGF levels in GCF and saliva correlated well with the clinical parameters and with the severity of the periodontal disease.Conclusion:Both GCF and saliva can be used to estimate the levels of HGF and thus may be regarded as a novel marker for periodontal disease activity.

Periodontal Treatment Downregulates Protease-Activated Receptor 2 in Human Gingival Crevicular Fluid Cells

“…In addition, in the present study we demonstrated that systemically healthy periodontitis patients have elevated levels of HGF in the crevicular fluid, which is in agreement with other studies from the literature (43)(44)(45). We also observed decreased HGF concentration after periodontal treatment.…”

Comparative evaluation of salivary hepatocyte growth factor in smokers and non‐smokers with chronic periodontitis

“…Thus, as HGF concentration increased proportionally with the progression of PD, and HGF concentrations showed a positive correlation with clinical parameters, we can conclude that HGF plays a key role in PD progression. The findings of our study are in accordance with other investigations, which obtained similar results . The results of the present study showed that HGF levels increased progressively in saliva from health to periodontitis and decreased after periodontal therapy in periodontitis‐affected individuals.…”

“…Intergroup comparisons between the test group and control groups for clinical parameters (i.e PI, SBI, PPD, and CAL) showed significantly higher values in the test groups compared to the control group (Table ). Comparison between the two test groups showed that all the clinical parameters, except for SBI, were significantly higher in group 3 compared to group 2, which was in accordance to other studies that showed similar results . The mean SBI score was significantly higher in group 2 compared to groups 1 and 3, and comparison between the two test groups showed higher values in group 2 compared to group 3.…”

“…GCF and saliva are important biologic samples that determine disease activity. Salivary HGF levels have been positively correlated with clinical parameters, and it has been suggested that salivary HGF could be a novel marker for periodontal diagnosis . As it is uncomplicated and non‐invasive, saliva sampling was used in the present study to estimate HGF activity.…”

Evolution of Periodontal Disease: Immune Response and RANK/RANKL/OPG System

“…Several studies have investigated the role of NO in the progression of periodontal diseases in humans, as well as in ligature-induced periodontitis in rats, demonstrating its increasing participation during the progression of periodontal disease. The nitric oxide concentration can therefore be used as a marker of inflammation, indicating the status of the pathology (18,19). Elevated HGF levels have been found in patients suffering chronic diseases including periodontitis (9).…”