Co60 Vit. B12 Binding Capacity of Normal Human Cerebrospinal Fluid.

Abstract

CSF CoGO BI2 BINDING CAPACITY 60 7 animal. In our experiments doses several times the lethal dose elicited no responses which suggested release of significant amounts of histamine, Summary. 1) In anesthetized rabbits, lethal doses of compound 48/80 produced marked depression of respiration with ensuing blood pressure changes that could be altered by administering artificial respiration. Blood pressure and electrocardiographic records in animals artificially respired soon after receiving doses several times the lethal dose revealed no evidence of adverse effects on the vascular system or on cardiac rate and rhythm. 2 ) Under similar conditions, however, a lethal dose of histamine produced a prompt and profound fall in blood pressure, marked changes in cardiac rate and rhythm and death despite artificial respiration. The effect appears to be primarily on the cardiovascular system. Respiratory effects are prob-aldy secontlary to this main action. 3 ) The lethal action of 48/80 in rabbits is apparently not mediated by release of endogenous histamine since large doses do not elicit histaminelike responses.

Vitamin B12 levels in the serum and cerebrospinal fluid in multiple sclerosis

“…Both these differences suggest that vitamin B12 binding in serum may differ from that in cerebrospinal fluid. Meyer, Bertcher, and Mulzac (1959) found in dialysis experiments with cerebrospinal fluid and radioactive vitamin B,2 that the ratio of radioactive vitamin binding capacity to protein concentration was higher for cerebrospinal fluid than for serum. They concluded that in cerebrospinal fluid vitamin B12 was not bound to protein or that this protein was different from that in serum.…”

Observations on vitamin B12 in serum and cerebrospinal fluid in multiple sclerosis

“…No explanation for this can be suggested without further investigation, and it is unlikely to be explained until the factors governing the concentration of B12 in cerebrospinal fluid are better understood. These probably include not only the concentration of B12 in serum, but the permeability of the bloodbrain barrier, the B12-binding capacity of the cerebrospinal fluid (Meyer, Bertcher, and Mulzac, 1959;Clausen and Munkner, 1962;Worm-Petersen, 1962) and perhaps utilization by the brain. Our finding of normal concentrations of total serum B12 in multiple sclerosis confirms the reports of Grann andGlass (1961) andWorm-Petersen (1962).…”

Vitamin B12 levels of cerebrospinal fluid in patients with a variety of neurological disorders.

“…The binding capacity of CSF protein has been examined by MEYER et al . (18) and WORM-PETERSEN (19). According to them , the B12 binding capacity of CSF protein is higher than that of serum (18) and the nature of the protein is different from that in serum (19).…”