Observations on vitamin B12 in serum and cerebrospinal fluid in multiple sclerosis

Basil, W.; Brown, J. E.; Matthews, D. M.

doi:10.1136/jcp.18.3.317

Cited by 22 publications

(16 citation statements)

References 14 publications

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“…B12 levels in the CSF is only 5-10% of the level found in serum (1) . Mean CSF B12 levels on 27 control subjects was 21 .2pg/ml, well in agreement with pre vious reports (8,11,12,19). Although significant correlation was demonstrated between serum and CSF B12 levels in the reports of WORM-PETERSEN (8) and SIMP SON (11) and BASIL et al (12), we avoided observing the correlation between serum and CSF B12 levels in the present study because the microbiological assay is thought to be inadequate to have a good reproducibility at such a low concentration of B 12.…”

Section: Methodssupporting

confidence: 90%

“…There is very little infor mation concerning the transport of B12 from serum to CSF or vice versa . BASIL et al (12) reported high CSF B12 (60-120pg/ml) in B12-treated multiple sclerosis .…”

Section: Resultsmentioning

confidence: 97%

“…Nevertheless, UKYO (6) reported that intrathecal B12 administration produced some improvement in neurological manifestations in SMON. Al though many papers have reported B12 levels in cerebrospinal fluid (CSF) (7)(8)(9)(10)(11)(12)(13)(14) , little is known about the transport of B12 from the blood to the CSF (15) and vice versa (6,16). The purposes of this paper are to report CSF B12 levels in various neurological diseases especially in SMON and to see whether blood B12 is easily transported to and from the CSF.…”

Section: Discussionmentioning

confidence: 99%

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Vitamin B12 levels of cerebrospinal fluid in patients with a variety of neurological disorders.

Taguchi

Sanada

Hara

et al. 1977

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe vitamin B12 levels of cerebrospinal fluid were assayed microbiologically (Lactobacillus leichmannii method) using samples from 44 patients with various neurological disorders, 4 patients with megalo blastic anemia and 34 controls. Twenty-seven controls that did not receive vitamin B12 showed a mean cerebrospinal fluid vitamin B12 level of 21.5pg/ml (range: 0-60). No decrease in cerebrospinal fluid vitamin B12 level was seen in patients with subacute myelo-optico-neuropathy (SMON). High levels of cerebrospinal fluid vitamin B12 were observed only in the patients receiving long term administration of the vitamin. Intrathecal administration of vitamin B12 caused only a slight increase in serum vitamin B12 level after four hours. The existence of a blood brain barrier for vitamin B12 was suggested.

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Section: Methodssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamin B12 levels of cerebrospinal fluid in patients with a variety of neurological disorders.

Taguchi

Sanada

Hara

et al. 1977

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…Nevertheless, cobalamin-deficient subjects also had a lower CSF level of the vitamin in comparison to levels measured in non-deficient subjects (67). The correlation between blood and CSF cobalamin contents was not confirmed in some studies (68,69). However, these studies utilized old methods and cobalamin concentrations were at the lowest detectable limit which may imply large impressions.…”

Section: Brain and Csf Cobalaminmentioning

confidence: 92%

Biomarkers of folate and vitamin B12 status in cerebrospinal fluid

Herrmann¹,

Obeid²

2007

Clinical Chemical Laboratory Medicine

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Folate and vitamin B(12) are essential cofactors for the methionine/homocysteine cycle in the brain. These vitamins mediate the remethylation of homocysteine (Hcy), which affects the production of the universal methyl donor, S-adenosylmethionine (SAM), in the brain among other organs. Additionally, increased plasma concentrations of total Hcy (tHcy) are associated with cerebrovascular disease and can compromise the blood-brain barrier. tHcy concentrations in the brain and cerebrospinal fluid become increased in several psychiatric and neurological disorders. Disturbances in the transmethylation pathway indicated by abnormal SAM, S-adenosylhomocysteine or their ratio have been reported in many neurodegenerative diseases, such as dementia, depression or Parkinson's disease. Cobalamin is essential for neuronal generation and its deficiency can cause degeneration of the nervous system. Available data emphasize that deficiency of folate and vitamin B(12) can lead to elevated concentrations of tHcy and disturbed methylation potential in the brain. Therefore, acquired or inherited disorders in these metabolic pathways are associated with brain abnormalities and severe neurological symptoms that are mostly irreversible, even after providing the missing cofactors. This review discusses the relationship between brain and blood levels of key vitamins and metabolites related to one carbon metabolism.

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“…5 In other studies, the frequency of severe B 12 deficiency is reported to be low. 6,7 Two important enzyme reactions are dependent on adequate vitamin B 12 concentrations in the cell. Malfunction of these enzymes due to vitamin B 12 deficiency leads to high cellular concentrations of methylmalonic acid (MMA) and homocysteine (tHcy), followed by increased concentrations in other biological fluids.…”

Section: Introductionmentioning

confidence: 99%

Increased plasma homocysteine levels without signs of vitamin B12 deficiency in patients with multiple sclerosis assessed by blood and cerebrospinal fluid homocysteine and methylmalonic acid

Vrethem¹,

Mattsson

Hebelka

et al. 2003

Mult Scler

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The relevance of the increased mean value of plasma tHcy thus seems uncertain and does not indicate functional vitamin B12 deficiency. We can not, however, exclude the possibility of a genetically induced dysfunction of the homocysteine metabolism relevant for the development of neuroinflammation/degeneration. Our findings indicate that, regardless of a significant increase in plasma tHcy in MS patients, the MS disease is not generally associated with vitamin B12 deficiency since we did not find any other factors indicating vitamin B12 deficiency. Analysis of CSF MMA and CSF tHcy, which probably reflects the brain vitamin B12 status better than serum, are not warranted in MS. We conclude that B12 deficiency, in general, is not associated with MS.

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Observations on vitamin B12 in serum and cerebrospinal fluid in multiple sclerosis

Cited by 22 publications

References 14 publications

Vitamin B12 levels of cerebrospinal fluid in patients with a variety of neurological disorders.

Vitamin B12 levels of cerebrospinal fluid in patients with a variety of neurological disorders.

Biomarkers of folate and vitamin B12 status in cerebrospinal fluid

Increased plasma homocysteine levels without signs of vitamin B12 deficiency in patients with multiple sclerosis assessed by blood and cerebrospinal fluid homocysteine and methylmalonic acid

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