Chapter III. Gas-Liquid Chromatographic Chemotaxonomy

Drucker, D.B.

doi:10.1016/s0580-9517(09)70040-6

Cited by 30 publications

(15 citation statements)

References 65 publications

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“…except that the relative proportions of 3OH-C 14:O were higher in the current study (15 to 30 per cent). In the literature, cellular fatty acids have been reported to be influenced by changes in cultural conditions (Drucker 1976). However, in the current study, varying the cultural media, the incubation atmosphere or the age at which the culture was harvested had no significant effect on the cellular fatty acids of the reference strains.…”

Section: Dlscussloncontrasting

confidence: 71%

Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Neill

O'Brien

McMurray

et al. 1984

Equine Veterinary Journal

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Summary Cellular fatty acid compositions of contagious equine metritis isolates and three reference Haemophilus equigenitalis cultures were determined by gas chromatography. The chromatographic data were standardised and normalised fatty acid methyl ester (FAME) profiles were produced. The profiles were compared visually and similarity indices were determined using a computer peak matching method. There was little difference between the profiles of the three reference strains, each strain being characterised by three major fatty acids; C 18:1, C 16:0 and 30H‐C 14:0. Variations in cultural conditions had no significant effect on the FAME profiles. The identification of laboratory isolates using the technique was in agreement with the presumptive identification based on the currently recommended tests and an improvement on the confirmatory serological identification. The FAME profiles provided confirmation of identity where it was not possible to use the presently recommended serological procedures. The authors recommend the gas chromatography technique for use in the diagnostic laboratory as an adjunct to the presently accepted identification methods.

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Section: Dlscussloncontrasting

confidence: 71%

Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Neill

O'Brien

McMurray

et al. 1984

Equine Veterinary Journal

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“…control. The presence of certain fatty acids in bacteria has been shown to correlate with taxonomic conventions (Drucker, 1976;Lechevalier, 1977). Hence the patterns of fatty acids, once identified, have resulted in the production of 'fingerprints' of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the fatty acid profiles of Borrelia, Serpulina and Leptospira species

Livesley¹,

Thompson²,

Bailey³

et al. 1993

Journal of General Microbiology

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Fatty acid methyl ester (FAME) derivatives were examined as a means of characterizing Borrelia burgdorferi isolates and distinguishing them from other spirochaetes. Analysis was performed using a gas liquid chromatography column in conjunction with Microbial Identification System (MIS) software. Reproducible FAME profiles were produced which distinguished Borrelia species, Serpulina hyodysenteriae and Leptospira ictevohaemorrhagiae. Furthermore, the FAME profiles of four recognized Borrelia species (including two American isolates of Borrelia burgdorferi, B31 and JDI) were distinct from one another and from the BSK I1 medium in which they were grown. The results confirm previous reports that FAME profiles of bacteria represent a diagnostic phenotypic property and suggest that they may have applications in the chemotaxonomic classification of Borrelia species.

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“…Similarities were calculated with the Bravais-Pearson coefficient of linear correlation (Drucker, 1976). The square roots of the data were used to reduce the influence of the larger peaks on the analysis.…”

Section: Methodsmentioning

confidence: 99%

Analysis of bacterial phospholipid markers and plant monosaccharides during forage degradation by Ruminococcus flavefaciens and Fibrobacter succinogenes in co-culture

Saluzzi¹,

Smith²,

Stewart³

1993

Journal of General Microbiology

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~~ ~~ ~ ~~~Marker components of the phospholipids of Ruminococcus flavefaciens and Fibrobacter succinogenes were identified for studies on the degradation of forage by these bacteria growing in mixed culture. The principal fatty acid methyl esters and dimethyl acetals detected varied between strains and were influenced by the addition of a mixture of higher volatile fatty acids and vitamins to the medium, but these effects were small compared to the differences between the species. When two strains of R. flavefaciens were grown on a mixture of clover and ryegrass, and on barley straw in the presence or absence of two strains of F. succinugenes, the solubilization of plant material tended to be lowered by the presence of F. succinugenes. R.flavefaciens was the predominant bacterium among colonies recovered from roll tubes, and the phospholipids were primarily those of R. flavefaciens. Analysis of the culture supernatant liquids showed that F. succinogenes produced greater amounts of free and bound xylose from both clover and straw than did R. flauefaciens. With both forages, cultures containing the two species produced more soluble free arabinose, and less soluble-bound arabinose, than either species grown alone.

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Chapter III. Gas-Liquid Chromatographic Chemotaxonomy

Cited by 30 publications

References 65 publications

Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Comparison of the fatty acid profiles of Borrelia, Serpulina and Leptospira species

Analysis of bacterial phospholipid markers and plant monosaccharides during forage degradation by Ruminococcus flavefaciens and Fibrobacter succinogenes in co-culture

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