Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Neill, S.D.; O'Brien, JJ; McMurray, C.H.; Blanchflower, W. J.

doi:10.1111/j.2042-3306.1984.tb01966.x

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…18 : 0), the profiles of T. equigenitalis and the donkey isolates were similar. The characteristics shown are in agreement with those reported for T. equigenitalis (Neill et al, 1984 ;Sugimoto et al, 1982 ;Vandamme et al, 1998).…”

Section: Cellular Fatty Acid Analysissupporting

confidence: 91%

Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus).

Jang¹,

Donahue²,

Arata³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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Three bacterial isolates that were phenotypically indistinguishable from Taylorella equigenitalis were obtained from the urethral fossae of three male donkeys (Equus asinus), one located in the state of California and the other two in the state of Kentucky, USA. Based on results of pulsed-field gel electrophoresis, the isolate from California differed from the two Kentucky isolates, which were the same. Mares bred artificially (California) or naturally (Kentucky) did not show signs of disease, even though infection with the organism was established in those bred naturally. Mares and, uncharacteristically, all three jacks produced antibodies that reacted in the complement fixation test utilized to identify mares recently infected with T. equigenitalis. Sequence analysis of DNA encoding the 16S rRNA revealed that the gene sequences of these isolates were virtually identical to each other (>99.8% similarity), but different (97.6% similarity) from those of several confirmed isolates of T. equigenitalis. The 16S rDNA sequences of the latter were 100% identical. DNA-DNA hybridization studies revealed a mean hybridization level of 89% between the donkey isolate from California and the donkey isolate from Kentucky. On the other hand, the mean DNA-DNA hybridization level from the donkey isolates with DNA from a strain of T. equigenitalis was 23%. The DNA G+C composition was 37.8 mol% for the two donkey isolates, as well as the strain of T. equigenitalis used in the hybridization studies. These data support our opinion that micro-organisms isolated from the male donkeys are different from T. equigenitalis and it is proposed that they be considered a new species within the genus Taylorella and named Taylorella asinigenitalis sp. nov. The type strain is strain UCD-1T (= ATCC 700933T = LMG 19572T).

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Section: Cellular Fatty Acid Analysissupporting

confidence: 91%

Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus).

Jang¹,

Donahue²,

Arata³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…After 1 h, 400 ,ul of buffer was removed and 20 U of restriction endonuclease (GIBCO/BRL or New England BioLabs, Inc.) was added. Restriction digestion was performed for 6 to 12 h. 8.3]-44.5 mM boric acid-1.25 mM disodium EDTA) at 90 V. The FIGE program consisted of 6 to 12 identical cycles of 4 h. An exponentially increasing forward switch time (0.05 to 55 s) was used; 50% of the switch time was reached at 40% of the cycle time. The reverse time phase and the pause time were 33 and 2%, respectively, of the forward time phase.…”

Section: Methodsmentioning

confidence: 99%

Epidemiologic study of Taylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments

1990

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Contagious equine metritis (CEM), a sexually transmitted bacterial disease, was first described in thoroughbred horses. It also occurs in nonthoroughbred horses, in which it produces isolated, apparently unrelated outbreaks. Thirty-two strains of Taylorella equigenitalis, the causative agent of CEM, from all over the world were characterized by field inversion gel electrophoresis of fragments of genomic DNA obtained by digestion with low-cleavage-frequency restriction enzymes. This resulted in a division into five clearly distinct groups. Strains from thoroughbred horses from all continents belonged to one group. Strains from nonthoroughbred horses from various countries were different from strains from thoroughbred horses; four groups could be determined. Two groups contained both streptomycin-resistant and streptomycin-susceptible strains. The data indicate that CEM in nonthoroughbreds did not originate from the thoroughbred population; also, the reverse was not demonstrated. Thus, extensive international transportation directives regarding the testing of nonthoroughbred horses for CEM may need reconsideration.

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“…Cellular fatty acid composition of T. equigenitalis was determined by gas chromatography. Fatty acid methyl ester (FAME) profiles have shown that T. equigenitalis contains 3 major fatty acids including C 18: 1, C 16:0, and 30H-C 14:0 (22).…”

Section: Introductionmentioning

confidence: 99%

The development of a new selective medium for isolation of Taylorella sp.

Carew¹

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The objective of this study was to develop an improved selective medium for the isolation of Tay/ore/la sp .. It is important the United States remains free of contagious equine metritis, because large sums of money could be lost in the Thoroughbred industry if this organism is not easily detected. The currently used selective medium, Modified Timoney Shin (MTS), is too restrictive for growing some strains of Tay/ore/la sp. A new selective media that is more restrictive to growth of contaminants would ensure that laboratory personnel are able to isolate Tay/ore/la sp. more readily from diagnostic samples. A total of eighty-seven antimicrobials were evaluated using a disk diffusion test, and ten antimicrobials with the smallest mean zones of inhibition for Tay/ore/la sp. over three replications were chosen for inclusion in this study. The zones of inhibition of Tay/ore/la equigenitalis strains were significantly larger than the zones of inhibition of Tay/ore/la asinigenitalis strains for some of the antimicrobials. Combinations of antimicrobials were evaluated, and final selections were based on the maximum number of contaminants inhibited and on the ability to support the growth of T. equigenita/is and T. asinigenita/is. The combination of 320 µg/ml paminosalicyclic acid, 5 µg/ml clindamycin, and 5 µg/ml amphotericin B supported the growth of Tay/ore/la sp., but was more inhibitory to the ten most frequent contaminants than was MTS. Ten individual contaminants and sixteen combinations of a single dilution of contaminants with Tay/ore/la sp. included in lower dilutions were plated onto the new medium and MTS. Fourteen contaminants were more inhibited on the new medium than on MTS, eleven had comparable growth on both, and there was one that grew better on the new medium. Twenty-two swabs from caudal reproductive tracts of horses were evaluated.Sixteen had less growth on the new medium than on MTS, four had comparable growth, and V two had more growth. A side by side comparison of the new media with MTS will be necessary to evaluate its potential to replace MTS in diagnostic testing.antifungal agent and two antibiotics, is too restrictive for growth of some strains of Tay/ore/la sp.Under the present system, the United States National Veterinary Services Laboratories (NVSL) approves state and university diagnostic laboratories for conducting CEM testing on imported horses. Swabs from mares (cervix, clitoral fossa, and clitoral sinus) and stallions (urethra, urethral fossa, and penile sheath) are submitted to diagnostic laboratories for culture. The horses are held in quarantine stations until testing is completed. If Tay/ore/la sp. are detected, horses must be treated under a specific regimen and retested before they are released, or they must be returned to the country of origin. If carrier horses were missed due to inadequate bacteriologic culture, very expensive epidemiologic tracing would be required to assure that all potentially exposed horses were tested . Development of a new selective medium which would inh...

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Contagious equine metritis — Use of gas liquid chromatography in identifying the causal agent

Cited by 4 publications

References 12 publications

Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus).

Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus).

Epidemiologic study of Taylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments

The development of a new selective medium for isolation of Taylorella sp.

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