Analysis of bacterial phospholipid markers and plant monosaccharides during forage degradation by Ruminococcus flavefaciens and Fibrobacter succinogenes in co-culture

Saluzzi, L.; Smith, Alistair; Stewart, C. S.

doi:10.1099/00221287-139-11-2865

Cited by 24 publications

(30 citation statements)

References 35 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation is consistent with the reported by other researchers (Collings and Yokoyama, 1980; Saluzzi et al, 1993). Studies have also shown that strains of R. flavefaciens have the ability to degrade noncellulosic polysaccharide components of plant cell walls because they produce a variety of other polysaccharidases including xylanase and pectinase (Flint et al, 1989; Erfle and Teather, 1991).…”

Section: Discussionsupporting

confidence: 94%

Effect of Exogenous Fibrolytic Enzyme Application on the Microbial Attachment and Digestion of Barley Straw In vitro

Wang

Ramírez-Bribiesca

Yanke

et al. 2011

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

The effects of exogenous fibrolytic enzymes (EFE; a mixture of two preparations from Trichoderma spp., with predominant xylanase and β-glucanase activities, respectively) on colonization and digestion of ground barley straw and alfalfa hay by Fibrobacter succinogenes S85 and Ruminococcus flavefaciens FD1 were studied in vitro. The two levels (28 and 280 μg/ml) of EFE tested and both bacteria were effective at digesting NDF of hay and straw. With both substrates, more NDF hydrolysis (p<0.01) was achieved with EFE alone at 280 than at 28 μg/ml. A synergistic effect (p<0.01) of F. succinogenes S85 and EFE on straw digestion was observed at 28 but not 280 μg/ml of EFE. Strain R. flavefaciens FD1 digested more (p<0.01) hay and straw with higher EFE than with lower or no EFE, but the effect was additive rather than synergistic. Included in the incubation medium, EFE showed potential to improve fibre digestion by cellulolytic ruminal bacteria. In a second batch culture experiment using mixed rumen microbes, DM disappearance (DMD), gas production and incorporation of 15N into particle-associated microbial N (15N-PAMN) were higher (p<0.001) with ammoniated (5% w/w; AS) than with native (S) ground barley straw. Application of EFE to the straws increased (p<0.001) DMD and gas production at 4 and 12 h, but not at 48 h of the incubation. EFE applied onto S increased (p<0.01) 15N-PAMN at 4 h only, but EFE on AS increased (p<0.001) 15N-PAMN at all time points. Prehydrolysis increased (p<0.01) DMD from both S and AS at 4 and 12 h, but reduced (p<0.01) 15N-PAMN in the early stage (4 h) of the incubation, as compared to non-prehydrolyzed samples. Application of EFE to barley straw increased rumen bacterial colonization of the substrate, but excessive hydrolytic action of EFE prior to incubation decreased it.

show abstract

Section: Discussionsupporting

confidence: 94%

Effect of Exogenous Fibrolytic Enzyme Application on the Microbial Attachment and Digestion of Barley Straw In vitro

Wang

Ramírez-Bribiesca

Yanke

et al. 2011

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

show abstract

“…Fatty acid analysis of the product recoverd from the phenol phase of OM extract showed the presence of the same fatty acids. No 3-hydroxy acids were detected in either fraction, which agrees with previously published results for fatty acid analysis of F. succinogenes [16].…”

Section: Component Analysis General Characterization and Chemical Desupporting

confidence: 92%

Structural analysis of the carbohydrate components of the outer membrane of the lipopolysaccharide‐lacking cellulolytic ruminal bacterium Fibrobacter succinogenes S85

Vinogradov

Egbosimba

Perry

et al. 2001

European Journal of Biochemistry

View full text Add to dashboard Cite

The polysaccharides from the outer membrane of the Gramnegative ruminal bacterium Fibrobacter succinogenes were isolated by phenol/water extraction and separated by sizeexclusion chromatography in the presence of deoxycholate detergent into a lower-molecular-mass fraction designated glycolipid' and a high-molecular-mass`capsular polysaccharide' fraction. Both fractions lacked typical lipopolysaccharide components including 2-keto-3-deoxyoctulosonic acid and 3-hydroxy fatty acids. Carbohydrate components of these fractions were represented by two polysaccharides and one oligosaccharide (possibly glycolipid) with the following structures:where HEAEP is N-(2-hydroxyethyl)-2-aminoethylphosphonic acid, found for the first time in natural compounds. The polysaccharides contained pentadecanoic acid and anteisopentadecanoic acid, possibly present as the acyl components. All constituent monosaccharides except l-rhamnose had a d-configuration.In addition to having a structural role in the outer membrane, these polysaccharides may provide protection for this lipopolysaccharide-less bacterium in the highly competitive ruminal environment, as phosphonic acids covalently linked to membrane polymers have in the past been attributed the function of stabilizing membranes in the presence of phosphatases and lipases.Keywords: Fibrobacter succinogenes; glycolipid; NMR; polysaccharide; structure.The outer membrane (OM) of Gram-negative bacteria serves as a permeability barrier to a wide variety of compounds [1]. Types of compounds excluded are hydrophobic antibiotics (e.g. macrolides, novobiocins, rifamycins, actinomycin D, fusidic acid), detergents (e.g. bile salts, SDS, Triton X-100), and hydrophobic dyes (e.g. eosin, methylene blue, brilliant green acridine dyes).Lipopolysaccharide molecules are located on the outer leaflet of the OM and provide the key barrier function, low fluidity, and tight architecture of the OM [1,2]. In addition to barrier functions, lipopolysaccharide has a role in the adhesion of bacteria to various mammalian cell types [3]. Gram-negative ruminal bacteria live in the highly competitive intestinal environment containing varying concentrations of fatty acids, bacteriocins [4] and other types of inhibitory molecules [5,6].Fibrobacter succinogenes S85 is a highly cellulolytic Gram-negative ruminal bacterium. It has been shown to adhere to substrates by means of extracellular polymeric material (glycocalyx) [7]. The nature and composition of this coat has never been investigated. However, being a Gram-negative organism, it may be composed of cell surface capsular polysaccharide (CPS), lipopolysaccharide, or a combination of the two. Information on the structure and biology of the surface polysaccharides from this bacterium will contribute to our understanding of the role of these exopolysaccharides.In this study, we isolated and purified the polysaccharides associated with the OM of F. succinogenes S85 and characterized their structures. Eur. J. Biochem. 268, 3566±3576 (2001) ); Kdo, 3-deoxy-d-manno-oct-2-u...

show abstract

“…The xylanases of F. succinogenes have higher activity than those of ruminococci (Saluzzi et al 1993), and are thought to act to expose cellulose in hemicellulose and/or cellulose complexes. When F. succinogenes S85 is incubated with wheat straw, acetylxylan esterase and arabinofuranosidase activities are also detected at high levels (Matulova et al 2005).…”

Section: F Succinogenes Vs Other Speciesmentioning

confidence: 99%

Ecological and physiological characterization shows that Fibrobacter succinogenes is important in rumen fiber digestion — Review

2008

View full text Add to dashboard Cite

Fibrobacter succinogenes is a major cellulolytic species in the rumen. On the basis of molecular data, this species can be classified into four phylogenetic groups. Recently gathered ecological and physiological data have revealed the importance of this species, particularly phylogenetic group 1, in rumen fiber digestion. These data indicate that group 1 should be the focus of future efforts to maximize the fibrolytic function of the rumen.

show abstract

Analysis of bacterial phospholipid markers and plant monosaccharides during forage degradation by Ruminococcus flavefaciens and Fibrobacter succinogenes in co-culture

Cited by 24 publications

References 35 publications

Effect of Exogenous Fibrolytic Enzyme Application on the Microbial Attachment and Digestion of Barley Straw In vitro

Effect of Exogenous Fibrolytic Enzyme Application on the Microbial Attachment and Digestion of Barley Straw In vitro

Structural analysis of the carbohydrate components of the outer membrane of the lipopolysaccharide‐lacking cellulolytic ruminal bacterium Fibrobacter succinogenes S85

Ecological and physiological characterization shows that Fibrobacter succinogenes is important in rumen fiber digestion — Review

Contact Info

Product

Resources

About