~~ ~~ ~ ~~~Marker components of the phospholipids of Ruminococcus flavefaciens and Fibrobacter succinogenes were identified for studies on the degradation of forage by these bacteria growing in mixed culture. The principal fatty acid methyl esters and dimethyl acetals detected varied between strains and were influenced by the addition of a mixture of higher volatile fatty acids and vitamins to the medium, but these effects were small compared to the differences between the species. When two strains of R. flavefaciens were grown on a mixture of clover and ryegrass, and on barley straw in the presence or absence of two strains of F. succinugenes, the solubilization of plant material tended to be lowered by the presence of F. succinugenes. R.flavefaciens was the predominant bacterium among colonies recovered from roll tubes, and the phospholipids were primarily those of R. flavefaciens. Analysis of the culture supernatant liquids showed that F. succinogenes produced greater amounts of free and bound xylose from both clover and straw than did R. flauefaciens. With both forages, cultures containing the two species produced more soluble free arabinose, and less soluble-bound arabinose, than either species grown alone.
Microbial plasmalogen aldehydes (detected as dimethyl acetals, DMA) have been used to compare microbial populations associated with clover and barley straw incubated in nylon mesh bags in the rumen of a cow. The results suggest that the populations involved in the digestion of these substrates differ substantially and that population changes occur as digestion proceeds: these conclusions were supported by electron‐microscopic observations. Analysis of DMA suggested that populations associated with the particles of straw and clover differed more markedly than the corresponding populations in the liquid phase. When straw was pre‐incubated with the rumen cellulolytic bacterium Ruminococcus flavefaciens strain 17, the DMA characteristic of this bacterium were present at increased levels during subsequent incubation of the straw in the rumen, though the R. flavefaciens DMA tended to contribute a smaller proportion of the total DMA as the incubation time in the rumen was increased from 24 to 72h.
This study investigated the long term adaptation of a ruminal bacterium to growth on four different plant cell wall substrates. No significant increase in degradation was detected for lucerne, barley straw or weeping lovegrass after 23 serial subcultures of the cellulolytic rumen bacterium Ruminococcus flavefaciens strain 17 on each of these substrates. Significantly increased substrate degradation by R. flavefaciens strain 17 was however observed after 23 subcultures on perennial ryegrass. The increase in dry matter solubilisation (from 24.3 to 39.5% in 24 h incubation and from 52.3 to 61% in 72 h) was at least partially due to an increase in solubilisation of xylose, glucose and arabinose. Enhanced growth of the adapted strains occurred on this substrate. Significant increases in xylanase and beta-xylosidase specific activities were detected but no effect was detected on xylanase profiles in zymogram analyses. Similar responses were observed for two cultures originally derived from single-colony re-isolates. The most likely explanation for the observed adaptation involves selection for mutations affecting the regulation of xylanolytic enzymes.
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