Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction

Current Perspectives on Printing Technology for Biomedical Applications

“…For instance, multiple cell types such as mesenchymal stem cells, fibroblast and endothelial cells were patterned onto hydrogels through a layer-by layer 4D-bioprinting system. The cell migration and aggregation process results in vasculature formation which was confirmed by endothelialspecific gene expression [10]. Recently, soybean oil epoxidized acrylate was used as an ink for fabricating 3D-bioprinted biomedical scaffolds to elucidate biocompatibility and cytocompatibility with human bone marrow mesenchymal stem cells.…”

Emergence of Bioprinting in Tissue Engineering: A Mini Review

“…17 In this case, artificial tissues are seeded by either printing primary cells with supporting cells 19,20 or printing progenitors or stem cells for further differentiation. 21,22 Complexity in bioprinting may be increased by direct printing of primary cells. Multiple cell types incorporated into same or different hydrogel systems need to be printed in parallel and many bioinks need to be prepared for each print.…”

“…In other study, Hong et al 21 demonstrated the cellular behavior in micro-patterned hydrogels activated by maturation with multiple cell types through a 4D bioprinting system resulting in vascularization. 21 Further, for bone tissues, Pati et al 56 investigated the 4D bioprinting of hard-tissue bio constructs by printing grid pattern followed by coating with human nasal inferior turbinate tissue-derived mesenchymal stem cells (hTMSCs) for graft-mineralization.…”

“…21 Further, for bone tissues, Pati et al 56 investigated the 4D bioprinting of hard-tissue bio constructs by printing grid pattern followed by coating with human nasal inferior turbinate tissue-derived mesenchymal stem cells (hTMSCs) for graft-mineralization. Additionally, this printed bone tissue bio construct matured after a designed culture period and the decellularized bio construct demonstrated improved osteoinductive and osteoconductive performance in vitro and in vivo.…”

Hydrogels for Cell Encapsulation and Bioprinting

“…[12] Various types of hydrogels with different mechanisms for crosslinking have been used for bioprinting. Some of these hydrogels include collagen type I [13][14][15], collagen/fibrin [15,16], fibrin [15,17], Extracel TM Hydrogel [15], hyaluronic acid (HA) [1,15,18], hyaluronan-based hydrogels [19,20], hyaluronic acid and dextranbased semi-interpenetrating networks (semi-IPN) [21], tyramine-substituted hyaluronic acid (TS-NaHy) [15], Corgel TM [15], methylcellulose-hyaluronan (MC-HA) [15], chitosan [15], chitosan/collagen [15], methacrylated gelatin (GelMA) [22], polyethylene glycol diacrylate (PEGDA) [1,15], agarose [15,23], alginate [7,15,[24][25][26], alginate/gelatin [15,27], PEG [1], polyacrylamide-based hydrogels [28], and NovoGel [29]. Many factors need to be considered when selecting a hydrogel for bioprinting such as cytotoxicity, gelation time, extent of swelling, viscosity, printability, and hydrogel stability [15].…”