Hydrogels for Cell Encapsulation and Bioprinting

Shariati, Seyed Ramin Pajoum; Moeinzadeh, Seyedsina; Jabbari, Esmaiel

doi:10.1007/978-3-319-21386-6_4

Cited by 3 publications

References 149 publications

(214 reference statements)

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Cell Encapsulation Systems Toward Modular Tissue Regeneration: From Immunoisolation to Multifunctional Devices

Correia

Nadine

Mano

2020

Adv Funct Materials

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In the primordial cell encapsulation systems, the main goal was to treat endocrine diseases avoiding the action of the immune system. Although lessons afforded by such systems were of outmost importance for the demands of Tissue Engineering and Regenerative Medicine, the paradigm has recently completely changed. If before the most important feature was to mask the encapsulated cells from the immune system, now it is known that the synergetic interplay between immune cells and the engineered niche is responsible by an adequate regenerative process. Combined with such immuno-awareness, novel or non-conventional emerging techniques are being proposed developed the new generation of cell encapsulation systems, namely layer-by-layer, microfluidics, superhydrophobic surfaces, and bioprinting technologies.Alongside with the desire to create more realistic cell encapsulation systems, cell-laden hydrogels are being explored as building blocks for bottom-up strategies, within the concept of modular tissue engineering. The idea is to use the well-established cell friendly environment provided by hydrogels, and create more close-to-native systems owning high heterogeneity, while providing multifunctional and adaptive inputs.

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Cell Encapsulation Systems Toward Modular Tissue Regeneration: From Immunoisolation to Multifunctional Devices

Correia

Nadine

Mano

2020

Adv Funct Materials

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Bioprinting in Pharmaceuticals

Dixit,

Singh,

Das

et al. 2023

Additive Manufacturing in Pharmaceuticals

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Comparative Study of Ultrasonication-Induced and Naturally Self-Assembled Silk Fibroin-Wool Keratin Hydrogel Biomaterials

Xue

Vuong

et al. 2016

IJMS

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This study reports the formation of biocompatible hydrogels using protein polymers from natural silk cocoon fibroins and sheep wool keratins. Silk fibroin protein contains β-sheet secondary structures, allowing for the formation of physical cross-linkers in the hydrogels. Comparative studies were performed on two groups of samples. In the first group, ultrasonication was used to induce a quick gelation of a protein aqueous solution, enhancing the ability of Bombyx mori silk fibroin chains to quickly entrap the wool keratin protein molecules homogenously. In the second group, silk/keratin mixtures were left at room temperature for days, resulting in naturally-assembled gelled solutions. It was found that silk/wool blended solutions can form hydrogels at different mixing ratios, with perfectly interconnected gel structure when the wool content was less than 30 weight percent (wt %) for the first group (ultrasonication), and 10 wt % for the second group (natural gel). Differential scanning calorimetry (DSC) and temperature modulated DSC (TMDSC) were used to confirm that the fibroin/keratin hydrogel system was well-blended without phase separation. Fourier transform infrared spectroscopy (FTIR) was used to investigate the secondary structures of blended protein gels. It was found that intermolecular β-sheet contents significantly increase as the system contains more silk for both groups of samples, resulting in stable crystalline cross-linkers in the blended hydrogel structures. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) were used to analyze the samples’ characteristic morphology on both micro- and nanoscales, which showed that ultrasonic waves can significantly enhance the cross-linker formation and avoid phase separation between silk and keratin molecules in the blended systems. With the ability to form cross-linkages non-chemically, these silk/wool hydrogels may be economically useful for various biomedical applications, thanks to the good biocompatibility of protein molecules and the various characteristics of hydrogel systems.

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Hydrogels for Cell Encapsulation and Bioprinting

Cited by 3 publications

References 149 publications

Cell Encapsulation Systems Toward Modular Tissue Regeneration: From Immunoisolation to Multifunctional Devices

Cell Encapsulation Systems Toward Modular Tissue Regeneration: From Immunoisolation to Multifunctional Devices

Bioprinting in Pharmaceuticals

Comparative Study of Ultrasonication-Induced and Naturally Self-Assembled Silk Fibroin-Wool Keratin Hydrogel Biomaterials

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