Carbon Nanotube Atomic Force Microscopy with Applications to Biology and Electronics

Asis, Edward D. de; Li, You; Austin, Alex J.; Leung, Joseph; Nguyen, Cattien V.

doi:10.1007/978-3-642-03535-7_5

Cited by 3 publications

(3 citation statements)

References 85 publications

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“…We further demonstrated that the ends of these solubilized nanotubes remained active and could react with protein molecules. Nanotubes have been utilized in various biological applications such as biological sensor devices − and scanning probe microscopes. − The fluorescence labeling of nanotubes will allow a wider range of applications, especially in optics-based single-molecule devices.…”

Section: Discussionmentioning

confidence: 99%

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Fluorescence Labeling of Carbon Nanotubes and Visualization of a Nanotube−Protein Hybrid under Fluorescence Microscope

et al. 2011

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Biological applications of carbon nanotubes have been hampered by the inability to visualize them using conventional optical microscope, which is the most common tool for the observation and measurement of biological processes. Recently, a number of fluorescence labeling methods for biomolecules and various fluorescence probes have been developed and widely utilized in biological fields. Therefore, labeling carbon nanotubes with such fluorophores under physiological conditions will be highly useful in their biological applications. In this Article, we present a method to fluorescently label nanotubes by combining a detergent and a fluorophore commonly used in biological experiments. Fluorophores carrying an amino group (Texas Red hydrazide or BODIPY FL-hydrazide) were covalently attached to the hydroxyl groups of Tween 20 using carbonyldiimidazole. Fluorescence microscopy demonstrated that nanotubes were efficiently solubilized and labeled by this fluorescently labeled detergent. By using this technique, we also demonstrated multicolor fluorescence imaging of a nanotube-protein hybrid.

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Section: Discussionmentioning

confidence: 99%

“…Nanotubes have been utilized in various biological applications such as biological sensor devices [1][2][3][4] and scanning probe microscopes. [27][28][29][30][31] The fluorescence labeling of nanotubes will allow a wider range of applications, especially in optics-based single-molecule devices.…”

Section: ' Conclusionmentioning

confidence: 99%

Fluorescence Labeling of Carbon Nanotubes and Visualization of a Nanotube−Protein Hybrid under Fluorescence Microscope

et al. 2011

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“…AFM also has broad usage in the field of nanobiosciences that merge biology-, chemistry-and physics-based applications [de Asis et al, 2010]. Generally AFM does not require special treatments of the specimen prior to imaging that are often required for other specialized microscopy techniques.…”

Section: Discussionmentioning

confidence: 99%

Relating Centromeric Topography in Fixed Human Chromosomes to α-Satellite DNA and CENP-B Distribution

Khan

Chisholm

Tadayyon

et al. 2013

Cytogenet Genome Res

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Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of α-satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and α-satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes.

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Carbon Nanotube Tips in Atomic Force Microscopy with Applications to Imaging in Liquid

Asis

Leung

Nguyen

2012

Atomic Force Microscopy in Liquid

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Carbon Nanotube Atomic Force Microscopy with Applications to Biology and Electronics

Cited by 3 publications

References 85 publications

Fluorescence Labeling of Carbon Nanotubes and Visualization of a Nanotube−Protein Hybrid under Fluorescence Microscope

Fluorescence Labeling of Carbon Nanotubes and Visualization of a Nanotube−Protein Hybrid under Fluorescence Microscope

Relating Centromeric Topography in Fixed Human Chromosomes to α-Satellite DNA and CENP-B Distribution

Carbon Nanotube Tips in Atomic Force Microscopy with Applications to Imaging in Liquid

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