Bioprinting 101: Design, Fabrication, and Evaluation of Cell-Laden 3D Bioprinted Scaffolds

Deo, Kaivalya A.; Singh, Kanwar Abhay; Peak, Charles W.; Alge, Daniel L.; Gaharwar, Akhilesh K.

doi:10.1089/ten.tea.2019.0298

Cited by 130 publications

(101 citation statements)

References 227 publications

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“…Among these techniques, the extrusion-based bioprinting has become more popular (Fig. 5 c) because hydrogel precursors having low‐shear viscosities can be used for printing and it is able to deposit high cell densities, similar to the target tissue structure [ 202 ]. Although bioprinting techniques have the same principles of the methods explained in previous “ 3D printing technologies based on stimulation used ” section, they may have some differences due to the need for adaptation not to adversely influence the living cells.…”

Section: D Printing Of Bone Scaffold Based On Cad Modelmentioning

confidence: 99%

Challenges on optimization of 3D-printed bone scaffolds

Bahraminasab

2020

BioMed Eng OnLine

145

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Background Bones in human body are prone to damage due to different causes such as fractures, diseases, and infections. Nevertheless, they have a remarkable capacity to repair and heal themselves after trauma and illness. Large defects, however, are never completely reinstated because their sizes are beyond the limit up to which the bones can repair [1]. In these conditions, therefore, a medical remedy is required to stabilize, align and support the damaged bone region to restore the lost function. Bone autografts are considered the gold standard treatment. However, they have a number of shortcomings including the limited sources and donor site morbidity. Allografts also have the risk of immune rejection and disease transmission [2, 3]. Therefore, the research has headed for other solutions via tissue engineering. Bone tissue engineering provides three-dimensional (3D)

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Section: D Printing Of Bone Scaffold Based On Cad Modelmentioning

confidence: 99%

Challenges on optimization of 3D-printed bone scaffolds

Bahraminasab

2020

BioMed Eng OnLine

145

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“…Note that the porous structure of the matrixes led to the migration of osteoblasts inside the scaffold and differentiate into the bone that terminated to better and accelerated healing process. 40 Encapsulation of the cells in printable bio-inks enables homogeneous and uniform cell seeding and survive the attachment-dependent cells by mimicking the native ECM. 25 Additionally, it can provide a highly hydrated tissue-like environment for cells and improve new bone formation.…”

Section: Bone Defects Bone Formation and Bone Metabolicmentioning

confidence: 99%

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Ghorbani

Zhong

et al. 2020

J of Applied Polymer Sci

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Although many efforts have been made to regenerate the bone lesions, existing challenges can be mitigated through the development of tissue engineering scaffolds. However, the weak control on the microstructure of constructs, limitation in preparation of patient-specific and multilayered scaffolds, restriction in the fabrication of cell-laden matrixes, and challenges in preserving the drug/growth factors' efficacy in conventional methods have led to the development of bioprinting technology for regeneration of bone defects. So in this review, conventional 3D printers are classified, then the priority of the different types of bioprinting technologies for the preparation of the cell/growth factor-laden matrixes are focused. Besides, the bio-ink compositions, including polymeric/hybrid hydrogels and cell-based bio-inks are classified according to fundamental and recent studies. Herein, different effective parameters, such as viscosity, rheological properties, cross-linking methods, biodegradation biocompatibility, are considered. Finally, different types of cells and growth factors that can encapsulate in the bio-inks to promote bone repair are discussed, and both in vitro and in vivo achievement are considered. This review provides current and future perspectives of cell-laden bioprinting technologies. The restrictions and challenges are identified, and proper strategies for the development of cell-laden matrixes and high-performance printable bio-inks are proposed.

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“…3D printing is an additive manufacturing technique to obtain solid object via layer‐by‐layer deposition of ink. This approach is useful for generating tissue engineered grafts or in vitro models for bioengineering (Ashammakhi et al, 2019; Chimene et al, 2020; Deo, Singh, Peak, Alge, & Gaharwar, 2020; Peak, Singh, Ma, Chen, & Gaharwar, 2019). For example, Evans and co‐workers formed MOF‐thermoplastic polymer composites using ZIF‐8 and PLA via extrusion‐based additive manufacturing technique.…”

Section: Biomedical Applications Of 2d Mofsmentioning

confidence: 99%

Two‐dimensional metal organic frameworks for biomedical applications

Kumar

Balasubramaniam

Bhunia

et al. 2020

WIREs Nanomed Nanobiotechnol

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Two‐dimensional (2D) metal organic frameworks (MOFs), are an emerging class of layered nanomaterials with well‐defined structure and modular composition. The unique pore structure, high flexibility, tunability, and ability to introduce desired functionality within the structural framework, have led to potential use of MOFs in biomedical applications. This article critically reviews the application of 2D MOFs for therapeutic delivery, tissue engineering, bioimaging, and biosensing. Further, discussion on the challenges and strategies in next generation of 2D MOFs are also included. This article is categorized under: Nanotechnology Approaches to Biology > Nanoscale Systems in Biology

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Bioprinting 101: Design, Fabrication, and Evaluation of Cell-Laden 3D Bioprinted Scaffolds

Cited by 130 publications

References 227 publications

Challenges on optimization of 3D-printed bone scaffolds

Challenges on optimization of 3D-printed bone scaffolds

Bioprinting a cell‐laden matrix for bone regeneration: A focused review

Two‐dimensional metal organic frameworks for biomedical applications

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