Biogenic silver nanoparticles production and characterization from native stain of Corynebacterium species and its antimicrobial activity

Abstract

In the present study, synthesis, characterization, and the antibacterial activity of silver nanoparticles from native isolate of Corynebacterium glutamicum has been reported. Silver nanoparticles were synthesized by challenging the dried biomass of C. glutamicum with aqueous diamine silver ([Ag (NH3)2]+) containing 1 mM AgNO3. Synthesized silver nanoparticles (AgNPs) were characterized by ultraviolet–visible spectroscopy and energy-dispersive X-ray (EDX) spectroscopy analysis. Morphological study of silver nanoparticles was carried out using transmission electron microscopy (TEM) and scanning electron microscope (SEM). The spherical morphology of silver nanoparticles was confirmed from SEM image. The TEM image showed the average particle size of silver nanoparticles was about 15 nm. Silver nanoparticles synthesized from C. glutamicum were found to have enhanced antimicrobial activity against selected pathogenic strains. Silver nanoparticles from pure strains of Corynebacterium species was done by many investigators, but as per the present literature, this is the first report on the production of silver nanoparticles using a native strain of Corynebacterium.

Advances in Biogenic Nanoparticles and the Mechanisms of antimicrobial Effects

“…There are many sources of NP synthesis, but the green approaches are gaining popularity. Plant parts such as, the leaf, the bark, the flower, the peel, and the seed and microorganisms such as fungi, bacteria, algae, yeast, actinomycetes, and enzymes offer clean, eco-friendly, non-toxic machinery for their synthesis, which is compatible with pharmaceutical and cosmeceutical applications [9][10][11] . These NMs have long been documented to exhibit microbiocidal, microbiostatic actions and serve as potential antibacterial agents in medical and industrial applications [12] .…”

Green Intelligent Nanomaterials by Design (Using Nanoparticulate/2D-Materials Building Blocks) Current Developments and Future Trends

“…Green synthesis of NPs involving microorganisms and plants is noted to be safe, inexpensive, and environment-friendly as they absorb and accumulate inorganic metal ions from their surroundings leading to an unexplored field of useful research [186][187][188][189]. A number of biotechnological applications including bioremediation and bioleaching are possible to implement by exploiting the efficacy of the microorganisms to interact with the environment via their lipid-based amphipathic membranes enabling a variety of oxidation-reduction mechanisms occurring and promoting these biochemical conversions [190][191][192][193][194].…”

“…Generally, biological entities with a potential to accumulate heavy metals offer better chance of synthesizing metal NPs [200]. In case of microorganisms, optimization of parameters like nutrients, light, pH, temperature, mixing speed, and buffer strength used in their culture could significantly enhance the enzyme activity [188,201,202].…”

Nano Dimensional (1-20nm) Silver Nanoparticles: Stem Extract of Capparis decidua (FORSK.) EDGEW Mediated Synthesis and its Characterization-A Lab to Land Approach

“…The findings reported that extracellular synthesis using plant extracts has been considered better as compared to intracellular (Makarov et al, 2014) because it abolish the extraction and purification methods. Green synthesis have been reported in Magnifera indica (Sandeep et al, 2017), Corynebacterium species (Gowramma et al, 2015).…”