Beyond osmolytes and transcription factors: drought tolerance in plants via protective proteins and aquaporins

Hussain, Syed Sarfraz; Iqbal, M. T.; Arif, Muhammad; Amjad, Muhammad

doi:10.1007/s10535-011-0104-9

Cited by 41 publications

(12 citation statements)

References 221 publications

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“…Contrariamente ao observado em tomate, plantas de Arabidopsis super-expressando uma TIP de soja se mostraram mais suscetíveis à desidratação (Wang et al 2011). Segundo Hussain et al (2011), existem atualmente interpretações divergentes dos resultados obtidos com plantas transgênicas. Para alguns autores, o aumento nos níveis de aquaporina melhora a capacidade das plantas em lidar com o estresse hídrico, enquanto outros acreditam que as plantas evitam perdas excessivas de água ao "desligar" as aquaporinas durante a desidratação.…”

Section: Aquaporinas E Resposta a Estresses Ambientaisunclassified

Aquaporinas: de canais de água a transportadores multifuncionais em plantas

Gaspar

2011

Rev. bras. Bot.

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-(Aquaporins: from water channels to multifunctional transporters in plants). From the functional identification of the first plant aquaporin, in 1993, up to today, a lot of information about the structure, location and function of different members of this multigene family of membrane proteins was provided by the scientific community. Initially regarded as "simple water channels", the aquaporins have been shown to transport gases and small neutral solutes such as glycerol, urea and silicon, transforming the concept of transmembrane transport. Due to the redundancy of aquaporin genes and their distribution in all organs and plant tissues, these proteins have been considered essential in maintaining vital functions in plants such as water and nutrient uptake in roots, seed germination, photosynthesis, transpiration and reproduction. This work presents a review on molecular and functional characterization of aquaporins and their relevance for plant development and in response to environmental stresses. Despite advances in research on plant aquaporins, there are few studies with native species from tropical regions and therefore there is a vast field of research to be explored, given the differences in water availability, the occurrence of various environmental stresses in tropical biomes, and the mechanisms employed by plants of these regions to be adapted to the wide variety of environmental conditions. Key words -major intrinsic protein (MIP), solute transport, stress response, water balance RESUMO -(Aquaporinas: de canais de água a transportadores multifuncionais em plantas). Da identificação funcional da primeira aquaporina de plantas, em 1993, aos dias de hoje, uma grande quantidade de informações sobre a estrutura, a localização e a função dos diferentes membros desta família multigênica de proteínas de membrana foi disponibilizada pela comunidade científica. inicialmente consideradas como "simples canais de água", as aquaporinas se mostraram capazes de transportar gases e pequenos solutos neutros, como glicerol, uréia e silício, revolucionando o conceito de transporte transmembrana. Devido à redundância dos genes de aquaporinas e à sua distribuição em todos os órgãos e tecidos vegetais, essas proteínas têm sido consideradas essenciais na manutenção de funções vitais, como absorção de água e nutrientes em raízes, germinação de sementes, fotossíntese, transpiração e reprodução. O presente trabalho faz uma revisão sobre a caracterização funcional e molecular das aquaporinas e sua importância no desenvolvimento da planta e na resposta a estresses ambientais. Apesar dos avanços nas pesquisas com aquaporinas de plantas, existem poucos estudos com espécies nativas de regiões tropicais havendo, portanto, vasto campo de pesquisa a ser explorado, tendo em vista as diferenças na disponibilidade de água, a ocorrência de estresses ambientais diversos nos biomas tropicais e os mecanismos empregados pelas plantas dessas regiões para se adaptar à grande variedade de condições ambientais.Palavras-chave -balanço híd...

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Section: Aquaporinas E Resposta a Estresses Ambientaisunclassified

Aquaporinas: de canais de água a transportadores multifuncionais em plantas

Gaspar

2011

Rev. bras. Bot.

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“…Eight sHSP genes showed more obvious transcriptional changes when exposed to temperatures of 42 °C than those exposed to 200 µM ABA. The sHSPs, as well as other HSPs, are proved to play a significant role in plant stress tolerance, such as extreme temperatures (Campbell et al 2001, Jiang et al 2009, Grigorova et al 2011, dehydration (Campbell et al 2001, Sato and Yokoya 2008, Hussain et al 2011, ABA treatment (Campbell et al 2001). In conclusion, all sHSP genes showed multiple cellular responses to environmental stress, during various developmental processes, and in different reproductive organs.…”

Section: Discussionmentioning

confidence: 91%

Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris

Peng¹,

Liu²,

Wang³

2012

Biol. plant.

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Small heat shock proteins (sHSPs) are crucial components of the plant response to heat shock. We identified and analyzed eight sHSP genes of Capsella bursa-pastoris to better understand the ability of this species to adapt. Eight genes were initially cloned and sequenced from the mature embryo cDNA pool. They belong to the cytosolic I (CI), cytosolic II (CII), and cytosolic III (CIII) subfamilies. One CI sHSP gene was homologous to that of C. rubella. Sequence analysis using 3'RACE revealed that there are two or more variable 3'-untranslated regions (UTRs) in these sHSP transcripts. The transcriptional levels of the eight sHSP genes were analyzed in different organs and developmental stages via qRT-PCR. Eight genes were significantly up-regulated in young leaves exposed to heat stress at 42 °C, and also showed differential responses to ABA treatment. We also compared expression of these genes with corresponding Arabidopsis sHSP genes and found some differences between the two species.

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“…Variation in SP content is a water stress tolerance response 37 related to synthesis, accumulation or degradation. The SP accumulation at 1% of PEG compared to control (without PEG stress) is probably related to the synthesis of hydrophilins 38 , aquaporins 39 , dehydrins 40 , antioxidant enzymes 41 , and chaperonins 42 , among others. Plants can synthesize this type of protein to neutralize oxidative stress and osmotic stress 41 , 43 .…”

Section: Discussionmentioning

confidence: 99%

In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

Martínez-Santos

Cruz‐Cruz

Spinoso-Castillo

et al. 2021

Sci Rep

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Drought-induced water stress affects the productivity of the Vanilla planifolia Jacks. ex Andrews crop. In vitro culture technique is an effective tool for the study of water stress tolerance mechanisms. This study aimed to evaluate the morphological, physiological and biochemical response of V. planifolia under in vitro water stress conditions induced with polyethylene glycol (PEG). In vitro regenerated shoots of 2 cm in length were subjected to different concentrations of PEG 6000 (0, 1, 2 and 3% w/v) using Murashige and Skoog semi-solid culture medium. At 60 days of culture, different growth variables, dry matter (DM) content, chlorophyll (Chl), soluble proteins (SP), proline (Pro), glycine betaine (GB), stomatal index (SI) and open stomata (%) were evaluated. Results showed a reduction in growth, Chl content, SP, SI and open stomata (%) with increasing PEG concentration, whereas DM, Pro and GB contents rose with increasing PEG concentration. In conclusion, PEG-induced osmotic stress allowed describing physiological and biochemical mechanisms of response to water stress. Furthermore, the determination of compatible Pro and GB osmolytes can be used as biochemical markers in future breeding programs for the early selection of water stress tolerant genotypes.

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Beyond osmolytes and transcription factors: drought tolerance in plants via protective proteins and aquaporins

Cited by 41 publications

References 221 publications

Aquaporinas: de canais de água a transportadores multifuncionais em plantas

Aquaporinas: de canais de água a transportadores multifuncionais em plantas

Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris

In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

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