Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris

Peng, Tao; Liu, L.; Wang, J. B.

doi:10.1007/s10535-012-0239-3

Cited by 6 publications

(1 citation statement)

References 46 publications

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“…However, paralogous sHSP genes showed different expression levels; for example, the transcriptional level of Br22.0 MT in different organs and across embryonic stages was at least three times higher than that of Br18.0 MT. Tao et al (2012) compared the expression of two paralogous C-II sHSP genes (CbHSPCIIa and CbHSPCIIb) and likewise found that the expression of CbHSPCIIa was approximately five times higher than that of CbHSPCIIb in different organs and across embryonic stages. Br18.0A I, Br18.0B I, and Br17.1 were not expressed in any organs.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Peng

Guo²,

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (sHSPs) are essential for the plant's normal development and stress responses, especially the heat stress response. The information regarding sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify sHSP genes in this species. We identified 26 non-redundant sHSP genes distributed on all chromosomes, except chromosome A7, with one additional sHSP gene identified from an expressed sequence tag library. Chinese cabbage was found to contain more sHSP genes than Arabidopsis. The 27 sHSP genes were classified into 11 subfamilies. We identified 22 groups of sHSP syntenic orthologous genes between Chinese cabbage and Arabidopsis. In addition, eight groups of paralogous genes were uncovered in Chinese cabbage. Protein structures of the 27 Chinese cabbage sHSPs were modeled using Phyre2, which revealed that all of them contain several conserved β strands across different subfamilies. In general, gene structure was conserved within each subfamily between P. Tao et al.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Peng

Guo²,

et al. 2015

Genet. Mol. Res.

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Comparative analysis of the variable 3′ UTR and gene expression of the KIN and KIN-homologous LEA genes in Capsella bursa-pastoris

Peng

Huang

et al. 2012

Plant Cell Rep

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As the crucial members of the cold-regulated (COR) gene family, KIN genes are involved in diverse abiotic stress responses in plants. In the present study, KIN genes from the widespread plant Capsella bursa-pastoris were identified and analyzed to better understand the powerful adaptation of this species. Two KIN genes were cloned and sequenced by 3' RACE. As some COR genes are homologous to LEA genes, three KIN-homologous LEA genes were also identified. We deduced the amino acid sequences of the five proteins to estimate their phylogenetic relationships, and grouped them into three subfamilies (CI, CII, and CIII). Variable 3' UTRs were found in CI, CII, and CIII genes. Using qPCR, we evaluated the transcriptional levels of the five genes in different organs and embryonic stages. Two CI genes were exclusively expressed in early embryos and flowers. The CII and CIII genes showed obvious up-regulation in young leaves after heat stress, cold stress, and ABA treatment. Two of the CI genes, however, rarely responded to those stresses in young leaves. In contrast, all five genes showed differential responses in flowers when C. bursa-pastoris plants were sprayed with ABA. Furthermore, the expression of these genes in C. bursa-pastoris was compared to that of the corresponding Arabidopsis genes, and similar gene expression profiles were found in both species. Our findings suggest that these five genes play different roles in development and the responses to abiotic stresses in C. bursa-pastoris. Key message We characterized two KIN and three KIN-homologous LEA genes, and analyzed their variable 3'UTR and organ-specific, embryo-developmental, stress-induced gene expression in Capsella bursa-pastoris.

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Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants

Cvjetko¹,

Balen²,

Štefanić³

et al. 2014

Biologia plant.

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Characterization of eight cytosolic sHSP genes and their expression in Capsella bursa-pastoris

Cited by 6 publications

References 46 publications

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Comparative analysis of the variable 3′ UTR and gene expression of the KIN and KIN-homologous LEA genes in Capsella bursa-pastoris

Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants

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