Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants

Cvjetko, Petra; Balen, Biljana; Štefanić, Petra Peharec; Debogović, Lea; Pavlica, Mirjana; Klobučar, Göran

doi:10.1007/s10535-013-0362-9

Cited by 17 publications

(7 citation statements)

References 44 publications

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“…3C), which induced DNA damage in leaves by reacting with cell components, resulting in cell death (Potters et al, 2010). This result agrees with the findings of Cvjetko et al (2014). However, application of Mg significantly inhibited DNA damage, most likely because of the accumulation of Pro and GB, and the enhanced antioxidant enzyme activity (Figure 1, Figure 2), which mitigates the effects of ROS on leaf nuclear DNA (Gichner et al, 2008).…”

Section: Discussionsupporting

confidence: 89%

Mitigation of adverse effects of heat stress on Vicia faba by exogenous application of magnesium

Siddiqui

Alamri

Al-Khaishany

et al. 2018

Saudi Journal of Biological Sciences

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The effects of magnesium (Mg) supplementation on the growth performance, oxidative damage, DNA damage, and photosynthetic pigment synthesis, as well as on the activity level of carbonic anhydrase (CA), ribulose-1,5-bisphosphate carboxylase (Rubisco), and antioxidant enzymes were studied in Vicia faba L. plants exposed to heat stress (HS) and non-heat-stress (non-HS) conditions. Seeds were grown in pots containing a 1:1 mixture of sand and peat, with Mg treatments. The treatments consisted of (i) 0 Mg and non-HS (ambient temperature; control); (ii) 50 mM Mg; (iii) HS (38 °C); and (iv) 50 mM Mg and HS (38 °C). HS was imposed by placing potted plants in an incubator at 38 °C for 48 h. Growth attributes, total chlorophyll (Total Chl), and CA, and Rubisco activity decreased in plants subjected to HS, whereas accumulation of organic solutes [proline (Pro) and glycine betaine (GB)]; superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activity; DNA damage; electrolyte leakage (EL); and malondialdehyde (MDA) and hydrogen peroxide (H2O2) content all increased. Application of Mg, however, significantly enhanced further proline (Pro), glycinebetaine (GB), SOD, POD, and CAT activity, and decreased DNA damage, EL, and MDA and H2O2 concentrations. These results suggest that adequate supply of Mg is not only essential for plant growth and development, but also improves plant tolerance to HS by suppressing cellular damage induced by reactive oxygen species through the enhancement of the accumulation of Pro and GB, and the actions of antioxidant enzymes.

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Section: Discussionsupporting

confidence: 89%

Mitigation of adverse effects of heat stress on Vicia faba by exogenous application of magnesium

Siddiqui

Alamri

Al-Khaishany

et al. 2018

Saudi Journal of Biological Sciences

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“…This suggests crosstalk between HSP and ROS production, showing that, as signaling molecules, the ROS can induce HSPs, which corroborates previous reports [ 43 , 44 ]. Our results suggest the participation of DNA damage as well as energy metabolism reprogramming in the course of heat acclimation [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 97%

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Jiang²,

Qin³

et al. 2019

Genes

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Wheat, a major worldwide staple food crop, is relatively sensitive to a changing environment, including high temperature. The comprehensive mechanism of heat stress response at the molecular level and exploitation of candidate tolerant genes are far from enough. Using transcriptome data, we analyzed the gene expression profiles of wheat under heat stress. A total of 1705 and 17 commonly differential expressed genes (DEGs) were identified in wheat grain and flag leaf, respectively, through transcriptome analysis. Gene Ontology (GO) and pathway enrichment were also applied to illustrate the functions and metabolic pathways of DEGs involved in thermotolerance of wheat grain and flag leaf. Furthermore, our data suggest that there may be a more complex molecular mechanism or tighter regulatory network in flag leaf than in grain under heat stress over time, as less commonly DEGs, more discrete expression profiles of genes (principle component analysis) and less similar pathway response were observed in flag leaf. In addition, we found that transcriptional regulation of zeatin, brassinosteroid and flavonoid biosynthesis pathways may play an important role in wheat’s heat tolerance. The expression changes of some genes were validated using quantitative real-time polymerase chain reaction and three potential genes involved in the flavonoid biosynthesis process were identified.

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“…The level of GSH and/or the activity of enzymes involved in the glutathione redox cycle are inversely associated with the oxidative DNA damage (53,54). Moreover, heat exposure induces oxidative stress and DNA damage in mice, humans, plants and fish (55)(56)(57)(58)(59)(60), and heat acclimation led to a decreased accumulation of 8-OHdG markers in blood cells of navy boiler tenders exposed to high heat during work (61). Finally, increased DNA damage was observed in coral tissue explants exposed to elevated temperatures or to direct sunshine (20,62).…”

Section: Discussionmentioning

confidence: 99%

Thermal preconditioning in a reef-building coral alleviates oxidative damage through a BI-1 mediated antioxidant response

Majerová

Drury

2021

Preprint

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Global coral reef decline is driven by the breakdown of the coral-algal symbiosis during temperature stress. Corals can acclimatize to higher temperatures on intra-generational timescales, but the complex cellular processes that underlie this ability and its trade-offs are poorly understood. We show that preconditioning-based improvements in thermal tolerance in Pocillopora acuta are accompanied by host increases in glutathione reductase (GR) activity and expression, which support a reducing intracellular environment that facilitates reactive oxygen scavenging and prevents DNA damage. We found a strong correlation between GR and BI-1 (Bax-inhibitor 1) expression in heat-stressed preconditioned corals and discovered an antioxidant response element (ARE) in the GR promoter, suggesting BI-1 could regulate GR expression in corals through the Nrf2/ARE pathway. To fortify this link, we developed an siRNA-mediated gene knockdown protocol and targeted the coral BI-1 gene. BI-1 knock-down decreased glutathione reductase expression, glutathione reductase activity and increased oxidative DNA damage in heat-stressed preconditioned corals, showing that enhanced regulation of antioxidant response during acute heat stress is a key mechanism that prevents oxidative DNA damage after preconditioning. These results describe the manipulation of an important molecular cascade at the core of symbiosis maintenance under thermal stress and show that induced symbiosis stability does not impact DNA integrity.

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Dynamics of heat-shock induced DNA damage and repair in senescent tobacco plants

Cited by 17 publications

References 44 publications

Mitigation of adverse effects of heat stress on Vicia faba by exogenous application of magnesium

Mitigation of adverse effects of heat stress on Vicia faba by exogenous application of magnesium

Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Thermal preconditioning in a reef-building coral alleviates oxidative damage through a BI-1 mediated antioxidant response

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