Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Abstract

Wheat, a major worldwide staple food crop, is relatively sensitive to a changing environment, including high temperature. The comprehensive mechanism of heat stress response at the molecular level and exploitation of candidate tolerant genes are far from enough. Using transcriptome data, we analyzed the gene expression profiles of wheat under heat stress. A total of 1705 and 17 commonly differential expressed genes (DEGs) were identified in wheat grain and flag leaf, respectively, through transcriptome analysis. Gene Ontology (GO) and pathway enrichment were also applied to illustrate the functions and metabolic pathways of DEGs involved in thermotolerance of wheat grain and flag leaf. Furthermore, our data suggest that there may be a more complex molecular mechanism or tighter regulatory network in flag leaf than in grain under heat stress over time, as less commonly DEGs, more discrete expression profiles of genes (principle component analysis) and less similar pathway response were observed in flag leaf. In addition, we found that transcriptional regulation of zeatin, brassinosteroid and flavonoid biosynthesis pathways may play an important role in wheat’s heat tolerance. The expression changes of some genes were validated using quantitative real-time polymerase chain reaction and three potential genes involved in the flavonoid biosynthesis process were identified.

Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

“…In the present study, 23 metabolism-related SDEPs were identified and 21 of them were decreased in maca seedlings following HTS (Additional file 3). This agrees with previous transcriptomic data showing that a specific cluster of genes, which are involved in the metabolism of carbohydrates, amino acids and lipids, are significantly decreased to adapt to the reduced need for primary metabolic products under HTS [15,18]. Furthermore, 11 SDEPs involved in secondary metabolism were identified in the present study.…”

“…Therefore, the identification of proteins involved in HSRs is crucial to understand the molecular mechanisms of plant response strategies to HTS. To date, although several studies have investigated plant responses to HTS in tomato, grape, rice, and wheat using transcriptomic or proteomic approaches [8,[14][15][16][17][18], the molecular-level mechanisms underlying plant responses to HTS are still not fully understood, at least in herbal plants.…”

Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

“…In the present study, 27 metabolism-related SDEPs were identified and 25 of them were decreased in maca seedlings following HTS (Additional file 3). This is in line with previous transcriptomic data which showed that a specific cluster of genes, which are involved in the metabolism of carbohydrates, amino acids and lipids, are significantly decreased to adapt to the reduced need of primary metabolic products under HTS [6,11,14]. Furthermore, 13 SDEPs involved in secondary metabolism were identified in the present study.…”

Genome-Wide Development and Validation of Cost-Effective KASP Marker Assays for Genetic Dissection of Heat Stress Tolerance in Maize

“…In response to heat stress, plants accumulate different metabolites such as antioxidants, osmoprotectants, and HSPs that trigger multiple pathways involved in signal transduction, the scavenging of ROS, and maintaining cell membrane stability to improve damage and sustain cell homeostasis [ 49 ]. In the recent past, many studies have been identified and analyzed HSR genes in maize [ 12 , 13 , 14 , 15 , 16 , 17 , 18 ].…”