Comparative analysis of maca (Lepidium meyenii) proteome profiles reveals insights into response mechanisms of herbal plants to high-temperature stress

Abstract

Background High-temperature stress (HTS) is one of the main environmental stresses that limit plant growth and crop production in agricultural systems. Maca (Lepidium meyenii) is an important high-altitude herbaceous plant adapted to a wide range of environmental stimuli such as cold, strong wind and UV-B exposure. However, it is an extremely HTS-sensitive plant species. Thus far, there is limited information about gene/protein regulation and signaling pathways related to the heat stress responses in maca. In this study, proteome profiles of maca seedlings exposed to HTS for 12 h were investigated using a tandem mass tag (TMT)-based proteomic approach. Results In total, 6966 proteins were identified, of which 300 showed significant alterations in expression following HTS. Bioinformatics analyses indicated that protein processing in endoplasmic reticulum was the most significantly up-regulated metabolic pathway following HTS. Quantitative RT-PCR (qRT-PCR) analysis showed that the expression levels of 19 genes encoding proteins mapped to this pathway were significantly up-regulated under HTS. These results show that protein processing in the endoplasmic reticulum may play a crucial role in the responses of maca to HTS. Conclusions Our proteomic data can be a good resource for functional proteomics of maca and our results may provide useful insights into the molecular response mechanisms underlying herbal plants to HTS.

Integrated next-generation sequencing and comparative transcriptomic analysis of leaves provides novel insights into the ethylene pathway of Chrysanthemum morifolium in response to a Chinese isolate of chrysanthemum virus B

“…Total RNA from different leaf samples was extracted using the FastPure Plant Total RNA Isolation Kit (Vazyme) and genomic DNA contamination was cleaned with DNase I, as described previously [ 40 , 43 ]. cDNA synthesis and qRT-PCR were performed as described by Wang et al [ 43 ].…”

“…Total RNA from different leaf samples was extracted using the FastPure Plant Total RNA Isolation Kit (Vazyme) and genomic DNA contamination was cleaned with DNase I, as described previously [ 40 , 43 ]. cDNA synthesis and qRT-PCR were performed as described by Wang et al [ 43 ]. For each candidate gene, PCR was performed twice for each biological replicate and relative mRNA expression levels were calculated using the 2 –ΔΔCt method [ 44 ] based on three independent biological replicates.…”

Identification and Molecular Characterization of a Novel Carlavirus Infecting Chrysanthemum morifolium in China

“…Total RNA extraction and cDNA synthesis were performed, as described previously [6]. qPCR was carried out on a CFX96 Touch Deep Well Real-Time PCR Detection System (Bio-Rad, Pleasanton, CA, USA), as described by Wang et al [51]. N. benthamiana actin 2 (NbACT2) was used as an internal reference [46,52].…”