In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

Martínez-Santos, Eduardo; Cruz‐Cruz, Carlos A.; Spinoso-Castillo, José Luis; Bello-Bello, Jericó Jabín

doi:10.1038/s41598-021-02207-0

Cited by 19 publications

(7 citation statements)

References 63 publications

(83 reference statements)

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“…On the other hand, Liao et al [ 57 ] observed that mycorrhizae ( Glomus lamellosum and Glomus etunicatum ) at different doses (0, 60, 120, 180, and 240 spores per plant) and a 2-week period of drought stress had no significant effect on the protein content of C. migao . The increase in protein content could be a drought stress tolerance response and represent the synthesis of hydrophilins, aquaporins (AQP), dehydrins, antioxidant enzymes, and chaperonins as tolerance mechanisms against drought stress [ 58 ]. AMF symbiosis specifically induces the expression of genes and protein transporters associated with a drought stress tolerance response; these include AQPs, inorganic phosphorus transporters, and ammonium, nitrate, sulfur, zinc, and carbon transporters [ 7 , 59 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (Saccharum spp.) under Drought Stress Conditions

Spinoso-Castillo¹,

Moreno-Hernández²,

Mancilla-Álvarez³

et al. 2023

Plants

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The symbiotic associations between arbuscular mycorrhizal fungi (AMF) and plants can induce drought stress tolerance. In this study, we evaluated the effect of Glomus intraradices, a mycorrhizal fungus, on the ex vitro development and survival of sugarcane plantlets subjected to drought stress during the acclimatization stage of micropropagation. In vitro obtained sugarcane plantlets (Saccharum spp. cv Mex 69–290) were inoculated with different doses of G. intraradices (0, 100, and 200 spores per plantlet) during greenhouse acclimatization. Sixty days after inoculation, plantlets were temporarily subjected to drought stress. We evaluated the survival rate, total chlorophyll, total protein, carotenoids, proline, betaine glycine, soluble phenolic content, and antioxidant capacity every 3 days for 12 days. Symbiotic interaction was characterized by microscopy. Our results showed that the survival rate of inoculated plants was higher in 45% than the treatment without mycorrhizae. Total chlorophyll, protein, proline, betaine glycine content, and antioxidant capacity were increased in AMF inoculated plants. The soluble phenolic content was higher in non-inoculated plants than the treatment with mycorrhizae during the drought stress period. Microscopy showed the symbiotic relationship between plant and AMF. The early inoculation of 100 spores of G. intraradices per sugarcane plantlet during the acclimatization stage could represent a preconditioning advantage before transplanting into the field and establishing basic seedbeds.

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Section: Discussionmentioning

confidence: 99%

Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (Saccharum spp.) under Drought Stress Conditions

Spinoso-Castillo¹,

Moreno-Hernández²,

Mancilla-Álvarez³

et al. 2023

Plants

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“…Y finalmente desde un punto de vista social ya que el cultivo de la vainilla se puede beneficiar a los pequeños productores obteniendo grandes ingresos económicos a través de estrategias de planeación y transferencias de tecnología (Borbolla-Pérez et al, 2017). La vainilla al ser un cultivo clonal se encuentra fuertemente amenazada por factores de estrés tanto bióticos (Hongos; Colletotrichum orchidophilum (Charron et al, 2018), Colletotrichum gloeosporoides (Guevara-Suarez et al, 2022) y Fusarium (Ramírez-Mosqueda et al, 2019), como abióticos (sequía) (Iglesias-Andreu et al, 2021;Martínez-Santos et al, 2021). Esto es debido a la ausencia de diversidad genética que permita aumentar la probabilidad de supervivencia con la presencia de genes que respondan de manera favorable a situaciones de estrés que afecte a las plantas morfo-fisiológicamente, derivando en problemas como aborción floral y/o de frutos, cierre estomático, reducción de la tasa fotosintética, inhibición del crecimiento, reducción del área foliar, deshidratación severa, aumento en la susceptibilidad de patógenos y por último necrosis (Borbolla-Pérez et al, 2016).…”

Section: Características Del Cultivo Y Producción De Vanilla Planifoliaunclassified

“…Por lo que es en este sentido que el sistema de conservación in vitro por medio del cultivo de tejidos vegetales que se puede optar por la ruta de la conservación de germoplasma de vainilla con especial interés ante diferentes factores de producción, resistencia, tolerancia, etc. (Gantait, 2017;Martínez-Santos et al, 2021;Pastelín-Solano et al, 2019;.…”

Section: Conservación De Vanilla Planifoliaunclassified

Vanilla cultivation in Mexico: Typology, characteristics, production, agroindustrial prospective and biotechnological innovations as a sustainability strategy

et al. 2023

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Vanilla is the orchid of greatest economic interest to the world since the spice of the same name is obtained from it. Vanillin is obtained from its ripened pod, this compound is highly recognized and demanded by the gastronomic, pharmaceutical, and cosmetic industries for the sweet aroma and flavor it emanates. The objective of this work was to carry out a literature review to put into perspective the reader about the sustainability strategies in the vanilla industry in Mexico, as well as the innovations of propagation and in vitro conservation of Vanilla planifolia germplasm. Despite being originally from Mexico, this country ranks third in world production, preceding Madagascar, and Indonesia. This compilation of studies presents biotechnological techniques to increase vanilla production, as well as alternatives that can reduce the negative effects caused by the low genetic accumulation in this species, such as plant tissue culture, use of molecular markers for the study of somaclonal variation, asymbiotic seed germination under in vitro conditions. These techniques together with agro-industrial strategies can represent a sustainable alternative for vanilla production. In this article, strategies are suggested that allow those interested in vanilla production to make appropriate decisions, however, it is necessary to develop multidisciplinary studies that allow a global approach to the phenomena involved in the production and use of vanilla, as well as the resolution of the problems that this process faces.

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“…The carbohydrates are considered a significant component for the storage medium since the use of osmotically active substances can lower the osmotic potential of the substrate, modify the growth of the shoots and affect the storage time [ 51 ].…”

Section: Factors Affecting Sgs Of Shoot Cultures Of Temperate Fruit C...mentioning

confidence: 99%

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Benelli̇

Tarraf

İzgü

et al. 2022

Plants

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Plant genetic resources conservation may be a potential option for the improvement of agricultural crops through modern biotechnologies, and in vitro conservation is a tool available to safeguard plant biodiversity. Ex situ conservation of plant genetic resources using the in vitro procedures is in progress in many countries. The slow growth storage (SGS) technique is a valid in vitro approach to preserve several vegetatively propagated species by controlling the growth and development of plantlets, economizing storage space and labor and reducing costs. Moreover, SGS prolongs the timing between subcultures, lowers the risk of losing germplasm through handling errors, such as contamination problems, and decreases the risk of genetic instability due to the reduction in the number of subcultures. SGS is applied by considering different factors: temperature, light or darkness conditions, medium composition, including mineral or sucrose concentrations, and the presence/absence of plant growth regulators, osmotic agents and growth inhibitors. SGS protocols for some fruit species have been well defined, others require additional research. The present review focuses on the effect of several factors that influence the SGS of in vitro shoots derived from temperate and tropical fruit species during the last ten years.

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In vitro response of vanilla (Vanilla planifolia Jacks. ex Andrews) to PEG-induced osmotic stress

Cited by 19 publications

References 63 publications

Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (Saccharum spp.) under Drought Stress Conditions

Arbuscular Mycorrhizal Symbiosis Improves Ex Vitro Acclimatization of Sugarcane Plantlets (Saccharum spp.) under Drought Stress Conditions

Vanilla cultivation in Mexico: Typology, characteristics, production, agroindustrial prospective and biotechnological innovations as a sustainability strategy

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

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