In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Benelli̇, C.; Tarraf, Waed; İzgü, Tolga; Carlo, Anna De

doi:10.3390/plants11233188

Cited by 23 publications

(27 citation statements)

References 75 publications

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“…Como resultado, ocorre o prolongamento entre as subculturas, que resulta na redução de custos, pois ocorre economia de espaço, mão de obra e reagentes. Além disso, ocorre a diminuição de contaminações no manuseio e risco de instabilidade genética (BENELLI et al, 2022).…”

Section: Discussionunclassified

Conservação in vitro através do crescimento lento de Ananas lucidus Mill. (curauá)

Ferreira¹,

Costa²,

Silva³

et al. 2023

CLCS

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A flora amazônica possui uma grande variedade de espécies com potencial uso presente ou futuro. Dentre diversas espécies, o Ananas lucidus Mill. (curauá), família das Bromeliáceas, é uma planta fibrosa amazônica. Os indígenas utilizavam as folhas do curauá como fonte de fibras para a confecção de cordas, redes e cestos. Entretanto, o desmatamento da floresta, resulta na erosão genética de variantes genéticas do curauá, sendo necessário adotar estratégias de conservação desse material genético. O objetivo do presente trabalho foi desenvolver um protocolo de conservação in vitro para o curauá, visando o crescimento lento. Os explantes de curauá foram inoculados em meio MS em duas salas distintas. Sala 1: temperatura de 18 ± 1°C, três diferentes irradiâncias de luz LED branca: 35, 45 e 75 μmol.m-2.s-1. Sala 2: temperatura de 25 ± 1°C, irradiância de luz fluorescente branca fria: 25 μmol.m-2.s-1. Na temperatura de 18 ± 1°C, a sobrevivência foi acima de 70% durante os 480 dias avaliados. Na temperatura de 25 ± 1°C, a irradiância de 25 μmol.m-2.s-1 obteve porcentagem de sobrevivência de 100% até o 6º mês (180 dias). Na avaliação da altura, na primeira avaliação, as irradiancias de 35, 45 e 75 μmol.m-2.s-1, a 18 ± 1ºC, a altura média das plântulas foi de 1,38; 1,40 e 1,75 cm, respectivamente. Não houve diferença estatística significativa entre as diferentes irradiâncias. Nas avaliações seguintes, a altura média aumenta, mas a indiferença estatística permanece. Na irradiância de 25 μmol.m-2.s-1, a 25 ± 1°C, a altura média das plântulas foi de 3,76 cm, média acima das irradiâncias da sala a 18 ± 1°C. Em relação ao número de brotações, em todas as avaliações, as irradiâncias de 35, 45 e 75 μmol.m-2.s-1 não apresentaram diferença estatística significativa. Conclui-se que diferentes irradiâncias não alteram o crescimento, mas uma menor temperatura induziu o crescimento lento no curauá, sendo uma estratégia eficaz para aumentar os intervalos de subcultivo.

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Section: Discussionunclassified

Conservação in vitro através do crescimento lento de Ananas lucidus Mill. (curauá)

Ferreira¹,

Costa²,

Silva³

et al. 2023

CLCS

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“…That requires inhibition of gibberellin biosynthesis and reduces elongation of meristematic tissues. For this purpose, scientists usually change medium composition and also used various osmotics and PGRs [50].…”

Section: Effect Of Pgrs On Micro-plantlets During Preculturementioning

confidence: 99%

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

Королева

Molkanova

Vysotskaya

2023

IJPB

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Cryopreservation is considered to be one of the most effective methods for long-term storage of plant genetic resources, particularly for ornamental species. However, there is a very little research on cryopreservation of lilacs. In this study, for the first time the cryopreservation protocol (a variation of a pregrowth-dehydration method) was successfully applied to two cultivars of Syringa vulgaris: ‘Aucubaefolia’ and ‘Polina Osipenko’. Explants of both cultivars were able to withstand the different steps of the protocol, and high survival and regrowth percentages were obtained after exposure to liquid nitrogen (67–100% and 63–88%, respectively). The current study is mainly focused on the preculture conditions of the applied method. Based on our results, we propose the use of paclobutrazol (PBZ) with the combination of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) in the preculture medium for increasing explant tolerance to subsequent dehydration and freezing. During post-LN recovery, the explants appeared morphologically normal, and after 12–16 weeks after thawing, they were propagated and cultured as normal plantlets. Therefore, the reported method is effective for long-term storage of lilac meristems and could be used to create a cryobank of achievements in lilac breeding.

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“…5D; 6B). In general, the inclusion of osmotic substances such as sucrose, mannitol (Silva and Scherwinski-Pereira, 2011;Akdemir et al, 2013), andsorbitol (Díaz-Narváez et al, 2015;Elhomosany and El-Wahab, 2020;Petrova et al, 2020;Benelli et al, 2022;Khan et al, 2022), as well as the reduction of nutrient concentration (Mohanty et al, 2013;Castilla-Valdés et al, 2020) in the culture medium, influence the subculturing interval and storage time.…”

Section: Mortalitymentioning

confidence: 99%

“…Similar findings have been documented in crops like Gypsophilla petraea and Dianthus callizanus, cultivated in media at 1/4 and 1/10, respectively, for twelve months (Catana et al, 2010). The modification of the medium concentration plays a crucial role in reducing cell division, thereby limiting proliferation and shoot growth (Benelli et al, 2022;Faisal and Alatar, 2019).…”

Section: Mortalitymentioning

confidence: 99%

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Armijos-Gonzalez,

Ramón-Contento,

Cueva-Agila

2023

Preprint

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Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. For short-term conservation, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment, cultivated in a medium with sucrose which proved useful as a cryoprotectant, followed by the encapsulation-dehydration treatment. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation.

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In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Cited by 23 publications

References 75 publications

Conservação in vitro através do crescimento lento de Ananas lucidus Mill. (curauá)

Conservação in vitro através do crescimento lento de Ananas lucidus Mill. (curauá)

Development of Cryopreservation Technique for Meristems of Syringa vulgaris L. Cultivars

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

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