Bestimmung des Fettgehalts in der Leber

Gj, Kremer; Fk, Kössling; Hj, Lange; Victor, N.

doi:10.1055/s-0028-1108920

Cited by 20 publications

(5 citation statements)

References 0 publications

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“…No data are available for comparison with the observed mono-and diglyceride levels. The cholesterol levels are within the range of those reported by other authors (6,15,23,(25)(26)(27). The increase of hepatic cholesterol levels in patients with ALD is consistent with observations by DeCarli and Lieber (28) that hepatic cholesterol levels increased slightly in rats after prolonged feeding of ethanol.…”

Section: Discussionsupporting

confidence: 91%

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Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

et al. 1983

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Alcoholic liver disease is characterized by the accumulation of fat and inflammatory changes in the liver. Because free fatty acids, the precursors of triglycerides, can damage biological membranes, accumulation of free fatty acids in the liver might be in part responsible for the functional and morphological changes seen in alcoholic liver disease. We, therefore, determined the hepatic lipid composition in biopsies from 31 patients with alcoholic liver disease, 18 patients with morbid obesity, and 5 patients without evidence of liver disease. Free fatty acids were found in all liver biopsies. Patients with morbid obesity or alcoholic liver disease had significantly higher fatty acid and triglyceride levels than did controls (p <, 0.01). Patients with alcoholic liver disease had significantly higher fatty acid levels than did patients with morbid obesity (p < 0.05), while there was no difference in the triglyceride concentrations between these two groups. The distribution of the fatty acids in the free fatty acid fraction differed significantly from that in the triglyceride fraction indicating a preferential incorporation of unsaturated fatty acids into triglycerides. This difference in the distribution pattern was lost in patients with the most severe forms of alcoholic liver disease. The data are consistent with the hypothesis that accumulation of free fatty acids in patients with alcoholic liver disease may be responsible for or contribute to the observed functional and morphological damages.

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Section: Discussionsupporting

confidence: 91%

“…The mean concentration of hepatic triglycerides is within the range of values observed by others in normal livers (6,15,23,24) and in steatosis associated with ALD or obesity (15,16,(24)(25)(26)(27). No data are available for comparison with the observed mono-and diglyceride levels.…”

Section: Discussionsupporting

confidence: 70%

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

et al. 1983

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“…The use of phospholipids as a reference substance was due to the difficulties encountered in weighing the small amounts of the biopsy material (evaporation of fluid, inclusion of washing fluid, etc.). It is generally accepted that only triglycerides increase during the development of a fatty liver (11,14, IS), while phospholipids do not change at all, or very little, which is in accordance with current views on phospholipid metabolism (1, 6). Recently published data on the triglyceride and phospholipid concentrations of human liver biopsies obtained with a Menghini needle showed a slight decrease of phospholipid values (less than 20 per cent if phospholipid mass/ (biopsy wet weightlipid mass of biopsy) was calculated) while the triglycerides increased by approx.…”

Section: Reliability Of the Methodssupporting

confidence: 52%

“…Recently published data on the triglyceride and phospholipid concentrations of human liver biopsies obtained with a Menghini needle showed a slight decrease of phospholipid values (less than 20 per cent if phospholipid mass/ (biopsy wet weightlipid mass of biopsy) was calculated) while the triglycerides increased by approx. 1500 per cent (11). The triglyceride concentration was directly proportional to the histological degree of fatty liver.…”

Section: Reliability Of the Methodsmentioning

confidence: 99%

Methods for the Determination in the Nanomole Range of Lipids in Liver Fine-Needle Aspiration Biopsies

Belfrage

Wiebe

Lundquist

1970

Scandinavian Journal of Clinical and Laboratory Investigation

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Triglycerides and phospholipids 0.5-2mg (wet weight) of human liver material obtained by the fine-needle aspiration biopsy technique can be estimated after ch1oroform:methanol extraction by the determination of lipid phosphorus and glyceride glycerol. The methods are based on the colorimetric determination of inorganic phosphate after oxidation of the phospholipids and the enzymatic fluorometric determination of glycerol after complete hydrolysis of the triglycerides, catalyzed by purified pancreatic lipase. The accumulation of lipids is then expressed as the increase in the molar ratio of triglycerides over phospholipids.

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“…Morphometric studies were conducted on 3-to 4-~m-thick sections to evaluate non-fatty and fatty parenchymous tissue and fibrous trabeculae (1 1). Biochemically estimated quantity of fat accumulation shows a high correlation with morphometric assessments (13). The analysis was performed with a Leitz Orthoplan I1 microscope equipped with a x 10 eyepiece and a x10 objective lens with a 100-point net micrometer inserted into the eyepiece.…”

Section: Methodsmentioning

confidence: 99%

Insulin-Mediated Glucose Metabolism Is Related to Liver Structure and Microsomal Function

Lahtela

Arranto

Stenbäck

et al. 1986

Scandinavian Journal of Gastroenterology

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The role of the liver in glucose metabolism was investigated in 24 consecutive patients undergoing diagnostic liver biopsy by comparing hepatic morphometry and microsomal enzyme activity in vivo (antipyrine) with fasting blood glucose (BG) and immunoreactive insulin (IRI) levels and with the metabolic clearance rate of insulin and the insulin sensitivity index. The patients had elevated BG and IRI levels and reduced insulin-mediated glucose metabolism, insulin sensitivity index, and microsomal enzyme activity as compared with controls. The insulin metabolic clearance rate did not diverge among the groups. Patients with fatty liver had a high BG associated with a reduced glucose disposal rate, whereas fasting IRI did not diverge when compared with other liver patients. Glucose disposal rate was related to the amount of unaltered liver (r2 = 0.640; p less than 0.001) and antipyrine metabolism (r = 0.631; p less than 0.01) and inversely related to the amount of fat (r2 = 0.585; p less than 0.01). The findings demonstrate that insulin-mediated glucose metabolism is related to liver structure and microsomal function. Accumulation of fat in the liver seems to be a major factor associated with reduced insulin sensitivity and glucose tolerance.

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Bestimmung des Fettgehalts in der Leber

Cited by 20 publications

References 0 publications

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

Hepatic Free Fatty Acids in Alcoholic Liver Disease and Morbid Obesity

Methods for the Determination in the Nanomole Range of Lipids in Liver Fine-Needle Aspiration Biopsies

Insulin-Mediated Glucose Metabolism Is Related to Liver Structure and Microsomal Function

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